PMID- 26031015
OWN - NLM
STAT- MEDLINE
DCOM- 20150611
LR  - 20190907
IS  - 1028-8880 (Print)
IS  - 1028-8880 (Linking)
VI  - 17
IP  - 8
DP  - 2014 Aug
TI  - Ameliorating role of rutin on oxidative stress induced by iron overload in 
      hepatic tissue of rats.
PG  - 964-77
AB  - Iron is an essential element that participates in several metabolic activities of 
      cells; however, excess iron is a major cause of iron-induced oxidative stress and 
      several human diseases. Natural flavonoids, as rutin, are well-known antioxidants 
      and could be efficient protective agents. Therefore, the present study was 
      undertaken to evaluate the protective influence of rutin supplementation to 
      improve rat antioxidant systems against IOL-induced hepatic oxidative stress. 
      Sixty male albino rats were randomly divided to three equal groups. The first 
      group, the control, the second group, iron overload group, the third group was 
      used as iron overload+rutin group. Rats received six doses of ferric hydroxide 
      polymaltose (100 mg kg(-1) b.wt.) as one dose every two days, by intraperitoneal 
      injections (IP) and administrated rutin (50 mg kg(-1) b.wt.) as one daily oral 
      dose until the sacrificed day. Blood samples for serum separation and liver 
      tissue specimens were collected three times, after three, four and five weeks 
      from the onset of the experiment. Serum iron profiles total iron, Total Iron 
      Binding Capacity (TIBC), Unsaturated Iron Binding Capacity (UIBC), transferrin 
      (Tf) and Transferrin Saturation% (TS%), ferritin, albumin, total Protein, total 
      cholesterol, triacylglycerols levels and aspartate aminotransferase (AST) and 
      alanine aminotransferase (ALT) activities were determined. Moreover, total iron 
      in the liver, L-malondialdehyde (L-MDA), glutathione (GSH), Nitric Oxide (NO) and 
      Total Nucleic Acid (TNA) levels and glutathione peroxidase (GPx), catalase (CAT) 
      and superoxide dismutase (SOD) activities were also determined. The obtained 
      results revealed that, iron overload (IOL) resulted in significant increase in 
      serum iron, TIBC, Tf, TS% and ferritin levels and AST and ALT activities and also 
      increased liver iron, L-MDA and NO levels. Meanwhile, it decreased serum UIBC, 
      total cholesterol, triacylglycerols, albumin, total protein and liver GSH, TNA 
      levels and Gpx, CAT and SOD activities when compared with the control group. 
      Rutin administration to iron-overloaded rats resulted in significant decrease in 
      serum total iron, TIBC, Tf, TS%, ferritin levels and AST and ALT activities and 
      liver total iron, L-MDA and NO levels with significant increases in serum UIBC, 
      albumin, total protein and total cholesterol levels and in liver GSH, CAT and SOD 
      activities compared with the IOL group. This study provides in vivo evidence that 
      rutin administration can improve the antioxidant defense systems against 
      IOL-induced hepatic oxidative stress in rats. This protective effect in liver of 
      iron-loaded rats may be due to both antioxidant and metal chelation activities.
FAU - Aziza, Samy Ali Hussein
AU  - Aziza SA
FAU - Azab, Mohammed El-Said
AU  - Azab Mel-S
FAU - El-Shall, Soheir Kamal
AU  - El-Shall SK
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - Pak J Biol Sci
JT  - Pakistan journal of biological sciences : PJBS
JID - 101247723
RN  - 5G06TVY3R7 (Rutin)
SB  - IM
MH  - Animals
MH  - Iron Overload/*metabolism
MH  - Liver/*metabolism
MH  - Male
MH  - Oxidative Stress/*drug effects
MH  - Rats
MH  - Rutin/*pharmacology
EDAT- 2015/06/03 06:00
MHDA- 2015/06/13 06:00
CRDT- 2015/06/03 06:00
PHST- 2015/06/03 06:00 [entrez]
PHST- 2015/06/03 06:00 [pubmed]
PHST- 2015/06/13 06:00 [medline]
AID - 10.3923/pjbs.2014.964.977 [doi]
PST - ppublish
SO  - Pak J Biol Sci. 2014 Aug;17(8):964-77. doi: 10.3923/pjbs.2014.964.977.