PMID- 26031202
OWN - NLM
STAT- MEDLINE
DCOM- 20150818
LR  - 20181113
IS  - 1365-2613 (Electronic)
IS  - 0959-9673 (Print)
IS  - 0959-9673 (Linking)
VI  - 96
IP  - 2
DP  - 2015 Apr
TI  - A novel technique for simultaneous whole-body and multi-organ decellularization: 
      umbilical artery catheterization as a perfusion-based method in a sheep foetus 
      model.
PG  - 116-32
LID - 10.1111/iep.12124 [doi]
AB  - The aim of this study was to develop a method to generate multi-organ acellular 
      matrices. Using a foetal sheep model have developed a method of systemic 
      pulsatile perfusion via the umbilical artery which allows for simultaneous 
      multi-organ decellularization. Twenty sheep foetuses were systemically perfused 
      with Triton X-100 and sodium dodecyl sulphate. Following completion of the 
      whole-body decellularization, multiple biopsy samples were taken from different 
      parts of 21 organs to ascertain complete cell component removal in the preserved 
      extracellular matrices. Both the natural and decellularized organs were subjected 
      to several examinations. The samples were obtained from the skin, eye, ear, nose, 
      throat, cardiovascular, respiratory, gastrointestinal, urinary, musculoskeletal, 
      central nervous and peripheral nervous systems. The histological results depicted 
      well-preserved extracellular matrix (ECM) integrity and intact vascular 
      structures, without any evidence of residual cellular materials, in all 
      decellularized bioscaffolds. Scanning electron microscope (SEM) and biochemical 
      properties remained intact, similar to their age-matched native counterparts. 
      Preservation of the collagen structure was evaluated by a hydroxyproline assay. 
      Dense organs such as bone and muscle were also completely decellularized, with a 
      preserved ECM structure. Thus, as shown in this study, several organs and 
      different tissues were decellularized using a perfusion-based method, which has 
      not been previously accomplished. Given the technical challenges that exist for 
      the efficient generation of biological scaffolds, the current results may pave 
      the way for obtaining a variety of decellularized scaffolds from a single donor. 
      In this study, there have been unique responses to the single acellularization 
      protocol in foetuses, which may reflect the homogeneity of tissues and organs in 
      the developing foetal body.
CI  - (c) 2015 The Authors. International Journal of Experimental Pathology (c) 2015 
      International Journal of Experimental Pathology.
FAU - Kajbafzadeh, Abdol-Mohammad
AU  - Kajbafzadeh AM
AD  - Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells 
      Therapy, Children's Hospital Medical Center, Tehran University of Medical 
      Sciences, Tehran, Iran (IRI).
FAU - Khorramirouz, Reza
AU  - Khorramirouz R
AD  - Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells 
      Therapy, Children's Hospital Medical Center, Tehran University of Medical 
      Sciences, Tehran, Iran (IRI).
FAU - Akbarzadeh, Aram
AU  - Akbarzadeh A
AD  - Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells 
      Therapy, Children's Hospital Medical Center, Tehran University of Medical 
      Sciences, Tehran, Iran (IRI).
FAU - Sabetkish, Shabnam
AU  - Sabetkish S
AD  - Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells 
      Therapy, Children's Hospital Medical Center, Tehran University of Medical 
      Sciences, Tehran, Iran (IRI).
FAU - Sabetkish, Nastaran
AU  - Sabetkish N
AD  - Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells 
      Therapy, Children's Hospital Medical Center, Tehran University of Medical 
      Sciences, Tehran, Iran (IRI).
FAU - Saadat, Paria
AU  - Saadat P
AD  - Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells 
      Therapy, Children's Hospital Medical Center, Tehran University of Medical 
      Sciences, Tehran, Iran (IRI).
FAU - Tehrani, Mona
AU  - Tehrani M
AD  - Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells 
      Therapy, Children's Hospital Medical Center, Tehran University of Medical 
      Sciences, Tehran, Iran (IRI).
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Exp Pathol
JT  - International journal of experimental pathology
JID - 9014042
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 9002-93-1 (Octoxynol)
SB  - IM
MH  - Angiography
MH  - Animal Structures/*cytology/drug effects
MH  - Animals
MH  - Blood Vessels/cytology/ultrastructure
MH  - Catheterization/*methods
MH  - Extracellular Matrix/ultrastructure
MH  - Female
MH  - Fetus/*cytology/drug effects
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Microscopy, Electron, Scanning
MH  - Models, Animal
MH  - Octoxynol/*administration & dosage/pharmacology
MH  - Perfusion/*methods
MH  - Pregnancy
MH  - Sheep
MH  - Sodium Dodecyl Sulfate/*administration & dosage/pharmacology
MH  - Tissue Engineering/*methods
MH  - *Tissue Scaffolds
MH  - Tomography, X-Ray Computed
MH  - Umbilical Arteries
PMC - PMC4459805
OTO - NOTNLM
OT  - extracellular matrix
OT  - foetus
OT  - scaffolds
OT  - sheep
OT  - whole-body decellularization
EDAT- 2015/06/03 06:00
MHDA- 2015/08/19 06:00
PMCR- 2016/04/01
CRDT- 2015/06/03 06:00
PHST- 2014/09/12 00:00 [received]
PHST- 2015/02/09 00:00 [accepted]
PHST- 2015/06/03 06:00 [entrez]
PHST- 2015/06/03 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - 10.1111/iep.12124 [doi]
PST - ppublish
SO  - Int J Exp Pathol. 2015 Apr;96(2):116-32. doi: 10.1111/iep.12124.