PMID- 26032513
OWN - NLM
STAT- MEDLINE
DCOM- 20150824
LR  - 20191210
IS  - 1522-1555 (Electronic)
IS  - 0193-1849 (Linking)
VI  - 308
IP  - 11
DP  - 2015 Jun 1
TI  - Late-onset caloric restriction alters skeletal muscle metabolism by modulating 
      pyruvate metabolism.
PG  - E942-9
LID - 10.1152/ajpendo.00508.2014 [doi]
AB  - Caloric restriction (CR) attenuates age-related muscle loss. However, the 
      underlying mechanism responsible for this attenuation is not fully understood. 
      This study evaluated the role of energy metabolism in the CR-induced attenuation 
      of muscle loss. The aims of this study were twofold: 1) to evaluate the effect of 
      CR on energy metabolism and determine its relationship with muscle mass, and 2) 
      to determine whether the effects of CR are age dependent. Young and middle-aged 
      rats were randomized into either 40% CR or ad libitum (AL) diet groups for 14 wk. 
      Major energy-producing pathways in muscles, i.e., glycolysis and mitochondrial 
      oxidative phosphorylation (OXPHOS), were examined. We found that the effects of 
      CR were age dependent. CR improved muscle metabolism and normalized muscle mass 
      in middle-aged animals but not young animals. CR decreased glycolysis and 
      increased the cellular dependency for OXPHOS vs. glycolysis in muscles of 
      middle-aged rats, which was associated with the improvement of normalized muscle 
      mass. The metabolic reprogramming induced by CR was related to modulation of 
      pyruvate metabolism and increased mitochondrial biogenesis. Compared with animals 
      fed AL, middle-aged animals with CR had lower lactate dehydrogenase A content and 
      greater mitochondrial pyruvate carrier content. Markers of mitochondrial 
      biogenesis, including AMPK activation levels and SIRT1 and COX-IV content, also 
      showed increased levels. In conclusion, 14 wk of CR improved muscle metabolism 
      and preserved muscle mass in middle-aged animals but not in young developing 
      animals. CR-attenuated age-related muscle loss is associated with reprogramming 
      of the metabolic pathway from glycolysis to OXPHOS.
CI  - Copyright (c) 2015 the American Physiological Society.
FAU - Chen, Chiao-Nan Joyce
AU  - Chen CN
AD  - Department of Physical Therapy, Medical School, Chang Gung University, Tao-Yuan, 
      Taiwan; Healthy Aging Research Center, Chang Gung University, Tao-Yuan, Taiwan; 
      cnchen@mail.cgu.edu.tw.
FAU - Lin, Shang-Ying
AU  - Lin SY
AD  - Department of Physical Therapy, Medical School, Chang Gung University, Tao-Yuan, 
      Taiwan;
FAU - Liao, Yi-Hung
AU  - Liao YH
AD  - Department of Exercise and Health Science, College of Human Development and 
      Health, National Taipei University of Nursing and Health Sciences, Taipei, 
      Taiwan; and.
FAU - Li, Zhen-Jie
AU  - Li ZJ
AD  - Department of Physical Therapy, Medical School, Chang Gung University, Tao-Yuan, 
      Taiwan;
FAU - Wong, Alice May-Kuen
AU  - Wong AM
AD  - Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, 
      Taipei, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150414
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Isoenzymes)
RN  - 8558G7RUTR (Pyruvic Acid)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.27.- (Lactate Dehydrogenase 5)
SB  - IM
MH  - Age Factors
MH  - Aging/*metabolism
MH  - Animals
MH  - *Caloric Restriction
MH  - Energy Metabolism
MH  - Isoenzymes/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Lactate Dehydrogenase 5
MH  - Mitochondria, Muscle/metabolism
MH  - Muscle, Skeletal/anatomy & histology/*metabolism
MH  - Organ Size
MH  - Pyruvic Acid/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - dietary restriction
OT  - glycolysis
OT  - lactate dehydrogenase
OT  - mitochondrial pyruvate carrier
OT  - muscle
EDAT- 2015/06/03 06:00
MHDA- 2015/08/25 06:00
CRDT- 2015/06/03 06:00
PHST- 2014/11/07 00:00 [received]
PHST- 2015/04/07 00:00 [accepted]
PHST- 2015/06/03 06:00 [entrez]
PHST- 2015/06/03 06:00 [pubmed]
PHST- 2015/08/25 06:00 [medline]
AID - ajpendo.00508.2014 [pii]
AID - 10.1152/ajpendo.00508.2014 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2015 Jun 1;308(11):E942-9. doi: 
      10.1152/ajpendo.00508.2014. Epub 2015 Apr 14.