PMID- 26034532
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150602
LR  - 20201001
IS  - 2008-3645 (Print)
IS  - 2008-4161 (Electronic)
IS  - 2008-3645 (Linking)
VI  - 8
IP  - 4
DP  - 2015 Apr
TI  - Isolation and Biochemical Fingerprinting of Vancomycin-Resistant Enterococcus 
      faecium From Meat, Chicken and Cheese.
PG  - e15815
LID - 10.5812/jjm.8(4)2015.15815 [doi]
LID - e15815
AB  - BACKGROUND: Vancomycin-resistant enterococci (VRE) are important nosocomial 
      pathogens and food chain has been considered as an assumed source for 
      dissemination of VRE to human. OBJECTIVES: The presence of VRE isolates from food 
      samples and typing of these isolates with Phene plate, a biochemical 
      fingerprinting method, were investigated. MATERIALS AND METHODS: Thirty samples 
      of meat, chicken and cheese were analyzed for VRE during 2010. Antibiotic 
      susceptibility tests and minimum inhibitory concentration (MIC) were also 
      examined. VRE isolates were typed with the Phene plate system (PhPlate), a 
      biochemical fingerprinting method. RESULTS: A total of 70 VRE isolates were 
      obtained and identified as Enterococcus faecium by species-specific PCR. All the 
      isolates carried vanA, while none of them harbored vanB. The VRE isolates 
      included 35, 27, and 8 isolates from meat, chicken and cheese, respectively. 
      Typing with the PhPlate revealed a diversity index of 0.78 for E. faecium, 
      containing 10 common and four single types. The results of antibiotic 
      susceptibility and MIC tests showed an increased resistance to ciprofloxacin, 
      erythromycin, ampicillin and gentamicin, to which, 100%, 100%, 100%, and 95% of 
      VRE isolates were resistant, respectively. Only 5% of the isolates were resistant 
      to chloramphenicol and the MIC of the isolates for vancomycin and teicoplanin was 
      >/= 256 microg/mL and for gentamicin-resistant isolates it was 1024 microg/mL. Conventional 
      and molecular identification tests exhibited that all the isolates were E. 
      faecium carrying vanA. None of the isolates harbored vanB. CONCLUSIONS: The 
      results showed that enterococci are common contaminants in food. Indeed, this 
      study indicates a high prevalence of multidrug-resistant enterococci in food of 
      animal origin in Iran. Isolating some persisting enterococcal isolates revealed 
      that continuous surveillance of antimicrobial resistance in enterococci from food 
      is essential.
FAU - Talebi, Malihe
AU  - Talebi M
AD  - Department of Microbiology, School of Medicine, Iran University of Medical 
      Sciences, Tehran, IR Iran.
FAU - Sadeghi, Javad
AU  - Sadeghi J
AD  - Department of Microbiology, School of Medicine, Tehran University of Medical 
      Sciences , Tehran, IR Iran.
FAU - Rahimi, Fateh
AU  - Rahimi F
AD  - Department of Microbiology, Faculty of Science, University of Isfahan, Isfahan, 
      IR Iran.
FAU - Pourshafie, Mohammad Reza
AU  - Pourshafie MR
AD  - Department of Microbiology, Pasteur Institute of Iran, Tehran, IR Iran.
LA  - eng
PT  - Journal Article
DEP - 20150418
PL  - Netherlands
TA  - Jundishapur J Microbiol
JT  - Jundishapur journal of microbiology
JID - 101515122
PMC - PMC4449848
OTO - NOTNLM
OT  - Fingerprinting
OT  - Food
OT  - Minimum Inhibitory Concentration
OT  - Multidrug Resistance
OT  - Vancomycin-Resistant Enterococci
EDAT- 2015/06/03 06:00
MHDA- 2015/06/03 06:01
PMCR- 2015/04/01
CRDT- 2015/06/03 06:00
PHST- 2013/10/30 00:00 [received]
PHST- 2014/01/18 00:00 [revised]
PHST- 2014/02/12 00:00 [accepted]
PHST- 2015/06/03 06:00 [entrez]
PHST- 2015/06/03 06:00 [pubmed]
PHST- 2015/06/03 06:01 [medline]
PHST- 2015/04/01 00:00 [pmc-release]
AID - 10.5812/jjm.8(4)2015.15815 [doi]
PST - epublish
SO  - Jundishapur J Microbiol. 2015 Apr 18;8(4):e15815. doi: 
      10.5812/jjm.8(4)2015.15815. eCollection 2015 Apr.