PMID- 26035250
OWN - NLM
STAT- MEDLINE
DCOM- 20151207
LR  - 20220727
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 63
IP  - 23
DP  - 2015 Jun 17
TI  - Human Ether-a-go-go Related Gene (hERG) Channel Blocking Aporphine Alkaloids from 
      Lotus Leaves and Their Quantitative Analysis in Dietary Weight Loss Supplements.
PG  - 5634-9
LID - 10.1021/acs.jafc.5b01901 [doi]
AB  - Blockage of the human ether-a-go-go related gene (hERG) channel can result in 
      life-threatening ventricular tachyarrhythmia. In an in vitro screening of herbal 
      materials for hERG blockers using an automated two-microelectrode voltage clamp 
      assay on Xenopus oocytes, an alkaloid fraction of Nelumbo nucifera Gaertn. 
      (lotus) leaves induced  approximately 50% of hERG current inhibition at 100 mug/mL. 
      Chromatographic separation resulted in the isolation and identification of 
      (-)-asimilobine, 1, nuciferine, 2, O-nornuciferine, 3, N-nornuciferine, 4, and 
      liensinine, 5. In agreement with in silico predicted ligand-target interactions, 
      2, 3, and 4 revealed distinct in vitro hERG blockages measured in HEK293 cells 
      with IC50 values of 2.89, 7.91, and 9.75 muM, respectively. Because lotus leaf 
      dietary weight loss supplements are becoming increasingly popular, the identified 
      hERG-blocking alkaloids were quantitated in five commercially available products. 
      Results showed pronounced differences in the content of hERG-blocking alkaloids 
      ranging up to 992 mug (2) in the daily recommended dose.
FAU - Grienke, Ulrike
AU  - Grienke U
AD  - daggerInstitute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, 
      University of Innsbruck, Innrain 80-82, 6020 Innsbruck, Austria.
FAU - Mair, Christina E
AU  - Mair CE
AD  - daggerInstitute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, 
      University of Innsbruck, Innrain 80-82, 6020 Innsbruck, Austria.
FAU - Saxena, Priyanka
AU  - Saxena P
FAU - Baburin, Igor
AU  - Baburin I
FAU - Scheel, Olaf
AU  - Scheel O
AD  - #Cytocentrics Bioscience GmbH, Tannenweg 22k, 18059 Rostock, Germany.
FAU - Ganzera, Markus
AU  - Ganzera M
AD  - daggerInstitute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, 
      University of Innsbruck, Innrain 80-82, 6020 Innsbruck, Austria.
FAU - Schuster, Daniela
AU  - Schuster D
AD  -  perpendicularInstitute of Pharmacy/Pharmaceutical Chemistry, Computer-Aided Molecular Design 
      Group, Center for Molecular Biosciences Innsbruck, University of Innsbruck, 
      Innrain 80-82, 6020 Innsbruck, Austria.
FAU - Hering, Steffen
AU  - Hering S
FAU - Rollinger, Judith M
AU  - Rollinger JM
AD  - daggerInstitute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, 
      University of Innsbruck, Innrain 80-82, 6020 Innsbruck, Austria.
LA  - eng
GR  - W 1232/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150609
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Alkaloids)
RN  - 0 (Anti-Obesity Agents)
RN  - 0 (Aporphines)
RN  - 0 (Ether-A-Go-Go Potassium Channels)
RN  - 0 (Plant Extracts)
RN  - 0 (Potassium Channel Blockers)
RN  - 13NS2KTD6H (aporphine)
SB  - IM
MH  - Alkaloids/*chemistry/pharmacology
MH  - Animals
MH  - Anti-Obesity Agents/*chemistry/pharmacology
MH  - Aporphines/*chemistry/pharmacology
MH  - Cell Line
MH  - Dietary Supplements/*analysis
MH  - Ether-A-Go-Go Potassium Channels/*antagonists & inhibitors/metabolism
MH  - Humans
MH  - Nelumbo/*chemistry
MH  - Plant Extracts/*chemistry/pharmacology
MH  - Plant Leaves/*chemistry
MH  - Potassium Channel Blockers/*chemistry/pharmacology
MH  - Xenopus
OTO - NOTNLM
OT  - Nelumbo nucifera
OT  - cardiotoxic risk
OT  - dietary supplement
OT  - hERG
OT  - lotus
OT  - nuciferine
OT  - patch clamp assay
OT  - weight loss
EDAT- 2015/06/03 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/06/03 06:00
PHST- 2015/06/03 06:00 [entrez]
PHST- 2015/06/03 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1021/acs.jafc.5b01901 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2015 Jun 17;63(23):5634-9. doi: 10.1021/acs.jafc.5b01901. Epub 
      2015 Jun 9.