PMID- 26035417
OWN - NLM
STAT- MEDLINE
DCOM- 20160330
LR  - 20201209
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 36
IP  - 2
DP  - 2015 Aug
TI  - Elemol from Chamaecyparis obtusa ameliorates 2,4-dinitrochlorobenzene-induced 
      atopic dermatitis.
PG  - 463-72
LID - 10.3892/ijmm.2015.2228 [doi]
AB  - Chamaecyparis obtusa has been traditionally used as an antibiotic agent and in 
      cosmetics for the prevention of microorganism infection and skin troubles. Atopic 
      dermatitis (AD) is a chronic inflammatory skin disease that encompasses 
      immunologic responses, susceptibility factors and compromised skin-barrier 
      function. Use of plant medicines in therapeutic treatment of AD has recently been 
      suggested as an alternative therapeutic option. The present study examined the 
      effect of elemol, an active component of Chamaecyparis obtusa, on AD using in 
      vivo and in vitro models. RBL-2H3 cells were stimulated with concanavalin A and 
      dinitrophenyl human serum albumin, and atopic dermatitis was induced in BALB/c 
      mice by topical application of 2,4-dinitrochlorobenzene (DNCB) prior to elemol 
      treatment. The mRNA expression was evaluated by reverse transcription 
      quantitative polymerase chain reaction, and the levels of beta-hexosaminidase and 
      serum immunoglobulin E (IgE) were examined by ELISA. Histological changes were 
      also performed by microscopy. Elemol attenuated the onset of AD-like skin 
      lesions, reduced serum IgE levels and decreased mast cell infiltration into the 
      dermis and hypodermis. In addition, elemol downregulated the transcriptional 
      expression of several pro-inflammatory cytokines, including TNF-alpha, IL-1beta, IL-6 
      and IkappaBalpha, in the skin of the DNCB-induced animal models of AD. In the RBL-2H3 
      mast cell line, elemol significantly inhibited the mRNA expression of IL-4 and 
      IL-13, and further attenuated the release of beta-hexosaminidase from mast cells. 
      Histological examination revealed that elemol significantly ameliorated the 
      DNCB-induced dermal destruction in mice. The results of the present study 
      suggested that elemol may have therapeutic potential in the treatment of AD due 
      to its immunosuppressive effects.
FAU - Yang, Hyun
AU  - Yang H
AD  - Laboratory of Veterinary Biochemistry and Molecular Biology, College of 
      Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, 
      Republic of Korea.
FAU - Jung, Eui-Man
AU  - Jung EM
AD  - Laboratory of Veterinary Biochemistry and Molecular Biology, College of 
      Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, 
      Republic of Korea.
FAU - Ahn, Changhwan
AU  - Ahn C
AD  - Laboratory of Veterinary Biochemistry and Molecular Biology, College of 
      Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, 
      Republic of Korea.
FAU - Lee, Geun-Shik
AU  - Lee GS
AD  - Laboratory of Veterinary Physiology, College of Veterinary Medicine and Institute 
      of Veterinary Science, Kangwon National University, Chuncheon, Gangwon 200-701, 
      Republic of Korea.
FAU - Lee, Su-Yeon
AU  - Lee SY
AD  - Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul 
      National University, Seoul 151-921, Republic of Korea.
FAU - Kim, Seon-Hong
AU  - Kim SH
AD  - Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul 
      National University, Seoul 151-921, Republic of Korea.
FAU - Choi, In-Gyu
AU  - Choi IG
AD  - Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul 
      National University, Seoul 151-921, Republic of Korea.
FAU - Park, Mi-Jin
AU  - Park MJ
AD  - Division of Wood Chemistry and Microbiology, Department of Forest Products, Korea 
      Forest Research Institute, Seoul 130-712, Republic of Korea.
FAU - Lee, Sung-Suk
AU  - Lee SS
AD  - Division of Wood Chemistry and Microbiology, Department of Forest Products, Korea 
      Forest Research Institute, Seoul 130-712, Republic of Korea.
FAU - Choi, Don-Ha
AU  - Choi DH
AD  - Division of Wood Chemistry and Microbiology, Department of Forest Products, Korea 
      Forest Research Institute, Seoul 130-712, Republic of Korea.
FAU - Jeung, Eui-Bae
AU  - Jeung EB
AD  - Laboratory of Veterinary Biochemistry and Molecular Biology, College of 
      Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, 
      Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150529
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Cytokines)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Oils, Volatile)
RN  - 0 (Sesquiterpenes)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - L92AJ7G06I (elemol)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chamaecyparis/*chemistry
MH  - Cytokines/analysis
MH  - Dermatitis, Atopic/blood/*chemically induced/*drug therapy/pathology
MH  - *Dinitrochlorobenzene
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Oils, Volatile/chemistry/therapeutic use
MH  - Sesquiterpenes/chemistry/*therapeutic use
MH  - Skin/*drug effects/*pathology
EDAT- 2015/06/03 06:00
MHDA- 2016/03/31 06:00
CRDT- 2015/06/03 06:00
PHST- 2014/11/19 00:00 [received]
PHST- 2015/05/21 00:00 [accepted]
PHST- 2015/06/03 06:00 [entrez]
PHST- 2015/06/03 06:00 [pubmed]
PHST- 2016/03/31 06:00 [medline]
AID - 10.3892/ijmm.2015.2228 [doi]
PST - ppublish
SO  - Int J Mol Med. 2015 Aug;36(2):463-72. doi: 10.3892/ijmm.2015.2228. Epub 2015 May 
      29.