PMID- 26037339
OWN - NLM
STAT- MEDLINE
DCOM- 20150814
LR  - 20191210
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 32
IP  - 3
DP  - 2015 Jun
TI  - [The value of blastocyst culture on preimplantation genetic diagnosis].
PG  - 312-7
LID - 10.3760/cma.j.issn.1003-9406.2015.03.002 [doi]
AB  - OBJECTIVE: To estimate the value of blastocyst culture for preimplantation 
      genetic diagnosis (PGD). METHODS: Day 3 embryos were biopsied and analyzed with 
      fluorescence in situ hybridization (FISH) technique. Embryos with normal FISH 
      results were cultured into blastocysts, and the ones with better morphology 
      scores were transferred. Fourteen embryos with abnormal FISH results were 
      cultured into blastocysts. Part of the cells taken from the blastocysts were 
      amplified by whole genomic amplification (WGA) and assessed by array-based 
      comparative genomic hybridization (array-CGH) analysis. RESULTS: Six blastocysts 
      with normal FISH results were transferred in 5 cycles. Four healthy babies of 3 
      cycles were delivered. Another one was a singleton pregnancy but with embryo 
      growth arrest, whose villus karyotype was normal. Fourteen embryos with abnormal 
      FISH results were cultured into blastocysts and analyzed by array-CGH. Six 
      blastocysts were normal by array-CGH. CONCLUSION: FISH combined with blastocyst 
      culture may further ensure the accuracy of PGD result. Detection at the 
      blastocyst stage can avoid false positive results and mosaic interferences on Day 
      3 stage and are therefore more authentic.
FAU - Ou, Jian
AU  - Ou J
AD  - Center of Reproduction and Genetics, Suzhou Municipal Hospital, Suzhou, Jiangsu 
      215002, P.R.China. hongliszivf@163.com; richardchoy@cuhk.edu.hk.
FAU - Wang, Wei
AU  - Wang W
FAU - Ma, Yanlin
AU  - Ma Y
FAU - Zhou, Zhi
AU  - Zhou Z
FAU - Ding, Jie
AU  - Ding J
FAU - Wang, Fuxin
AU  - Wang F
FAU - Duan, Chengying
AU  - Duan C
FAU - Li, Linjiang
AU  - Li L
FAU - Zheng, Aiyan
AU  - Zheng A
FAU - Chong, Wilson
AU  - Chong W
FAU - Choy, Richard
AU  - Choy R
FAU - Li, Hong
AU  - Li H
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Blastocyst/*cytology
MH  - Comparative Genomic Hybridization/*methods
MH  - Embryo Transfer
MH  - Female
MH  - Genetic Diseases, Inborn/*diagnosis/embryology/genetics/prevention & control
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Pregnancy
MH  - Preimplantation Diagnosis/*methods
EDAT- 2015/06/04 06:00
MHDA- 2015/08/15 06:00
CRDT- 2015/06/04 06:00
PHST- 2015/06/04 06:00 [entrez]
PHST- 2015/06/04 06:00 [pubmed]
PHST- 2015/08/15 06:00 [medline]
AID - 940632067 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2015.03.002 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2015 Jun;32(3):312-7. doi: 
      10.3760/cma.j.issn.1003-9406.2015.03.002.