PMID- 26037506
OWN - NLM
STAT- MEDLINE
DCOM- 20170317
LR  - 20170317
IS  - 1552-5732 (Electronic)
IS  - 0890-3344 (Linking)
VI  - 32
IP  - 3
DP  - 2016 Aug
TI  - Calibration Adjustment of the Mid-infrared Analyzer for an Accurate Determination 
      of the Macronutrient Composition of Human Milk.
PG  - NP19-27
LID - 10.1177/0890334415588513 [doi]
AB  - BACKGROUND: Human milk composition analysis seems essential to adapt human milk 
      fortification for preterm neonates. The Miris human milk analyzer (HMA), based on 
      mid-infrared methodology, is convenient for a unique determination of 
      macronutrients. However, HMA measurements are not totally comparable with 
      reference methods (RMs). OBJECTIVE: The primary aim of this study was to compare 
      HMA results with results from biochemical RMs for a large range of protein, fat, 
      and carbohydrate contents and to establish a calibration adjustment. METHODS: 
      Human milk was fractionated in protein, fat, and skim milk by covering large 
      ranges of protein (0-3 g/100 mL), fat (0-8 g/100 mL), and carbohydrate (5-8 g/100 
      mL). For each macronutrient, a calibration curve was plotted by linear regression 
      using measurements obtained using HMA and RMs. RESULTS: For fat, 53 measurements 
      were performed, and the linear regression equation was HMA = 0.79RM + 0.28 (R(2) 
      = 0.92). For true protein (29 measurements), the linear regression equation was 
      HMA = 0.9RM + 0.23 (R(2) = 0.98). For carbohydrate (15 measurements), the linear 
      regression equation was HMA = 0.59RM + 1.86 (R(2) = 0.95). A homogenization step 
      with a disruptor coupled to a sonication step was necessary to obtain better 
      accuracy of the measurements. Good repeatability (coefficient of variation < 7%) 
      and reproducibility (coefficient of variation < 17%) were obtained after 
      calibration adjustment. CONCLUSION: New calibration curves were developed for the 
      Miris HMA, allowing accurate measurements in large ranges of macronutrient 
      content. This is necessary for reliable use of this device in individualizing 
      nutrition for preterm newborns.
CI  - (c) The Author(s) 2015.
FAU - Billard, Helene
AU  - Billard H
AD  - INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, Nantes, France 
      Centre de Recherche en Nutrition Humaine - Ouest, Nantes, France L'Universite 
      Nantes Angers Le Mans, Nantes, France.
FAU - Simon, Laure
AU  - Simon L
AD  - INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, Nantes, France 
      Centre de Recherche en Nutrition Humaine - Ouest, Nantes, France L'Universite 
      Nantes Angers Le Mans, Nantes, France Centre Hospitalo-Universitaire Hotel-Dieu, 
      Nantes, France.
FAU - Desnots, Emmanuelle
AU  - Desnots E
AD  - INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, Nantes, France 
      Centre de Recherche en Nutrition Humaine - Ouest, Nantes, France L'Universite 
      Nantes Angers Le Mans, Nantes, France.
FAU - Sochard, Agnes
AU  - Sochard A
AD  - INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, Nantes, France 
      Centre de Recherche en Nutrition Humaine - Ouest, Nantes, France L'Universite 
      Nantes Angers Le Mans, Nantes, France.
FAU - Boscher, Cecile
AU  - Boscher C
AD  - Centre Hospitalo-Universitaire Hotel-Dieu, Nantes, France.
FAU - Riaublanc, Alain
AU  - Riaublanc A
AD  - INRA, UR 1268 BIA (Biopolymeres, Interactions, Assemblages), Nantes, France.
FAU - Alexandre-Gouabau, Marie-Cecile
AU  - Alexandre-Gouabau MC
AD  - INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, Nantes, France 
      Centre de Recherche en Nutrition Humaine - Ouest, Nantes, France L'Universite 
      Nantes Angers Le Mans, Nantes, France.
FAU - Boquien, Clair-Yves
AU  - Boquien CY
AD  - INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, Nantes, France 
      Centre de Recherche en Nutrition Humaine - Ouest, Nantes, France L'Universite 
      Nantes Angers Le Mans, Nantes, France clair-yves.boquien@univ-nantes.fr.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20150602
PL  - United States
TA  - J Hum Lact
JT  - Journal of human lactation : official journal of International Lactation 
      Consultant Association
JID - 8709498
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Fats)
RN  - 0 (Milk Proteins)
MH  - Calibration
MH  - Dietary Carbohydrates/*analysis
MH  - Dietary Fats/*analysis
MH  - Humans
MH  - Linear Models
MH  - Milk Proteins/*analysis
MH  - Milk, Human/*chemistry
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Spectrophotometry, Infrared/instrumentation/*standards
OTO - NOTNLM
OT  - breastfeeding
OT  - human milk
OT  - milk composition
OT  - premature infants
EDAT- 2015/06/04 06:00
MHDA- 2017/03/18 06:00
CRDT- 2015/06/04 06:00
PHST- 2014/09/23 00:00 [received]
PHST- 2015/05/03 00:00 [accepted]
PHST- 2015/06/04 06:00 [entrez]
PHST- 2015/06/04 06:00 [pubmed]
PHST- 2017/03/18 06:00 [medline]
AID - 0890334415588513 [pii]
AID - 10.1177/0890334415588513 [doi]
PST - ppublish
SO  - J Hum Lact. 2016 Aug;32(3):NP19-27. doi: 10.1177/0890334415588513. Epub 2015 Jun 
      2.