PMID- 26037627
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1476-4954 (Electronic)
IS  - 1476-4954 (Linking)
VI  - 29
IP  - 7
DP  - 2016
TI  - T regulatory markers expression in unexplained recurrent spontaneous abortion.
PG  - 1175-80
LID - 10.3109/14767058.2015.1039507 [doi]
AB  - OBJECTIVE: To evaluate expression of glucocorticoid-induced tumor necrosis factor 
      receptor (GITR), cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and IL-10 
      in peripheral blood mononuclear cells (PBMCs) of 20 women with unexplained 
      recurrent spontaneous abortion (URSA) compared to 20 normal non-pregnant women 
      (NNP) during luteal phase in the window of implantation. METHODS: Quantitative 
      real-time PCR (qRT-PCR) was performed using the Taqman method for expression of 
      GITR and SYBR Green method for expression of CTLA-4 and IL-10. RESULTS: 
      Expression of CTLA-4 in the NNPs (median; interquartile range; 3; 1.8-10) was 
      significantly higher than the URSAs (0.72; 0.26-3.81, p = 0.015). Expression of 
      GITR in the NNPs (53; 10-139) was significantly higher than the URSAs (6; 3-27, p 
      = 0.005). However, IL-10 expression in the URSAs was significantly higher than 
      the NNPs, did not meet a significant value. A significant correlation was found 
      between CTLA-4 and GITR expression in the study population (p = 0.0001). 
      CONCLUSIONS: Expression of CTLA-4 and GITR were significantly down-regulated in 
      the URSAs compared to NNPs at the window of implantation, which shows the 
      essential role of Treg cells in creating an immunological privileged site for 
      fetus as an allograft at the maternal-fetal interface by high expression levels 
      of CTLA-4 and GITR during a normal pregnancy.
FAU - Saifi, Bita
AU  - Saifi B
AD  - a Department of Anatomical Sciences, Faculty of Medicine , Iran University of 
      Medical Sciences , Tehran , Iran .
AD  - b Department of Anatomical Sciences, Mashhad Medical Science Branch , Islamic 
      Azad University , Mashhad , Iran .
FAU - Aflatoonian, Reza
AU  - Aflatoonian R
AD  - c Department of Endocrinology and Female Infertility at Reproductive Biomedicine 
      Research Center , Royan Institute for Reproductive Biomedicine, ACECR , Tehran , 
      Iran .
FAU - Tajik, Nader
AU  - Tajik N
AD  - d Department of Immunology, Faculty of Medicine, Division of Immunogenetics , 
      Iran University of Medical Sciences , Tehran , Iran .
FAU - Erfanian Ahmadpour, Mahmood
AU  - Erfanian Ahmadpour M
AD  - b Department of Anatomical Sciences, Mashhad Medical Science Branch , Islamic 
      Azad University , Mashhad , Iran .
FAU - Vakili, Rosita
AU  - Vakili R
AD  - e Center of Pathological and Medical Diagnostic Services, Iranian Academic Center 
      for Education, Culture & Research (ACECR), Mashhad Branch , Mashhad , Iran .
FAU - Amjadi, Fatemehsadat
AU  - Amjadi F
AD  - a Department of Anatomical Sciences, Faculty of Medicine , Iran University of 
      Medical Sciences , Tehran , Iran .
FAU - Valizade, Narges
AU  - Valizade N
AD  - f Immunology Research Center , Faculty of Medicine , Mashhad University of 
      Medical Sciences , Mashhad , Iran , and.
FAU - Ahmadi, Sanaz
AU  - Ahmadi S
AD  - e Center of Pathological and Medical Diagnostic Services, Iranian Academic Center 
      for Education, Culture & Research (ACECR), Mashhad Branch , Mashhad , Iran .
FAU - Rezaee, Seyed Abdolrahim
AU  - Rezaee SA
AD  - f Immunology Research Center , Faculty of Medicine , Mashhad University of 
      Medical Sciences , Mashhad , Iran , and.
FAU - Mehdizadeh, Mehdi
AU  - Mehdizadeh M
AD  - g Cellular and Molecular Research Center, Faculty of Advanced Technologies in 
      Medicine, Iran University of Medical Sciences , Tehran , Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150602
PL  - England
TA  - J Matern Fetal Neonatal Med
JT  - The journal of maternal-fetal & neonatal medicine : the official journal of the 
      European Association of Perinatal Medicine, the Federation of Asia and Oceania 
      Perinatal Societies, the International Society of Perinatal Obstetricians
JID - 101136916
RN  - 0 (Biomarkers)
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (CTLA4 protein, human)
RN  - 0 (Glucocorticoid-Induced TNFR-Related Protein)
RN  - 0 (IL10 protein, human)
RN  - 0 (TNFRSF18 protein, human)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Abortion, Habitual/blood/*metabolism
MH  - Adult
MH  - Biomarkers/*metabolism
MH  - CTLA-4 Antigen/blood/metabolism
MH  - Cells, Cultured
MH  - Cross-Sectional Studies
MH  - Embryo Implantation
MH  - Female
MH  - Glucocorticoid-Induced TNFR-Related Protein/blood/metabolism
MH  - Humans
MH  - Interleukin-10/blood/metabolism
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Luteal Phase/blood/immunology
MH  - Pregnancy
MH  - T-Lymphocytes, Regulatory/*metabolism
OTO - NOTNLM
OT  - CTLA-4
OT  - GITR
OT  - IL-10
OT  - unexplained recurrent spontaneous abortion
EDAT- 2015/06/04 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/06/04 06:00
PHST- 2015/06/04 06:00 [entrez]
PHST- 2015/06/04 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.3109/14767058.2015.1039507 [doi]
PST - ppublish
SO  - J Matern Fetal Neonatal Med. 2016;29(7):1175-80. doi: 
      10.3109/14767058.2015.1039507. Epub 2015 Jun 2.