PMID- 26039375
OWN - NLM
STAT- MEDLINE
DCOM- 20160222
LR  - 20231104
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 6
DP  - 2015
TI  - New Insight for the Genetic Evaluation of Resistance to Ostreid Herpesvirus 
      Infection, a Worldwide Disease, in Crassostrea gigas.
PG  - e0127917
LID - 10.1371/journal.pone.0127917 [doi]
LID - e0127917
AB  - The Pacific oyster, Crassostrea gigas, is the most important commercial oyster 
      species cultivated in the world. Meanwhile, the ostreid herpesvirus 1 (OsHV-1) is 
      one of the major pathogens affecting the Pacific oyster, and numerous mortality 
      outbreaks related to this pathogen are now reported worldwide. To assess the 
      genetic basis of resistance to OsHV-1 infection in spat C. gigas and to 
      facilitate breeding programs for such a trait, if any exist, we compared the 
      mortality of half- and full-sib families using three field methods and a 
      controlled challenge by OsHV-1 in the laboratory. In the field, three methods 
      were tested: (A) one family per bag; (B) one family per small soft mesh bag and 
      all families inside one bag; (C) same as the previous methods but the oysters 
      were individually labelled and then mixed. The mean mortality ranged from 80 to 
      82% and was related to OsHV-1 based on viral DNA detection. The narrow-sense 
      heritability for mortality, and thus OsHV-1 resistance, ranged from 0.49 to 0.60. 
      The high positive genetic correlations across the field methods suggested no 
      genotype by environment interaction. Ideally, selective breeding could use method 
      B, which is less time- and space-consuming. The narrow sense heritability for 
      mortality under OsHV-1 challenge was 0.61, and genetic correlation between the 
      field and the laboratory was ranged from 0.68 to 0.75, suggesting a weak genotype 
      by environment interaction. Thus, most of families showing the highest survival 
      performed well in field and laboratory conditions, and a similar trend was also 
      observed for families with the lowest survival. In conclusion, this is the first 
      study demonstrating a large additive genetic variation for resistance to OsHV-1 
      infection in C. gigas, regardless of the methods used, which should help in 
      selective breeding to improve resistance to viral infection in C. gigas.
FAU - Degremont, Lionel
AU  - Degremont L
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire de 
      Genetique et de Pathologie des Mollusques Marins, Avenue Mus de Loup, La 
      Tremblade, France.
FAU - Lamy, Jean-Baptiste
AU  - Lamy JB
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire de 
      Genetique et de Pathologie des Mollusques Marins, Avenue Mus de Loup, La 
      Tremblade, France.
FAU - Pepin, Jean-Francois
AU  - Pepin JF
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire 
      Environnement Ressources des Pertuis Charentais, Avenue Mus de Loup, La 
      Tremblade, France.
FAU - Travers, Marie-Agnes
AU  - Travers MA
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire de 
      Genetique et de Pathologie des Mollusques Marins, Avenue Mus de Loup, La 
      Tremblade, France.
FAU - Renault, Tristan
AU  - Renault T
AD  - Institut Francais de Recherche pour l'Exploitation de la Mer, Laboratoire de 
      Genetique et de Pathologie des Mollusques Marins, Avenue Mus de Loup, La 
      Tremblade, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150603
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animal Diseases/epidemiology/*genetics
MH  - Animals
MH  - *Crassostrea/genetics/virology
MH  - Herpesviridae/*genetics
MH  - Herpesviridae Infections/epidemiology/*genetics/*veterinary
MH  - *Models, Biological
PMC - PMC4454582
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/06/04 06:00
MHDA- 2016/02/24 06:00
PMCR- 2015/06/03
CRDT- 2015/06/04 06:00
PHST- 2014/12/01 00:00 [received]
PHST- 2015/04/20 00:00 [accepted]
PHST- 2015/06/04 06:00 [entrez]
PHST- 2015/06/04 06:00 [pubmed]
PHST- 2016/02/24 06:00 [medline]
PHST- 2015/06/03 00:00 [pmc-release]
AID - PONE-D-14-52950 [pii]
AID - 10.1371/journal.pone.0127917 [doi]
PST - epublish
SO  - PLoS One. 2015 Jun 3;10(6):e0127917. doi: 10.1371/journal.pone.0127917. 
      eCollection 2015.