PMID- 26040772
OWN - NLM
STAT- MEDLINE
DCOM- 20160428
LR  - 20220317
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Linking)
VI  - 9
IP  - 6
DP  - 2015 Jun 23
TI  - Reduced Cytotoxicity of Graphene Nanosheets Mediated by Blood-Protein Coating.
PG  - 5713-24
LID - 10.1021/nn5066606 [doi]
AB  - The advent and pending wide use of nanoscale materials urges a biosafety 
      assessment and safe design of nanomaterials that demonstrate applicability to 
      human medicine. In biological microenvironment, biomolecules will bind onto 
      nanoparticles forming corona and endow nanoparticles new biological identity. 
      Since blood-circulatory system will most likely be the first interaction organ 
      exposed to these nanomaterials, a deep understanding of the basic interaction 
      mechanisms between serum proteins and foreign nanoparticles may help to better 
      clarify the potential risks of nanomaterials and provide guidance on safe design 
      of nanomaterials. In this study, the adsorption of four high-abundance blood 
      proteins onto the carbon-based nanomaterial graphene oxide (GO) and reduced GO 
      (rGO) were investigated via experimental (AFM, florescence spectroscopy, SPR) and 
      simulation-based (molecular dynamics) approaches. Among the proteins in question, 
      we observe competitive binding to the GO surface that features a melange of 
      distinct packing modes. Our MD simulations reveal that the protein adsorption is 
      mainly enthalpically driven through strong pi-pi stacking interactions between GO 
      and aromatic protein residues, in addition to hydrophobic interactions. Overall, 
      these results were in line with previous findings related to adsorption of serum 
      proteins onto single-walled carbon nanotubes (SWCNTs), but GO exhibits a dramatic 
      enhancement of adsorption capacity compared to this one-dimensional carbon form. 
      Encouragingly, protein-coated GO resulted in a markedly less cytotoxicity than 
      pristine and protein-coated SWCNTs, suggesting a useful role for this planar 
      nanomaterial in biomedical applications.
FAU - Chong, Yu
AU  - Chong Y
AD  - daggerInstitute of Quantitative Biology and Medicine, SRMP and RAD-X, Collaborative 
      Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, 
      Soochow University, Suzhou 215123, China.
FAU - Ge, Cuicui
AU  - Ge C
AD  - daggerInstitute of Quantitative Biology and Medicine, SRMP and RAD-X, Collaborative 
      Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, 
      Soochow University, Suzhou 215123, China.
FAU - Yang, Zaixing
AU  - Yang Z
AD  - daggerInstitute of Quantitative Biology and Medicine, SRMP and RAD-X, Collaborative 
      Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, 
      Soochow University, Suzhou 215123, China.
FAU - Garate, Jose Antonio
AU  - Garate JA
AD  - double daggerIBM Thomas J. Watson Research Center, Yorktown Heights, New York 10598, United 
      States.
FAU - Gu, Zonglin
AU  - Gu Z
AD  - daggerInstitute of Quantitative Biology and Medicine, SRMP and RAD-X, Collaborative 
      Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, 
      Soochow University, Suzhou 215123, China.
FAU - Weber, Jeffrey K
AU  - Weber JK
AD  - double daggerIBM Thomas J. Watson Research Center, Yorktown Heights, New York 10598, United 
      States.
FAU - Liu, Jiajia
AU  - Liu J
AD  - daggerInstitute of Quantitative Biology and Medicine, SRMP and RAD-X, Collaborative 
      Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, 
      Soochow University, Suzhou 215123, China.
FAU - Zhou, Ruhong
AU  - Zhou R
AD  - daggerInstitute of Quantitative Biology and Medicine, SRMP and RAD-X, Collaborative 
      Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, 
      Soochow University, Suzhou 215123, China.
AD  - double daggerIBM Thomas J. Watson Research Center, Yorktown Heights, New York 10598, United 
      States.
AD  -  section signDepartment of Chemistry, Columbia University, New York, New York 10027, United 
      States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150609
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (Blood Proteins)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Adsorption
MH  - Blood Proteins/*chemistry
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Graphite/adverse effects/chemistry/*toxicity
MH  - Humans
MH  - Molecular Dynamics Simulation
MH  - Nanoparticles/adverse effects/*chemistry/*toxicity
MH  - Surface Properties
OTO - NOTNLM
OT  - blood proteins
OT  - cytotoxicity
OT  - graphene
OT  - interactions
OT  - safe design
EDAT- 2015/06/05 06:00
MHDA- 2016/04/29 06:00
CRDT- 2015/06/05 06:00
PHST- 2015/06/05 06:00 [entrez]
PHST- 2015/06/05 06:00 [pubmed]
PHST- 2016/04/29 06:00 [medline]
AID - 10.1021/nn5066606 [doi]
PST - ppublish
SO  - ACS Nano. 2015 Jun 23;9(6):5713-24. doi: 10.1021/nn5066606. Epub 2015 Jun 9.