PMID- 26041412
OWN - NLM
STAT- MEDLINE
DCOM- 20160817
LR  - 20211203
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 39
IP  - 11
DP  - 2015 Nov
TI  - The mammalian target of rapamycin signaling pathway regulates myocyte enhancer 
      factor-2C phosphorylation levels through integrin-linked kinase in goat skeletal 
      muscle satellite cells.
PG  - 1264-73
LID - 10.1002/cbin.10499 [doi]
AB  - Mammalian target of rapamycin (mTOR) signaling pathway plays a key role in muscle 
      development and is involved in multiple intracellular signaling pathways. Myocyte 
      enhancer factor-2 (MEF2) regulates muscle cell proliferation and differentiation. 
      However, how the mTOR signaling pathway regulates MEF2 activity remains unclear. 
      We isolated goat skeletal muscle satellite cells (gSSCs) as model cells to 
      explore mTOR signaling pathway regulation of MEF2C. We inhibited mTOR activity in 
      gSSCs with PP242 and found that MEF2C phosphorylation was decreased and that 
      muscle creatine kinase (MCK) expression was suppressed. Subsequently, we detected 
      integrin-linked kinase (ILK) using MEF2C coimmunoprecipitation; ILK and MEF2C 
      were colocalized in the gSSCs. We found that inhibiting mTOR activity increased 
      ILK phosphorylation levels and that inhibiting ILK activity with Cpd 22 and 
      knocking down ILK with small interfering RNA increased MEF2C phosphorylation and 
      MCK expression. In the presence of Cpd 22, mTOR activity inhibition did not 
      affect MEF2C phosphorylation. Moreover, ILK dephosphorylated MEF2C in vitro. 
      These results suggest that the mTOR signaling pathway regulates MEF2C positively 
      and regulates ILK negatively and that ILK regulates MEF2C negatively. It appears 
      that the mTOR signaling pathway regulates MEF2C through ILK, further regulating 
      the expression of muscle-related genes in gSSCs.
CI  - (c) 2015 International Federation for Cell Biology.
FAU - Wu, Haiqing
AU  - Wu H
AD  - Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of 
      Education, Inner Mongolia University, China.
FAU - Ren, Yu
AU  - Ren Y
AD  - Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of 
      Education, Inner Mongolia University, China.
FAU - Pan, Wei
AU  - Pan W
AD  - Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of 
      Education, Inner Mongolia University, China.
FAU - Dong, Zhenguo
AU  - Dong Z
AD  - Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of 
      Education, Inner Mongolia University, China.
FAU - Cang, Ming
AU  - Cang M
AD  - Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of 
      Education, Inner Mongolia University, China.
FAU - Liu, Dongjun
AU  - Liu D
AD  - Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of 
      Education, Inner Mongolia University, China.
LA  - eng
PT  - Journal Article
DEP - 20150715
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (MEF2 Transcription Factors)
RN  - EC 2.7.1.- (integrin-linked kinase)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Goats
MH  - MEF2 Transcription Factors/*metabolism
MH  - Muscle, Skeletal/cytology/enzymology/metabolism
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Satellite Cells, Skeletal Muscle/enzymology/*metabolism
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - ILK
OT  - MEF2C
OT  - mTOR signaling pathway
OT  - regulation
OT  - skeletal muscle satellite cells
EDAT- 2015/06/05 06:00
MHDA- 2016/08/18 06:00
CRDT- 2015/06/05 06:00
PHST- 2015/04/29 00:00 [received]
PHST- 2015/05/30 00:00 [accepted]
PHST- 2015/06/05 06:00 [entrez]
PHST- 2015/06/05 06:00 [pubmed]
PHST- 2016/08/18 06:00 [medline]
AID - 10.1002/cbin.10499 [doi]
PST - ppublish
SO  - Cell Biol Int. 2015 Nov;39(11):1264-73. doi: 10.1002/cbin.10499. Epub 2015 Jul 
      15.