PMID- 26041558
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 45
IP  - 2
DP  - 2016 Feb
TI  - M2 macrophages and inflammatory cells in oral lesions of chronic 
      paracoccidioidomycosis.
PG  - 141-7
LID - 10.1111/jop.12333 [doi]
AB  - BACKGROUND: Paracoccidioidomycosis (PCM) is a systemic fungal infection caused by 
      Paracoccidioides brasiliensis (Pb) and associated with deficient cellular immune 
      response, which is modulated by inflammatory cells, mainly macrophages, and 
      cytokines. Recently, the comprehension of the macrophage polarization mediated by 
      Th1 and Th2 cytokines has contributed to elucidate the immune response that takes 
      part in some diseases. Thus, the aim of this study was to assess the presence of 
      Th1- and Th2-immune response and also Pb counting in oral lesions of chronic PCM. 
      METHODS: Forty-eight cases of chronic PCM oral lesions were included. All cases 
      were classified as loose or dense granulomas. S100 protein, IL-1beta, IL-6, TNF-alpha, 
      CD163 and CD68 immunoexpressions, and Pb localization were evaluated. The fungi 
      present in the tissue were quantified by anti-Pb antibody. RESULTS: Most patients 
      were white men with mean age of 47 years old and showed higher incidence of 
      multiple lesions. Loose granulomas were predominant and exhibited a great amount 
      of M2 macrophages, which were visualized with anti-CD163 antibody. The expression 
      for CD163 and CD68 was similar (P = 0.05), highlighting the predominance of M2 
      macrophages in PCM. IL-1beta, IL-6, and TNF-alpha immunoexpression did not significantly 
      change with CD163, CD68, and S100 protein. The number of fungi was significantly 
      higher in cases with intense IL-1beta immunoexpression (P = 0.003). CONCLUSIONS: 
      M2-activated macrophages were the majority among inflammatory cells in chronic 
      PCM, characterizing the action of a Th2-immune response. Nevertheless, Th1 
      cytokines were also found; mainly IL-1beta, which was associated with fungi counting 
      in oral lesions.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - de Carli, Marina Lara
AU  - de Carli ML
AD  - Department of Clinic and Surgery, School of Dentistry, Federal University of 
      Alfenas, Alfenas, Minas Gerais, Brazil.
FAU - Miyazawa, Marta
AU  - Miyazawa M
AD  - Department of Clinic and Surgery, School of Dentistry, Federal University of 
      Alfenas, Alfenas, Minas Gerais, Brazil.
FAU - Nonogaki, Suely
AU  - Nonogaki S
AD  - Pathology Center, Adolfo Lutz Institute, Sao Paulo, Sao Paulo, Brazil.
FAU - Shirata, Neuza Kasumi
AU  - Shirata NK
AD  - Pathology Center, Adolfo Lutz Institute, Sao Paulo, Sao Paulo, Brazil.
FAU - Oliveira, Denise Tostes
AU  - Oliveira DT
AD  - Department of Stomatology- Area of Pathology, Bauru Dental School, University of 
      Sao Paulo, Bauru, Sao Paulo, Brazil.
FAU - Pereira, Alessandro Antonio Costa
AU  - Pereira AA
AD  - Department of Clinic and Surgery, School of Dentistry, Federal University of 
      Alfenas, Alfenas, Minas Gerais, Brazil.
FAU - Hanemann, Joao Adolfo Costa
AU  - Hanemann JA
AD  - Department of Clinic and Surgery, School of Dentistry, Federal University of 
      Alfenas, Alfenas, Minas Gerais, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20150603
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (S100 Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD/immunology
MH  - Antigens, Differentiation, Myelomonocytic/immunology
MH  - Chronic Disease
MH  - Cytokines/immunology/metabolism
MH  - Female
MH  - Granuloma/immunology/microbiology/pathology
MH  - Humans
MH  - Interleukin-1beta/immunology
MH  - Interleukin-6/immunology
MH  - Macrophages/*immunology/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Mouth Diseases/*immunology/*microbiology/pathology
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*immunology/microbiology/pathology
MH  - Receptors, Cell Surface/immunology
MH  - S100 Proteins/immunology
MH  - Tumor Necrosis Factor-alpha/immunology
MH  - Young Adult
OTO - NOTNLM
OT  - Paracoccidioides
OT  - inflammation
OT  - macrophages
OT  - paracoccidioidomycosis
EDAT- 2015/06/05 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/06/05 06:00
PHST- 2015/05/07 00:00 [accepted]
PHST- 2015/06/05 06:00 [entrez]
PHST- 2015/06/05 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1111/jop.12333 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2016 Feb;45(2):141-7. doi: 10.1111/jop.12333. Epub 2015 Jun 3.