PMID- 26043099
OWN - NLM
STAT- MEDLINE
DCOM- 20160301
LR  - 20150605
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 882
DP  - 2015 Jul 2
TI  - Aminoglycoside analysis in food of animal origin with a zwitterionic stationary 
      phase and liquid chromatography-tandem mass spectrometry.
PG  - 127-39
LID - S0003-2670(15)00441-9 [pii]
LID - 10.1016/j.aca.2015.03.050 [doi]
AB  - In this study, fourteen highly polar aminoglycoside (AGs) antibiotics were 
      selected. Various stationary phases were tested, including Obelisc R, ZIC-HILIC, 
      BEH amide and aminopropyl. The nature of the stationary phase, mobile phase 
      (water or buffer solutions and acetonitrile), pH (percentage of formic acid), 
      gradient conditions and injection solvents were systematically studied as 
      relevant parameters for tuning retention selectivity and detectability of AGs in 
      liquid chromatography electrospray tandem mass spectrometry (LC-(ESI)-MS/MS). 
      Only the two zwitterionic phases (Obelisc R and ZIC-HILIC) achieved a proper 
      chromatographic separation considering interferences due to the crosstalk effect 
      in low resolution mass spectrometers. The water/acetonitrile mobile phase 
      containing 1% formic acid used with Obelisc R provided more sensitivity than the 
      highly concentrated buffered mobile phases required for ZIC-HILIC. A solid phase 
      extraction (SPE) clean-up procedure with polymeric weak cation exchange (WCX) 
      cartridges was optimized for honey, milk and liver samples. Different brands of 
      cartridges and elution solvents were tested, and the Taurus WCX offered the best 
      recovery rate with a buffer elution at pH 3. The final optimized method was 
      validated in these matrices according to Decision 2002/657/EC. A monitoring 
      campaign for sixty honey, milk and liver samples was carried out at the Food 
      Authority Control in Geneva. The concentration of dihydrostreptomycin (DSTP) 
      found in one ovine liver exceeded the established maximum residue levels (MRLs) 
      within the European and Swiss legislations but it was compliant taking into 
      account the validation data.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Diez, Cristina
AU  - Diez C
AD  - Official Food Authority Control and Veterinary Affairs (SCAV), Quai 
      Ernest-Ansermet 22, 1211 Geneva 4, Switzerland; School of Pharmaceutical 
      Sciences, University of Lausanne, University of Geneva, Boulevard d'Yvoy 20, 1211 
      Geneva 4, Switzerland.
FAU - Guillarme, Davy
AU  - Guillarme D
AD  - School of Pharmaceutical Sciences, University of Lausanne, University of Geneva, 
      Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.
FAU - Staub Sporri, Aline
AU  - Staub Sporri A
AD  - Official Food Authority Control and Veterinary Affairs (SCAV), Quai 
      Ernest-Ansermet 22, 1211 Geneva 4, Switzerland.
FAU - Cognard, Emmanuelle
AU  - Cognard E
AD  - Official Food Authority Control and Veterinary Affairs (SCAV), Quai 
      Ernest-Ansermet 22, 1211 Geneva 4, Switzerland.
FAU - Ortelli, Didier
AU  - Ortelli D
AD  - Official Food Authority Control and Veterinary Affairs (SCAV), Quai 
      Ernest-Ansermet 22, 1211 Geneva 4, Switzerland.
FAU - Edder, Patrick
AU  - Edder P
AD  - Official Food Authority Control and Veterinary Affairs (SCAV), Quai 
      Ernest-Ansermet 22, 1211 Geneva 4, Switzerland.
FAU - Rudaz, Serge
AU  - Rudaz S
AD  - School of Pharmaceutical Sciences, University of Lausanne, University of Geneva, 
      Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland. Electronic address: 
      serge.rudaz@unige.ch.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150331
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Aminoglycosides)
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Aminoglycosides/*analysis
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, Liquid/*methods
MH  - Food Contamination/*analysis
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Aminoglycosides
OT  - Honey
OT  - Liver
OT  - Milk
OT  - Zwitterionic
EDAT- 2015/06/05 06:00
MHDA- 2016/03/02 06:00
CRDT- 2015/06/05 06:00
PHST- 2015/02/16 00:00 [received]
PHST- 2015/03/26 00:00 [revised]
PHST- 2015/03/28 00:00 [accepted]
PHST- 2015/06/05 06:00 [entrez]
PHST- 2015/06/05 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
AID - S0003-2670(15)00441-9 [pii]
AID - 10.1016/j.aca.2015.03.050 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2015 Jul 2;882:127-39. doi: 10.1016/j.aca.2015.03.050. Epub 2015 
      Mar 31.