PMID- 26044082
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20220331
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 25
IP  - 2
DP  - 2016
TI  - Characterization of HLA-G and Related Immunosuppressive Effects in Human 
      Umbilical Cord Stroma-Derived Stem Cells.
PG  - 217-28
LID - 10.3727/096368915X688182 [doi]
AB  - Mesenchymal stem cells (MSCs) and especially those derived from fetal tissues 
      exert a potent immunosuppressive effect that can be enhanced under inflammatory 
      conditions. This study aimed to explore the immunosuppressive properties of human 
      umbilical cord mesenchymal stem cells (HUCMSCs). We found that HLA-G, the 
      nonclassical HLA allele with strong immune-inhibitory properties, was much more 
      expressed on the HUCMSCs than on MSCs of other origins. Flow cytometry revealed 
      that 90.8% of the HUCMSCs expressed HLA-G. RT-PCR revealed expression of HLA-G1, 
      HLA-G5, and HLA-G7 in all of four HUCMSC lines. In a mixed lymphocyte reaction 
      assay, the HUCMSCs inhibited the proliferation of lymphocytes by 35 +/- 3% and 
      could be reversed by treatment with an HLA-G blocking antibody. Upon coculture 
      with the HUCMSCs, peripheral blood mononuclear cells expressed lower levels of 
      proinflammatory mediators such as IL-6, TNF-alpha, and VEGF-alpha. This immunosuppressive 
      effect was enhanced when the HUCMSCs were pretreated with IFN-gamma, such that the 
      expression of HLA-G was highly activated and HLA-DR diminished. The same 
      phenomenon was not observed in MSCs derived from bone marrow or the placenta. In 
      a xenograft rejection assay, the HUCMSCs survived in immunocompetent mice, 
      whereas primary fibroblasts did not survive. This study confirms the 
      HLA-G-related immunosuppressive property of HUCMSCs, which is more potent than 
      MSCs of other origin. A good tolerance of this mesenchymal stem cell in 
      allogeneic transplantation can thus be anticipated.
FAU - Ding, Dah-Ching
AU  - Ding DC
AD  - Department of Obstetrics and Gynecology, Buddhist Tzu-Chi Buddhist General 
      Hospital, Hualien, Taiwan.
FAU - Chou, Hsiang-Lan
AU  - Chou HL
FAU - Chang, Yu-Hsun
AU  - Chang YH
FAU - Hung, Wei-Ting
AU  - Hung WT
FAU - Liu, Hwan-Wun
AU  - Liu HW
FAU - Chu, Tang-Yuan
AU  - Chu TY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150603
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (HLA-G Antigens)
SB  - IM
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation/*physiology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Female
MH  - HLA-G Antigens/*immunology
MH  - Humans
MH  - Immune Tolerance/immunology
MH  - Leukocytes, Mononuclear/immunology
MH  - Lymphocyte Culture Test, Mixed/methods
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology/*immunology
MH  - Pregnancy
MH  - Umbilical Cord/*cytology
EDAT- 2015/06/06 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/06/06 06:00
PHST- 2015/06/06 06:00 [entrez]
PHST- 2015/06/06 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - content-CT-1292_Ding_et_al [pii]
AID - 10.3727/096368915X688182 [doi]
PST - ppublish
SO  - Cell Transplant. 2016;25(2):217-28. doi: 10.3727/096368915X688182. Epub 2015 Jun 
      3.