PMID- 26044185
OWN - NLM
STAT- MEDLINE
DCOM- 20180409
LR  - 20181202
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 28
IP  - 12
DP  - 2016 Oct
TI  - Androgen and oestrogen modulation by D-aspartate in rat epididymis.
PG  - 1865-1872
LID - 10.1071/RD15092 [doi]
AB  - Testosterone (T) synthesised in Leydig cells enters the epididymis and may there 
      be converted into dihydrotestosterone (DHT) by 5alpha-reductase (5alpha-red) or into 
      17beta-oestradiol (E2) by P450 aromatase (P450-aro). D-aspartate (D-Asp) is known to 
      induce T synthesis in the testis. In this study, we investigated the effects of 
      in vivo D-Asp administration in two major regions of the rat epididymis (Region 
      I: initial segment, caput, corpus; Region II: cauda). The results suggest that 
      exogenous D-Asp was taken up by both regions of rat epididymis. D-Asp 
      administration induced a rapid increase in T, followed by a more gradual decrease 
      in the T:DHT ratio in Region I. In Region II, T levels rapidly decreased and the 
      T:DHT ratio was consistently lower relative to the control. Expression of 5alpha-red 
      and androgen receptor genes showed a good correlation with DHT levels in both 
      regions. D-Asp treatment also induced an increase of both E2 levels and 
      oestradiol receptor-alpha (ERalpha) expression in Region I, whereas neither E2 levels nor 
      ERalpha expression were affected in Region II. The early increase of P450-aro 
      expression in Region I and late increase in Region II suggests a direct 
      involvement of D-Asp modulation in P450-aro gene expression. Our results suggest 
      that D-Asp modulates androgen and oestrogen levels and expression of androgen and 
      oestrogen receptors in the rat epididymis by acting on the expression of 5alpha-red 
      and P450-aro genes.
FAU - Falvo, S
AU  - Falvo S
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Via Vivaldi 43, 81100, Caserta, Italy.
FAU - Di Fiore, M M
AU  - Di Fiore MM
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Via Vivaldi 43, 81100, Caserta, Italy.
FAU - Burrone, L
AU  - Burrone L
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Via Vivaldi 43, 81100, Caserta, Italy.
FAU - Baccari, G Chieffi
AU  - Baccari GC
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Via Vivaldi 43, 81100, Caserta, Italy.
FAU - Longobardi, S
AU  - Longobardi S
AD  - Medical Liaison Office, Merck Serono S.p.A., Via Casilina 125, 00176, Roma, 
      Italy.
FAU - Santillo, A
AU  - Santillo A
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Seconda Universita di Napoli, Via Vivaldi 43, 81100, Caserta, Italy.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Estrogens)
RN  - 0 (Receptors, Androgen)
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 3XMK78S47O (Testosterone)
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.14.14.1 (Aromatase)
RN  - EC 1.3.99.5 (3-Oxo-5-alpha-Steroid 4-Dehydrogenase)
SB  - IM
MH  - 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/metabolism
MH  - Animals
MH  - Aromatase/metabolism
MH  - D-Aspartic Acid/*pharmacology
MH  - Dihydrotestosterone/*metabolism
MH  - Epididymis/*drug effects/physiology
MH  - Estradiol/metabolism
MH  - Estrogens/*metabolism
MH  - Male
MH  - Rats
MH  - Receptors, Androgen/metabolism
MH  - Testosterone/*metabolism
EDAT- 2015/06/06 06:00
MHDA- 2018/04/10 06:00
CRDT- 2015/06/06 06:00
PHST- 2015/03/08 00:00 [received]
PHST- 2015/05/05 00:00 [accepted]
PHST- 2015/06/06 06:00 [pubmed]
PHST- 2018/04/10 06:00 [medline]
PHST- 2015/06/06 06:00 [entrez]
AID - RD15092 [pii]
AID - 10.1071/RD15092 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2016 Oct;28(12):1865-1872. doi: 10.1071/RD15092.