PMID- 26044349 OWN - NLM STAT- MEDLINE DCOM- 20160706 LR - 20181202 IS - 1878-1705 (Electronic) IS - 1567-5769 (Linking) VI - 28 IP - 1 DP - 2015 Sep TI - Resveratrol suppresses persistent airway inflammation and hyperresponsivess might partially via nerve growth factor in respiratory syncytial virus-infected mice. PG - 121-8 LID - S1567-5769(15)00260-X [pii] LID - 10.1016/j.intimp.2015.05.031 [doi] AB - Respiratory syncytial virus (RSV) infection is involved in persistent and recurrent wheezing. There are no effective and safe drugs for the sequelae of persistent wheezing after early bronchiolitis. In this study, we investigated the effect of resveratrol on persistent airway inflammation and airway hyperresponsiveness (AHR) induced by RSV infection. RSV-infected mice were sacrificed at serial time points after infection to collect samples and measure the number of inflammatory cells and levels of interferon-gamma (IFN-gamma), nerve growth factor (NGF), and brain-derived neurotrophic factor (BDNF). Airway inflammation, AHR, and the levels of NGF and BDNF were detected after resveratrol administration. Furthermore, Ab-NGF was used to investigate the role of NGF in RSV-induced prolonged airway inflammation and AHR. We found that RSV RNA remained detectable in the lungs of RSV-infected mice on day 60, accompanying persistent airway inflammation and AHR for 60 days. IFN-gamma levels in BALF were increased on day 7 but reduced to normal levels by day 14 post-RSV infection, while NGF and BDNF levels gradually increased from day 14 to 60. Furthermore, after resveratrol treatment, the total number of cells in BALF was reduced; the number of inflammatory cells infiltrating the lungs was also lower. Resveratrol attenuated the airway response to methacholine and significantly decreased NGF levels in BALF without affecting BDNF levels. Moreover, airway inflammation and AHR associated with RSV persistence were attenuated after Ab-NGF administration. In all, resveratrol suppresses persistent airway inflammation and AHR might partially through reducing the levels of NGF after RSV infection. CI - Copyright (c) 2015 Elsevier B.V. All rights reserved. FAU - Zang, Na AU - Zang N AD - Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China; Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, China. FAU - Li, Simin AU - Li S AD - Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China. FAU - Li, Wei AU - Li W AD - Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China; Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, China. FAU - Xie, Xiaohong AU - Xie X AD - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, China. FAU - Ren, Luo AU - Ren L AD - Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China. FAU - Long, Xiaoru AU - Long X AD - Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China; Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, China. FAU - Xie, Jun AU - Xie J AD - Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China. FAU - Deng, Yu AU - Deng Y AD - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, China. FAU - Fu, Zhou AU - Fu Z AD - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, China. FAU - Xu, Fadi AU - Xu F AD - Pathophysiology Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA. FAU - Liu, Enmei AU - Liu E AD - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, China. Electronic address: emliu186@hotmail.com. LA - eng GR - R01HL119683-01/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20150601 PL - Netherlands TA - Int Immunopharmacol JT - International immunopharmacology JID - 100965259 RN - 0 (Anti-Inflammatory Agents, Non-Steroidal) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Viral) RN - 0 (Stilbenes) RN - 82115-62-6 (Interferon-gamma) RN - 9061-61-4 (Nerve Growth Factor) RN - Q369O8926L (Resveratrol) SB - IM MH - Animals MH - Anti-Inflammatory Agents, Non-Steroidal/*therapeutic use MH - Brain-Derived Neurotrophic Factor/metabolism MH - Bronchoalveolar Lavage Fluid/chemistry/cytology MH - Female MH - Interferon-gamma/metabolism MH - Lung/pathology/virology MH - Mice MH - Mice, Inbred BALB C MH - Nerve Growth Factor/*drug effects MH - Pneumonia/*drug therapy MH - RNA, Viral/analysis MH - Respiratory Function Tests MH - Respiratory Hypersensitivity/*drug therapy MH - Respiratory Syncytial Virus Infections/*drug therapy/pathology/virology MH - Respiratory Syncytial Viruses MH - Resveratrol MH - Stilbenes/*therapeutic use OTO - NOTNLM OT - NGF OT - Persistent AHR OT - Persistent airway inflammation OT - RSV OT - Resveratrol EDAT- 2015/06/06 06:00 MHDA- 2016/07/07 06:00 CRDT- 2015/06/06 06:00 PHST- 2014/12/07 00:00 [received] PHST- 2015/05/04 00:00 [revised] PHST- 2015/05/19 00:00 [accepted] PHST- 2015/06/06 06:00 [entrez] PHST- 2015/06/06 06:00 [pubmed] PHST- 2016/07/07 06:00 [medline] AID - S1567-5769(15)00260-X [pii] AID - 10.1016/j.intimp.2015.05.031 [doi] PST - ppublish SO - Int Immunopharmacol. 2015 Sep;28(1):121-8. doi: 10.1016/j.intimp.2015.05.031. Epub 2015 Jun 1.