PMID- 26044732
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160726
LR  - 20181113
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 68
IP  - 4
DP  - 2016 Aug
TI  - Inhibitor of p53-p21 pathway induces the differentiation of human umbilical cord 
      derived mesenchymal stem cells into cardiomyogenic cells.
PG  - 1257-65
LID - 10.1007/s10616-015-9886-5 [doi]
AB  - P53 is shown recently to play an important role in the proliferation and 
      differentiation of mesenchymal stem cells. In this study, human umbilical cord 
      derived mesenchymal stem cells (hUCMSCs) were isolated and purified from the 
      umbilical cords of normal or cesarean term deliveries, after treatment with 
      20 mumol/L PFT-alpha for 24 h, hUCMSCs were continued to be cultured for 4 weeks, 
      cardiac-specific protein expression of cTnI, Desmin and Nkx2.5 was determined 
      using immunofluorescence assay and RT-PCR. The expression of p53 and p21 was 
      detected by western blot. Results showed that no expression of cTnI, Desmin or 
      Nkx2.5 was observed in the control and the PFT-alpha group at 1 week after induction. 
      However, after 4 weeks, while control group still had little expression of cTnI, 
      Desmin and Nkx2.5, the PFT-alpha group demonstrated strong expression of cTnI, Desmin 
      and Nkx2.5 (P < 0.001). At 4 weeks after induction, differentiation rate of 
      cardiomyogenic cells in the PFT-alpha group (36.98 %) was significantly higher than 
      that in the control group (4.41 %) (P < 0.01). Western blot analysis show that 
      downregulation of p53 and p21 was seen in the PFT-alpha group at 4 weeks. The 
      difference compared with the control group was statistically significant 
      (P < 0.01). In conclusion, PFT-alpha can promote the differentiation of hUCMSCs into 
      cardiomyogenic cells by modulating the p53-p21 pathway.
FAU - Ruan, Zhong-Bao
AU  - Ruan ZB
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, 225300, People's 
      Republic of China.
FAU - Zhu, Li
AU  - Zhu L
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, 225300, People's 
      Republic of China. tzcardiac@163.com.
FAU - Yin, Yi-Gang
AU  - Yin YG
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, 225300, People's 
      Republic of China.
FAU - Chen, Ge-Cai
AU  - Chen GC
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, 225300, People's 
      Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20150605
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC4960174
OTO - NOTNLM
OT  - Cardiomyogenic cells
OT  - Differentiation
OT  - Human umbilical cord derived mesenchymal stem cells
OT  - PFT-alpha
EDAT- 2015/06/06 06:00
MHDA- 2015/06/06 06:01
PMCR- 2017/08/01
CRDT- 2015/06/06 06:00
PHST- 2014/06/01 00:00 [received]
PHST- 2015/05/08 00:00 [accepted]
PHST- 2015/06/06 06:00 [entrez]
PHST- 2015/06/06 06:00 [pubmed]
PHST- 2015/06/06 06:01 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - 10.1007/s10616-015-9886-5 [pii]
AID - 9886 [pii]
AID - 10.1007/s10616-015-9886-5 [doi]
PST - ppublish
SO  - Cytotechnology. 2016 Aug;68(4):1257-65. doi: 10.1007/s10616-015-9886-5. Epub 2015 
      Jun 5.