PMID- 26045773
OWN - NLM
STAT- MEDLINE
DCOM- 20160322
LR  - 20181113
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 8
IP  - 3
DP  - 2015
TI  - Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate 
      deposition and renal oxidative stress in mice.
PG  - 2680-9
AB  - The aim of this study is to evaluate the protective effect and underlying 
      mechanism of hydrogen gas (H2) to glyoxylate induced renal calcium oxalate (CaOx) 
      crystal deposition in mice. In present work, rodent renal CaOx crystal deposition 
      model was introduced by intra-abdominal injection of glyoxylate (100 mg/kg/d) for 
      5 days. Two days before administration of glyoxylate, inhalation of H2 for 30 min 
      per day was initiated and continued for 7 days. By the end of the study, the 
      samples of 24 hours urine, serum and renal tissue were collected for biochemical 
      and pathological assay. According to levels of urine calcium excretion, renal 
      calcium deposition, a serum excretion of kidney injury molecule-1 (KIM-1) assay 
      and a TUNEL assay, inhalation of H2 could successfully decrease the CaOx 
      crystallizations and protect against renal injury. Crystal deposition in the 
      kidneys is associated with oxidative stress, which was indicated by increased 
      levels of renal malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) and 
      decreased activities of superoxide dismutase (SOD), glutathione (GSH) and 
      catalase (CAT). These effects were reversed by a high-dose H2 pretreatment. The 
      renal expressions of osteopontin (OPN), CD44, monocyte chemoattractant protein-1 
      (MCP-1) and interleukin-10 (IL-10) were markedly increased in glyoxylate-treated 
      mice, and H2 significantly attenuated the increase of OPN, CD44 and MCP-1 but 
      upregulated the expression of IL-10. Our findings demonstrate that inhalation of 
      H2 reduces renal crystallization, renal oxidative injury and inflammation and it 
      may be a candidate agent with few adverse effects for prevention of 
      nephrolithiasis.
FAU - Peng, Zhongjiang
AU  - Peng Z
AD  - Department of Nephrology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
FAU - Chen, Wei
AU  - Chen W
AD  - Department of Nephrology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
FAU - Wang, Li
AU  - Wang L
AD  - Department of Nephrology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
FAU - Ye, Zhouheng
AU  - Ye Z
AD  - Department of Aviation Medicine, Faculty of Naval Medicine, Second Military 
      Medical University Shanghai, China.
FAU - Gao, Songyan
AU  - Gao S
AD  - School of Pharmacy, Second Military Medical University Shanghai, China.
FAU - Sun, Xuejun
AU  - Sun X
AD  - Department of Aviation Medicine, Faculty of Naval Medicine, Second Military 
      Medical University Shanghai, China.
FAU - Guo, Zhiyong
AU  - Guo Z
AD  - Department of Nephrology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150301
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Glyoxylates)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - JQ39C92HH6 (glyoxylic acid)
RN  - Nephrolithiasis, Calcium Oxalate
SB  - IM
MH  - Administration, Inhalation
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Disease Models, Animal
MH  - Glyoxylates/toxicity
MH  - Hydrogen/*administration & dosage
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Kidney/*drug effects/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nephrolithiasis/*prevention & control
MH  - Oxidative Stress/*drug effects
PMC - PMC4440082
OTO - NOTNLM
OT  - Hydrogen gas
OT  - calcium oxalate
OT  - glyoxylate
OT  - kidney injury molecule-1
OT  - oxidative stress
EDAT- 2015/06/06 06:00
MHDA- 2016/03/24 06:00
PMCR- 2015/03/01
CRDT- 2015/06/06 06:00
PHST- 2015/01/04 00:00 [received]
PHST- 2015/02/26 00:00 [accepted]
PHST- 2015/06/06 06:00 [entrez]
PHST- 2015/06/06 06:00 [pubmed]
PHST- 2016/03/24 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2015 Mar 1;8(3):2680-9. eCollection 2015.