PMID- 26045902
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150605
LR  - 20201001
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 7
IP  - 3
DP  - 2015
TI  - BCR-ABL1 and CD66c exhibit high concordance in minimal residual disease detection 
      of adult B-acute lymphoblastic leukemia.
PG  - 632-9
AB  - OBJECTIVE: To investigate the relationship between surface expression of CD66c 
      and the breakpoint cluster region-Abelson (BCR-ABL1) fusion gene in B-acute 
      lymphoblastic leukemia (B-ALL) at primary diagnosis, and their concordance during 
      minimal residual disease (MRD) monitoring. METHODS: Bone marrow biopsies were 
      collected from newly diagnosed B-ALL patients (n = 43) between September 2011 and 
      September 2014. Karyotyping was used to detect Philadelphia chromosome (Ph), and 
      fluorescence in situ hybridization (FISH) and reverse transcription-polymerase 
      chain reaction (RT-PCR) were used to detect BCR-ABL1 fusion gene. 
      Immunophenotyping was performed by flow cytometry for leukemia. Patients with 
      both CD66c expression and BCR-ABL1 were further assessed for MRD during 
      treatment. RESULTS: Overall, 26/43 (60.5%) B-ALL patients were positive for 
      BCR-ABL1 fusion gene expression, and all Ph positive cases (17/43; 39.5%) 
      expressed BCR-ABL1 and CD66c. CD66c was expressed at significantly higher levels 
      in BCR-ABL1 positive than negative patients (24/26, 92.3% vs. 11/17, 64.7%; P = 
      0.042), and furthermore, in all Ph positive cases (17/17, 100% vs. 18/26, 69.2%; 
      P = 0.014). When BCR-ABL1 was set as the gold standard for the presence or 
      absence of MRD after treatment, both CD66c alone and the MRD panel including 
      CD66c demonstrated high diagnostic performance for the detection of MRD, with 
      values of area under the receptor operation curve (ROC) of 0.881 vs. 0.891 
      respectively. CONCLUSIONS: The stable expression pattern of CD66c has noteworthy 
      clinical value in B-ALL not only in the recognition of abnormal leukemia cells at 
      primary diagnosis but also in monitoring of MRD during the treatment, especially 
      in patients without definitely cytogenetic or molecular abnormal, and thus, 
      warrants further investigation as a routine clinical marker for MRD detection by 
      flow cytometry.
FAU - Tang, Gu-Sheng
AU  - Tang GS
AD  - Department of Hematology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
FAU - Wu, Jun
AU  - Wu J
AD  - Department of Sterile Supply, Changhai Hospital, Second Military Medical 
      University Shanghai, China.
FAU - Liu, Min
AU  - Liu M
AD  - Department of Hematology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
FAU - Chen, Hui
AU  - Chen H
AD  - Department of Hematology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
FAU - Gong, Shen-Glan
AU  - Gong SG
AD  - Department of Hematology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
FAU - Yang, Jian-Min
AU  - Yang JM
AD  - Department of Hematology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
FAU - Hu, Xiao-Xia
AU  - Hu XX
AD  - Department of Hematology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
FAU - Wang, Jian-Min
AU  - Wang JM
AD  - Department of Hematology, Changhai Hospital, Second Military Medical University 
      Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20150315
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC4448202
OTO - NOTNLM
OT  - B-acute lymphoblastic leukemia (B-ALL)
OT  - BCR-ABL1
OT  - CD66c
OT  - diagnostic performance
OT  - minimal residual disease (MRD)
EDAT- 2015/06/06 06:00
MHDA- 2015/06/06 06:01
PMCR- 2015/03/15
CRDT- 2015/06/06 06:00
PHST- 2014/12/12 00:00 [received]
PHST- 2015/02/10 00:00 [accepted]
PHST- 2015/06/06 06:00 [entrez]
PHST- 2015/06/06 06:00 [pubmed]
PHST- 2015/06/06 06:01 [medline]
PHST- 2015/03/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2015 Mar 15;7(3):632-9. eCollection 2015.