PMID- 26046494
OWN - NLM
STAT- MEDLINE
DCOM- 20160324
LR  - 20191210
IS  - 1946-6544 (Electronic)
IS  - 1946-6536 (Linking)
VI  - 26
IP  - 3
DP  - 2015 Jun
TI  - Murine Bone Marrow-Derived Dendritic Cells Transduced by Light-Helper-Dependent 
      Herpes Simplex Virus-1 Amplicon Vector Acquire a Mature Dendritic Cell Phenotype.
PG  - 93-102
LID - 10.1089/hgtb.2015.042 [doi]
AB  - Dendritic cells (DCs) turn into the most potent antigen-presenting cells 
      following a complex transforming process, which leads to their maturation. Herpes 
      simplex virus-1 (HSV-1) amplicon vectors represent highly versatile viral vector 
      platforms with the ability to transduce immature DCs at exceedingly high 
      efficiencies, while the efficiency of infection of mature DCs is significantly 
      low. However, the bacterial artificial chromosome (BAC)-dependent (BD) amplicon 
      vectors tested so far do not result in the maturation of mouse bone 
      marrow-derived DCs (BMDCs) in vitro. In this study we investigated the effects of 
      light-helper-dependent (LHD) amplicon vectors produced with the 
      replication-defective HSV-1 LaLDeltaJ helper virus system. First, we observed that 
      transgene expression in BMDC cultures was equally potent between the LHD and the 
      BD amplicon vectors. We determined that the percentage of transduced cells and 
      the duration of transgene expression were negatively influenced by the presence 
      of increasing levels of helper virus. Second, infection by the LHD amplicon 
      vector as well as the helper HSV-1 LaLDeltaJ virus alone resulted in the phenotypic 
      maturation of BMDCs and the expression of both interferon-stimulated genes and 
      proinflammatory cytokines. Further comparisons of the gene expression of infected 
      DCs showed that while interferon-stimulated genes such as Ifit1, Ifit3, Mx2, 
      Isg15, and Cxcl10 were induced by both BD and LHD amplicon vectors, early 
      proinflammatory cytokine gene expression (Tnfa, Il1a, Il1b, Il6, Il10, Il12b, 
      Cxcl1, and Cxcl16) and DC maturation were mediated only by the LHD amplicons.
FAU - Oz-Arslan, Devrim
AU  - Oz-Arslan D
AD  - 1 Molecular Virology Laboratory, Hellenic Pasteur Institute , 11521 Athens, 
      Greece .
AD  - 2 Department of Biophysics, School of Medicine, Acibadem University , 34848 
      Istanbul, Turkey .
FAU - Tsitoura, Eliza
AU  - Tsitoura E
AD  - 1 Molecular Virology Laboratory, Hellenic Pasteur Institute , 11521 Athens, 
      Greece .
AD  - 3 Laboratory of Cellular and Molecular Pneumonology, Medical School, University 
      of Crete , 70013 Crete, Greece .
FAU - Kazazi, Dorothea
AU  - Kazazi D
AD  - 1 Molecular Virology Laboratory, Hellenic Pasteur Institute , 11521 Athens, 
      Greece .
FAU - Kouvatsis, Vlasis
AU  - Kouvatsis V
AD  - 1 Molecular Virology Laboratory, Hellenic Pasteur Institute , 11521 Athens, 
      Greece .
FAU - Epstein, Alberto L
AU  - Epstein AL
AD  - 4 UMR 1179 INSERM-UVSQ-End-icap, Handicap Neuromusculaire, Biotherapie et 
      Pharmacologie appliquees, UFR des sciences de la sante "Simone Veil," Universite 
      de Versailles-Saint Quentin en Yvelines , 78180 Montigny-le-Bretonneux, France .
FAU - Mavromara, Penelope
AU  - Mavromara P
AD  - 1 Molecular Virology Laboratory, Hellenic Pasteur Institute , 11521 Athens, 
      Greece .
AD  - 5 Department of Molecular Biology and Genetics, Democritus University of Thrace , 
      68100 Alexandroupolis, Greece .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Gene Ther Methods
JT  - Human gene therapy methods
JID - 101573202
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Chlorocebus aethiops
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Cytokines/genetics/metabolism
MH  - Dendritic Cells/*cytology/metabolism
MH  - Genetic Vectors/genetics
MH  - Helper Viruses/*genetics
MH  - Herpesvirus 1, Human/*genetics
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phenotype
MH  - Transduction, Genetic
MH  - Vero Cells
EDAT- 2015/06/06 06:00
MHDA- 2016/03/25 06:00
CRDT- 2015/06/06 06:00
PHST- 2015/06/06 06:00 [entrez]
PHST- 2015/06/06 06:00 [pubmed]
PHST- 2016/03/25 06:00 [medline]
AID - 10.1089/hgtb.2015.042 [doi]
PST - ppublish
SO  - Hum Gene Ther Methods. 2015 Jun;26(3):93-102. doi: 10.1089/hgtb.2015.042.