PMID- 26047930
OWN - NLM
STAT- MEDLINE
DCOM- 20160513
LR  - 20150814
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 176
IP  - 7
DP  - 2015 Aug
TI  - Influence of Altered NADH Metabolic Pathway on the Respiratory-deficient Mutant 
      of Rhizopus oryzae and its L-lactate Production.
PG  - 2053-64
LID - 10.1007/s12010-015-1700-5 [doi]
AB  - Respiratory-deficient mutants of Rhizopus oryzae (R. oryzae) AS 3.3461 were 
      acquired by ultraviolet (UV) irradiation to investigate changes in intracellular 
      NADH metabolic pathway and its influence on the fermentation characteristics of 
      the strain. Compared with R. oryzae AS 3.3461, the intracellular ATP level of the 
      respiratory-deficient strain UV-1 decreased by 52.7 % and the glucose utilization 
      rate rose by 8.9 %; When incubated for 36 h, the activities of 
      phosphofructokinase (PFK), hexokinase (HK), and pyruvate kinase (PK) in the 
      mutant rose by 74.2, 7.2, and 12.0 %, respectively; when incubated for 48 h, the 
      intracellular NADH/NAD(+) ratio of the mutant rose by 14.6 %; when a mixed carbon 
      source with a glucose/gluconic acid ratio of 1:1 was substituted to culture the 
      mutant, the NADH/NAD(+) ratio decreased by 4.6 %; the ATP content dropped by 27.6 
      %; the lactate dehydrogenase (LDH) activity rose by 22.7 %; and the lactate yield 
      rose by 11.6 %. These results indicated that changes to the NADH metabolic 
      pathway under a low-energy charge level can effectively increase the glycolytic 
      rate and further improve the yield of L-lactate of R. oryzae.
FAU - Shu, Chang
AU  - Shu C
AD  - School of Biotechnology and Food Engineering, Hefei University of Technology, 193 
      Tunxi road, Hefei, 230009, People's Republic of China.
FAU - Guo, Chenchen
AU  - Guo C
FAU - Luo, Shuizhong
AU  - Luo S
FAU - Jiang, Shaotong
AU  - Jiang S
FAU - Zheng, Zhi
AU  - Zheng Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150606
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Gluconates)
RN  - 0U46U6E8UK (NAD)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - R4R8J0Q44B (gluconic acid)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Energy Metabolism/drug effects/radiation effects
MH  - Fermentation/drug effects/radiation effects
MH  - Gluconates/pharmacology
MH  - Glycolysis/drug effects/radiation effects
MH  - Intracellular Space/drug effects/metabolism/radiation effects
MH  - Lactic Acid/*biosynthesis
MH  - Mutagenesis/radiation effects
MH  - *Mutation/radiation effects
MH  - NAD/*metabolism
MH  - Oxidation-Reduction/drug effects/radiation effects
MH  - Rhizopus/*cytology/genetics/*metabolism
MH  - Ultraviolet Rays/adverse effects
EDAT- 2015/06/07 06:00
MHDA- 2016/05/14 06:00
CRDT- 2015/06/07 06:00
PHST- 2015/03/11 00:00 [received]
PHST- 2015/05/28 00:00 [accepted]
PHST- 2015/06/07 06:00 [entrez]
PHST- 2015/06/07 06:00 [pubmed]
PHST- 2016/05/14 06:00 [medline]
AID - 10.1007/s12010-015-1700-5 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2015 Aug;176(7):2053-64. doi: 10.1007/s12010-015-1700-5. 
      Epub 2015 Jun 6.