PMID- 26048259
OWN - NLM
STAT- MEDLINE
DCOM- 20161024
LR  - 20161230
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Linking)
VI  - 363
IP  - 2
DP  - 2016 Feb
TI  - Heterogeneous vascular permeability and alternative diffusion barrier in sensory 
      circumventricular organs of adult mouse brain.
PG  - 497-511
LID - 10.1007/s00441-015-2207-7 [doi]
AB  - Fenestrated capillaries of the sensory circumventricular organs (CVOs), including 
      the organum vasculosum of the lamina terminalis, the subfornical organ and the 
      area postrema, lack completeness of the blood-brain barrier (BBB) to sense a 
      variety of blood-derived molecules and to convey the information into other brain 
      regions. We examine the vascular permeability of blood-derived molecules and the 
      expression of tight-junction proteins in sensory CVOs. The present tracer assays 
      revealed that blood-derived dextran 10 k (Dex10k) having a molecular weight (MW) 
      of 10,000 remained in the perivascular space between the inner and outer basement 
      membranes, but fluorescein isothiocyanate (FITC; MW: 389) and Dex3k (MW: 3000) 
      diffused into the parenchyma. The vascular permeability of FITC was higher at 
      central subdivisions than at distal subdivisions. Neither FITC nor Dex3k diffused 
      beyond the dense network of glial fibrillar acidic protein (GFAP)-positive 
      astrocytes/tanycytes. The expression of tight-junction proteins such as occludin, 
      claudin-5 and zonula occludens-1 (ZO-1) was undetectable at the central 
      subdivisions of the sensory CVOs but some was expressed at the distal 
      subdivisions. Electron microscopic observation showed that capillaries were 
      surrounded with numerous layers of astrocyte processes and dendrites. The 
      expression of occludin and ZO-1 was also observed as puncta on GFAP-positive 
      astrocytes/tanycytes of the sensory CVOs. Our study thus demonstrates the 
      heterogeneity of vascular permeability and expression of tight-junction proteins 
      and indicates that the outer basement membrane and dense astrocyte/tanycyte 
      connection are possible alternative mechanisms for a diffusion barrier of 
      blood-derived molecules, instead of the BBB.
FAU - Morita, Shoko
AU  - Morita S
AD  - Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, 
      Sakyo-ku, Kyoto, 606-8585, Japan.
AD  - Department of Anatomy and Neuroscience, Faculty of Medicine, Nara Medical 
      University, Shijo-cho, Kashihara, Nara, 634-8521, Japan.
FAU - Furube, Eriko
AU  - Furube E
AD  - Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, 
      Sakyo-ku, Kyoto, 606-8585, Japan.
FAU - Mannari, Tetsuya
AU  - Mannari T
AD  - Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, 
      Sakyo-ku, Kyoto, 606-8585, Japan.
FAU - Okuda, Hiroaki
AU  - Okuda H
AD  - Department of Anatomy and Neuroscience, Faculty of Medicine, Nara Medical 
      University, Shijo-cho, Kashihara, Nara, 634-8521, Japan.
FAU - Tatsumi, Kouko
AU  - Tatsumi K
AD  - Department of Anatomy and Neuroscience, Faculty of Medicine, Nara Medical 
      University, Shijo-cho, Kashihara, Nara, 634-8521, Japan.
FAU - Wanaka, Akio
AU  - Wanaka A
AD  - Department of Anatomy and Neuroscience, Faculty of Medicine, Nara Medical 
      University, Shijo-cho, Kashihara, Nara, 634-8521, Japan.
FAU - Miyata, Seiji
AU  - Miyata S
AD  - Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, 
      Sakyo-ku, Kyoto, 606-8585, Japan. smiyata@kit.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150606
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Antigens, CD)
RN  - 0 (Cadherins)
RN  - 0 (Claudin-5)
RN  - 0 (Occludin)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 0 (cadherin 5)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Blood-Brain Barrier/*physiology
MH  - Cadherins/metabolism
MH  - Capillary Permeability/*physiology
MH  - Circumventricular Organs/anatomy & histology/*blood supply/ultrastructure
MH  - Claudin-5/metabolism
MH  - Diffusion
MH  - Fluorescein-5-isothiocyanate/metabolism
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Models, Biological
MH  - Occludin/metabolism
MH  - Zonula Occludens-1 Protein/metabolism
OTO - NOTNLM
OT  - Astrocyte
OT  - Blood-brain barrier
OT  - Mouse
OT  - Perivascular space
OT  - Tight junction
OT  - Vascular permeability
EDAT- 2015/06/07 06:00
MHDA- 2016/10/25 06:00
CRDT- 2015/06/07 06:00
PHST- 2014/12/21 00:00 [received]
PHST- 2015/04/23 00:00 [accepted]
PHST- 2015/06/07 06:00 [entrez]
PHST- 2015/06/07 06:00 [pubmed]
PHST- 2016/10/25 06:00 [medline]
AID - 10.1007/s00441-015-2207-7 [pii]
AID - 10.1007/s00441-015-2207-7 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2016 Feb;363(2):497-511. doi: 10.1007/s00441-015-2207-7. Epub 
      2015 Jun 6.