PMID- 26051938
OWN - NLM
STAT- MEDLINE
DCOM- 20160324
LR  - 20150618
IS  - 2211-1247 (Electronic)
VI  - 11
IP  - 10
DP  - 2015 Jun 16
TI  - Aurora Kinase B Regulates Telomerase Activity via a Centromeric RNA in Stem 
      Cells.
PG  - 1667-78
LID - S2211-1247(15)00527-6 [pii]
LID - 10.1016/j.celrep.2015.05.015 [doi]
AB  - Non-coding RNAs can modulate histone modifications that, at the same time, affect 
      transcript expression levels. Here, we dissect such a network in mouse embryonic 
      stem cells (ESCs). It regulates the activity of the reverse transcriptase 
      telomerase, which synthesizes telomeric repeats at the chromosome ends. We find 
      that histone H3 serine 10 phosphorylation set by Aurora kinase B (AURKB) in ESCs 
      during the S phase of the cell cycle at centromeric and (sub)telomeric loci 
      promotes the expression of non-coding minor satellite RNA (cenRNA). Inhibition of 
      AURKB induces silencing of cenRNA transcription and establishment of a repressive 
      chromatin state with histone H3 lysine 9 trimethylation and heterochromatin 
      protein 1 accumulation. This process results in a continuous shortening of 
      telomeres. We further show that AURKB interacts with both telomerase and cenRNA 
      and activates telomerase in trans. Thus, in mouse ESCs, telomere maintenance is 
      regulated via expression of cenRNA in a cell-cycle-dependent manner.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Mallm, Jan-Philipp
AU  - Mallm JP
AD  - Research Group Genome Organization and Function, Deutsches Krebsforschungszentrum 
      (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany; Bioquant Center, Im 
      Neuenheimer Feld 267, 69120 Heidelberg, Germany. Electronic address: 
      j.mallm@dkfz.de.
FAU - Rippe, Karsten
AU  - Rippe K
AD  - Research Group Genome Organization and Function, Deutsches Krebsforschungszentrum 
      (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany; Bioquant Center, Im 
      Neuenheimer Feld 267, 69120 Heidelberg, Germany. Electronic address: 
      karsten.rippe@dkfz.de.
LA  - eng
SI  - GEO/GSE67133
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150604
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.11.1 (Aurora Kinase B)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Animals
MH  - Aurora Kinase B/genetics/*metabolism
MH  - Centromere/*metabolism
MH  - Embryonic Stem Cells/*enzymology
MH  - Mice
MH  - RNA/*metabolism
MH  - Telomerase/*metabolism
EDAT- 2015/06/09 06:00
MHDA- 2016/03/25 06:00
CRDT- 2015/06/09 06:00
PHST- 2015/01/09 00:00 [received]
PHST- 2015/03/28 00:00 [revised]
PHST- 2015/05/11 00:00 [accepted]
PHST- 2015/06/09 06:00 [entrez]
PHST- 2015/06/09 06:00 [pubmed]
PHST- 2016/03/25 06:00 [medline]
AID - S2211-1247(15)00527-6 [pii]
AID - 10.1016/j.celrep.2015.05.015 [doi]
PST - ppublish
SO  - Cell Rep. 2015 Jun 16;11(10):1667-78. doi: 10.1016/j.celrep.2015.05.015. Epub 
      2015 Jun 4.