PMID- 26053860
OWN - NLM
STAT- MEDLINE
DCOM- 20160304
LR  - 20201217
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 6
DP  - 2015
TI  - A Distinct Perisynaptic Glial Cell Type Forms Tripartite Neuromuscular Synapses 
      in the Drosophila Adult.
PG  - e0129957
LID - 10.1371/journal.pone.0129957 [doi]
LID - e0129957
AB  - Previous studies of Drosophila flight muscle neuromuscular synapses have revealed 
      their tripartite architecture and established an attractive experimental model 
      for genetic analysis of glial function in synaptic transmission. Here we extend 
      these findings by defining a new Drosophila glial cell type, designated 
      peripheral perisynaptic glia (PPG), which resides in the periphery and interacts 
      specifically with fine motor axon branches forming neuromuscular synapses. 
      Identification and specific labeling of PPG was achieved through cell 
      type-specific RNAi-mediated knockdown (KD) of a glial marker, Glutamine 
      Synthetase 2 (GS2). In addition, comparison among different Drosophila 
      neuromuscular synapse models from adult and larval developmental stages indicated 
      the presence of tripartite synapses on several different muscle types in the 
      adult. In contrast, PPG appear to be absent from larval body wall neuromuscular 
      synapses, which do not exhibit a tripartite architecture but rather are imbedded 
      in the muscle plasma membrane. Evolutionary conservation of tripartite synapse 
      architecture and peripheral perisynaptic glia in vertebrates and Drosophila 
      suggests ancient and conserved roles for glia-synapse interactions in synaptic 
      transmission.
FAU - Strauss, Alexandra L
AU  - Strauss AL
AD  - Department of Biology and Center for Molecular and Cellular Neuroscience, The 
      Pennsylvania State University, University Park, Pennsylvania, United States of 
      America.
FAU - Kawasaki, Fumiko
AU  - Kawasaki F
AD  - Department of Biology and Center for Molecular and Cellular Neuroscience, The 
      Pennsylvania State University, University Park, Pennsylvania, United States of 
      America.
FAU - Ordway, Richard W
AU  - Ordway RW
AD  - Department of Biology and Center for Molecular and Cellular Neuroscience, The 
      Pennsylvania State University, University Park, Pennsylvania, United States of 
      America.
LA  - eng
GR  - R01 NS065983/NS/NINDS NIH HHS/United States
GR  - 1R01NS065983-01A1/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150608
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Drosophila Proteins)
RN  - 0 (GAL4 protein, Drosophila)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/metabolism
MH  - Drosophila/*physiology
MH  - Drosophila Proteins/metabolism
MH  - Gene Expression
MH  - Gene Knockdown Techniques
MH  - Neuroglia/*metabolism
MH  - Neuromuscular Junction/*metabolism
MH  - Synaptic Transmission
MH  - Transcription Factors/metabolism
PMC - PMC4459971
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/06/09 06:00
MHDA- 2016/03/05 06:00
PMCR- 2015/06/08
CRDT- 2015/06/09 06:00
PHST- 2014/11/10 00:00 [received]
PHST- 2015/05/14 00:00 [accepted]
PHST- 2015/06/09 06:00 [entrez]
PHST- 2015/06/09 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PHST- 2015/06/08 00:00 [pmc-release]
AID - PONE-D-14-50658 [pii]
AID - 10.1371/journal.pone.0129957 [doi]
PST - epublish
SO  - PLoS One. 2015 Jun 8;10(6):e0129957. doi: 10.1371/journal.pone.0129957. 
      eCollection 2015.