PMID- 26055549
OWN - NLM
STAT- MEDLINE
DCOM- 20170404
LR  - 20170404
IS  - 0394-6320 (Print)
IS  - 0394-6320 (Linking)
VI  - 28
IP  - 2
DP  - 2015 Jun
TI  - Knockdown of CUL4A inhibits invasion and induces apoptosis in osteosarcoma cells.
PG  - 263-9
LID - 10.1177/0394632015586656 [doi]
AB  - Cullin4A (CUL4A) is implicated in many cellular events including cell survival 
      and growth. However, the specific function and underlying mechanisms of CUL4A in 
      cancer invasion have not yet been elucidated. In this work, we were focused on 
      investigating the role of CUL4A in human osteosarcoma (OS). The expression level 
      of CUL4A was evaluated by immunohistochemical (IHC) assay in human OS tissues. 
      Lentivirus-mediated CUL4A shRNA (Lv-shCUL4A) constructed by us was transfected 
      into OS cells for assessing its effects on cell proliferation and invasive 
      potential, respectively detected by MTT and Transwell assays. It was demonstrated 
      that the expression of CUL4A protein was markedly increased in OS tissues 
      compared with the adjacent non-cancerous tissues (ANCT) (57.8% vs. 25.6%, P = 
      0.019), and was associated with the distant metastases in OS patients (P = 
      0.016). In vitro, silencing of CUL4A gene inhibited OS cell proliferation and 
      invasion, and induced cell apoptosis, followed by increased expression of p27 and 
      p53 and decreased expression of MMP-2. Therefore, these findings indicate that 
      elevated expression of CUL4A is positively correlated with distant metastases in 
      OS patients, and knockdown of CUL4A suppresses invasion and induces apoptosis in 
      OS cells, suggesting that CUL4A may serve as a potential target for the treatment 
      of OS.
CI  - (c) The Author(s) 2015.
FAU - Song, Jia
AU  - Song J
AD  - Department of Orthopedic Surgery, Xinhua Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai, PR China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Orthopedic Surgery, Xinhua Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai, PR China.
FAU - Shao, Jiang
AU  - Shao J
AD  - Department of Orthopedic Surgery, Xinhua Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai, PR China shaojiang201502@163.com.
LA  - eng
PT  - Letter
DEP - 20150608
PL  - England
TA  - Int J Immunopathol Pharmacol
JT  - International journal of immunopathology and pharmacology
JID - 8911335
RN  - 0 (CUL4A protein, human)
RN  - 0 (Cullin Proteins)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Apoptosis/*genetics
MH  - Bone Neoplasms/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Cullin Proteins/*genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Gene Knockdown Techniques/methods
MH  - Humans
MH  - Neoplasm Invasiveness/*genetics/pathology
MH  - Osteosarcoma/*genetics/pathology
MH  - RNA, Small Interfering/genetics
OTO - NOTNLM
OT  - CUL4A
OT  - invasion
OT  - osteosarcoma
OT  - proliferation
EDAT- 2015/06/10 06:00
MHDA- 2017/04/05 06:00
CRDT- 2015/06/10 06:00
PHST- 2015/02/24 00:00 [received]
PHST- 2015/04/20 00:00 [accepted]
PHST- 2015/06/10 06:00 [entrez]
PHST- 2015/06/10 06:00 [pubmed]
PHST- 2017/04/05 06:00 [medline]
AID - 0394632015586656 [pii]
AID - 10.1177/0394632015586656 [doi]
PST - ppublish
SO  - Int J Immunopathol Pharmacol. 2015 Jun;28(2):263-9. doi: 
      10.1177/0394632015586656. Epub 2015 Jun 8.