PMID- 26055946
OWN - NLM
STAT- MEDLINE
DCOM- 20160527
LR  - 20161125
IS  - 1873-1708 (Electronic)
IS  - 0890-6238 (Linking)
VI  - 57
DP  - 2015 Nov
TI  - Steroidogenic differential effects in neonatal porcine Leydig cells exposed to 
      persistent organic pollutants derived from cod liver oil.
PG  - 130-9
LID - S0890-6238(15)00107-0 [pii]
LID - 10.1016/j.reprotox.2015.05.016 [doi]
AB  - Seafood products, including fish and fish oils, are major sources of persistent 
      organic pollutants (POPs) which may cause endocrine disruption related to 
      reproductive dysfunction in males. Primary porcine neonatal Leydig cells were 
      exposed to three extracts of POPs obtained from different stages in production of 
      cod liver oil dietary supplement, in the absence and presence of luteinizing 
      hormone (LH). No reduced viability was observed and all POP extracts showed 
      increased testosterone and estradiol levels in unstimulated cells and decreased 
      testosterone and estradiol secretion in LH-stimulated cells. A decrease in 
      central steriodogenic genes including STAR, CYP11A1, HSD3B and CYP17A1 was 
      obtained in both culture conditions with all POP extracts. We implicate both 
      small differences in composition and concentration of compounds as well as "old" 
      POPs to be important for the observed steroidogenic effects.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Granum, Cesilie
AU  - Granum C
AD  - Department of Production Animal Clinical Sciences, Norwegian University of Live 
      Sciences, NMBU-School of Veterinary Science, P.O. 8146 Dep, 0033 Oslo, Norway. 
      Electronic address: cesilie.granum@gmail.com.
FAU - Anchersen, Sara
AU  - Anchersen S
AD  - Department of Production Animal Clinical Sciences, Norwegian University of Live 
      Sciences, NMBU-School of Veterinary Science, P.O. 8146 Dep, 0033 Oslo, Norway. 
      Electronic address: sara.anchersen@fhi.no.
FAU - Karlsson, Camilla
AU  - Karlsson C
AD  - Department of Production Animal Clinical Sciences, Norwegian University of Live 
      Sciences, NMBU-School of Veterinary Science, P.O. 8146 Dep, 0033 Oslo, Norway. 
      Electronic address: camillakarlsson10@gmail.com.
FAU - Berg, Vidar
AU  - Berg V
AD  - Department of Production Animal Clinical Sciences, Norwegian University of Live 
      Sciences, NMBU-School of Veterinary Science, P.O. 8146 Dep, 0033 Oslo, Norway. 
      Electronic address: vidar.berg@nmbu.no.
FAU - Olsaker, Ingrid
AU  - Olsaker I
AD  - Department of Production Animal Clinical Sciences, Norwegian University of Live 
      Sciences, NMBU-School of Veterinary Science, P.O. 8146 Dep, 0033 Oslo, Norway; 
      Department of Basic Sciences and Aquatic Medicine, Norwegian University of Live 
      Sciences, NMBU-School of Veterinary Science, P.O. 8146 Dep, 0033 Oslo, Norway. 
      Electronic address: ingrid.olsaker@nmbu.no.
FAU - Verhaegen, Steven
AU  - Verhaegen S
AD  - Department of Production Animal Clinical Sciences, Norwegian University of Live 
      Sciences, NMBU-School of Veterinary Science, P.O. 8146 Dep, 0033 Oslo, Norway. 
      Electronic address: steven.verhaegen@nmbu.no.
FAU - Ropstad, Erik
AU  - Ropstad E
AD  - Department of Production Animal Clinical Sciences, Norwegian University of Live 
      Sciences, NMBU-School of Veterinary Science, P.O. 8146 Dep, 0033 Oslo, Norway. 
      Electronic address: erik.ropstad@nmbu.no.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150606
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Phosphoproteins)
RN  - 3XMK78S47O (Testosterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 8001-69-2 (Cod Liver Oil)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 1.1.1.145 (Progesterone Reductase)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
RN  - EC 5.3.3.- (Steroid Isomerases)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cells, Cultured
MH  - Cholesterol Side-Chain Cleavage Enzyme/genetics
MH  - Cod Liver Oil/*chemistry
MH  - Endocrine Disruptors/*toxicity
MH  - Environmental Pollutants/*toxicity
MH  - Estradiol/metabolism
MH  - Gene Expression/drug effects
MH  - Hydrocarbons, Chlorinated/*toxicity
MH  - Leydig Cells/*drug effects/metabolism
MH  - Luteinizing Hormone/pharmacology
MH  - Male
MH  - Multienzyme Complexes/genetics
MH  - Phosphoproteins/genetics
MH  - Progesterone Reductase/genetics
MH  - Steroid 17-alpha-Hydroxylase/genetics
MH  - Steroid Isomerases/genetics
MH  - Swine
MH  - Testosterone/metabolism
EDAT- 2015/06/10 06:00
MHDA- 2016/05/28 06:00
CRDT- 2015/06/10 06:00
PHST- 2013/01/23 00:00 [received]
PHST- 2015/05/20 00:00 [revised]
PHST- 2015/05/28 00:00 [accepted]
PHST- 2015/06/10 06:00 [entrez]
PHST- 2015/06/10 06:00 [pubmed]
PHST- 2016/05/28 06:00 [medline]
AID - S0890-6238(15)00107-0 [pii]
AID - 10.1016/j.reprotox.2015.05.016 [doi]
PST - ppublish
SO  - Reprod Toxicol. 2015 Nov;57:130-9. doi: 10.1016/j.reprotox.2015.05.016. Epub 2015 
      Jun 6.