PMID- 26056387
OWN - NLM
STAT- MEDLINE
DCOM- 20150924
LR  - 20181113
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 83
IP  - 8
DP  - 2015 Aug
TI  - The Staphylococcus aureus protein-coding gene gdpS modulates sarS expression via 
      mRNA-mRNA interaction.
PG  - 3302-10
LID - 10.1128/IAI.00159-15 [doi]
AB  - Staphylococcus aureus is an important Gram-positive pathogen responsible for 
      numerous diseases ranging from localized skin infections to life-threatening 
      systemic infections. The virulence of S. aureus is essentially determined by a 
      wide spectrum of factors, including cell wall-associated proteins and secreted 
      toxins that are precisely controlled in response to environmental changes. GGDEF 
      domain protein from Staphylococcus (GdpS) is the only conserved staphylococcal 
      GGDEF domain protein that is involved not in c-di-GMP synthesis but in the 
      virulence regulation of S. aureus NCTC8325. Our previous study showed that the 
      inactivation of gdpS generates an extensive change of virulence factors together 
      with, in particular, a major Spa (protein A) surface protein. As reported, sarS 
      is a direct positive regulator of spa. The decreased transcript levels of sarS in 
      the gdpS mutant compared with the parental NCTC8325 strain suggest that gdpS 
      affects spa through interaction with sarS. In this study, site mutation and 
      complementary experiments showed that the translation product of gdpS was not 
      involved in the regulation of transcript levels of sarS. We found that gdpS 
      functioned through direct RNA-RNA base pairing with the 5' untranslated region 
      (5'UTR) of sarS mRNA and that a putative 18-nucleotide region played a 
      significant role in the regulatory process. Furthermore, the mRNA half-life 
      analysis of sarS in the gdpS mutant showed that gdpS positively regulates the 
      mRNA levels of sarS by contributing to the stabilization of sarS mRNA, suggesting 
      that gdpS mRNA may regulate spa expression in an RNA-dependent pathway.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Chen, Chuan
AU  - Chen C
AD  - School of Life Sciences and CAS Key Laboratory of Innate Immunity and Chronic 
      Disease, University of Science and Technology of China, Hefei, Anhui, China.
FAU - Zhang, Xu
AU  - Zhang X
AD  - School of Life Sciences and CAS Key Laboratory of Innate Immunity and Chronic 
      Disease, University of Science and Technology of China, Hefei, Anhui, China.
FAU - Shang, Fei
AU  - Shang F
AD  - School of Life Sciences, Anhui Agricultural University, Hefei, Anhui, China.
FAU - Sun, Haipeng
AU  - Sun H
AD  - School of Life Sciences and CAS Key Laboratory of Innate Immunity and Chronic 
      Disease, University of Science and Technology of China, Hefei, Anhui, China.
FAU - Sun, Baolin
AU  - Sun B
AD  - School of Life Sciences and CAS Key Laboratory of Innate Immunity and Chronic 
      Disease, University of Science and Technology of China, Hefei, Anhui, China 
      sunb@ustc.edu.cn xuet@ahau.edu.cn.
FAU - Xue, Ting
AU  - Xue T
AD  - School of Life Sciences and CAS Key Laboratory of Innate Immunity and Chronic 
      Disease, University of Science and Technology of China, Hefei, Anhui, China 
      School of Life Sciences, Anhui Agricultural University, Hefei, Anhui, China 
      sunb@ustc.edu.cn xuet@ahau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150608
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Messenger)
RN  - 0 (SarS protein, Staphylococcus aureus)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Bacterial Proteins/*genetics/metabolism
MH  - Base Sequence
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - *Gene Expression Regulation, Bacterial
MH  - Molecular Sequence Data
MH  - RNA, Bacterial/genetics/metabolism
MH  - RNA, Messenger/genetics/*metabolism
MH  - Staphylococcal Infections/*microbiology
MH  - Staphylococcus aureus/genetics/*metabolism
PMC - PMC4496595
EDAT- 2015/06/10 06:00
MHDA- 2015/09/25 06:00
PMCR- 2016/02/01
CRDT- 2015/06/10 06:00
PHST- 2015/02/09 00:00 [received]
PHST- 2015/05/29 00:00 [accepted]
PHST- 2015/06/10 06:00 [entrez]
PHST- 2015/06/10 06:00 [pubmed]
PHST- 2015/09/25 06:00 [medline]
PHST- 2016/02/01 00:00 [pmc-release]
AID - IAI.00159-15 [pii]
AID - 00159-15 [pii]
AID - 10.1128/IAI.00159-15 [doi]
PST - ppublish
SO  - Infect Immun. 2015 Aug;83(8):3302-10. doi: 10.1128/IAI.00159-15. Epub 2015 Jun 8.