PMID- 26056776 OWN - NLM STAT- MEDLINE DCOM- 20160420 LR - 20150710 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) VI - 192 DP - 2015 Sep TI - Advanced nitrogen removal via nitrite using stored polymers in a modified sequencing batch reactor treating landfill leachate. PG - 354-60 LID - S0960-8524(15)00677-X [pii] LID - 10.1016/j.biortech.2015.05.013 [doi] AB - A modified sequencing batch reactor (SBR) operated at the anaerobic-aerobic-anoxic mode was developed in this study to fully utilize the organics in landfill leachate (ammonia concentration of 1000 +/- 50 mg N/L and COD/total nitrogen (TN) ratio of 1-4). The unique feature of modified SBR process was the addition of an anaerobic stage after feeding stage, so that microorganisms could store the organics during anaerobic stage and supply the carbon source for endogenous denitritation after aeration stage. The 70-day operational tests showed the effluent TN was below 10 mg N/L at C/N ratio of 4. The intracellular stored polymers were analyzed and the microorganisms were capable of storing the carbon source as polyhydroxybutyrate (PHB) and glycogen in anaerobic stage, which were the electron donors for endogenous denitritation. Fluorescence in situ hybridization (FISH) analysis showed that glycogen accumulating organisms (GAOs) account for 39.8% of microorganisms in SBR, and carried out advanced nitrogen removal. CI - Copyright (c) 2015 Elsevier Ltd. All rights reserved. FAU - Miao, Lei AU - Miao L AD - Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China. Electronic address: miaolei2819@emails.bjut.edu.cn. FAU - Wang, Shuying AU - Wang S AD - Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China. FAU - Li, Baikun AU - Li B AD - Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT, USA. FAU - Cao, Tianhao AU - Cao T AD - Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China. FAU - Xue, Tonglai AU - Xue T AD - Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China. FAU - Peng, Yongzhen AU - Peng Y AD - Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China. Electronic address: pyz@bjut.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150514 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 0 (Nitrites) RN - 0 (Polyesters) RN - 0 (Water Pollutants, Chemical) RN - 7440-44-0 (Carbon) RN - 9005-79-2 (Glycogen) RN - N762921K75 (Nitrogen) SB - IM MH - Anaerobiosis MH - Biological Oxygen Demand Analysis MH - Bioreactors/*microbiology MH - Carbon/metabolism MH - Equipment Design MH - Glycogen/*metabolism MH - In Situ Hybridization, Fluorescence MH - Microbial Consortia/genetics/*physiology MH - Nitrites/*metabolism MH - Nitrogen/*isolation & purification/metabolism MH - Polyesters/*metabolism MH - Waste Disposal, Fluid/instrumentation/*methods MH - Water Pollutants, Chemical/chemistry/*metabolism OTO - NOTNLM OT - FISH OT - GAOs OT - Landfill leachate OT - Nitrogen removal OT - SBR EDAT- 2015/06/10 06:00 MHDA- 2016/04/21 06:00 CRDT- 2015/06/10 06:00 PHST- 2015/04/03 00:00 [received] PHST- 2015/05/05 00:00 [revised] PHST- 2015/05/07 00:00 [accepted] PHST- 2015/06/10 06:00 [entrez] PHST- 2015/06/10 06:00 [pubmed] PHST- 2016/04/21 06:00 [medline] AID - S0960-8524(15)00677-X [pii] AID - 10.1016/j.biortech.2015.05.013 [doi] PST - ppublish SO - Bioresour Technol. 2015 Sep;192:354-60. doi: 10.1016/j.biortech.2015.05.013. Epub 2015 May 14.