PMID- 26057701
OWN - NLM
STAT- MEDLINE
DCOM- 20160626
LR  - 20181113
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Print)
IS  - 1936-0851 (Linking)
VI  - 9
IP  - 7
DP  - 2015 Jul 28
TI  - Engineered Nanostructures of Haptens Lead to Unexpected Formation of Membrane 
      Nanotubes Connecting Rat Basophilic Leukemia Cells.
PG  - 6738-46
LID - 10.1021/acsnano.5b02270 [doi]
AB  - A recent finding reports that co-stimulation of the high-affinity immunoglobulin 
      E (IgE) receptor (FcepsilonRI) and the chemokine receptor 1 (CCR1) triggered formation 
      of membrane nanotubes among bone-marrow-derived mast cells. The co-stimulation 
      was attained using corresponding ligands: IgE binding antigen and macrophage 
      inflammatory protein 1alpha (MIP1 alpha), respectively. However, this approach failed to 
      trigger formation of nanotubes among rat basophilic leukemia (RBL) cells due to 
      the lack of CCR1 on the cell surface (Int. Immunol. 2010, 22 (2), 113-128). RBL 
      cells are frequently used as a model for mast cells and are best known for 
      antibody-mediated activation via FcepsilonRI. This work reports the successful 
      formation of membrane nanotubes among RBLs using only one stimulus, a hapten of 
      2,4-dinitrophenyl (DNP) molecules, which are presented as nanostructures with our 
      designed spatial arrangements. This observation underlines the significance of 
      the local presentation of ligands in the context of impacting the cellular 
      signaling cascades. In the case of RBL, certain DNP nanostructures suppress 
      antigen-induced degranulation and facilitate the rearrangement of the 
      cytoskeleton to form nanotubes. These results demonstrate an important scientific 
      concept; engineered nanostructures enable cellular signaling cascades, where 
      current technologies encounter great difficulties. More importantly, 
      nanotechnology offers a new platform to selectively activate and/or inhibit 
      desired cellular signaling cascades.
FAU - Li, Jie-Ren
AU  - Li JR
AD  - daggerDepartment of Chemistry, University of California, Davis, California 95616, 
      United States.
FAU - Ross, Shailise S
AU  - Ross SS
AD  - daggerDepartment of Chemistry, University of California, Davis, California 95616, 
      United States.
FAU - Liu, Yang
AU  - Liu Y
AD  - daggerDepartment of Chemistry, University of California, Davis, California 95616, 
      United States.
FAU - Liu, Ying X
AU  - Liu YX
AD  - daggerDepartment of Chemistry, University of California, Davis, California 95616, 
      United States.
FAU - Wang, Kang-Hsin
AU  - Wang KH
AD  - daggerDepartment of Chemistry, University of California, Davis, California 95616, 
      United States.
FAU - Chen, Huan-Yuan
AU  - Chen HY
AD  - double daggerDepartment of Dermatology, School of Medicine, University of California, Davis, 
      Sacramento, California 95817, United States.
AD  -  section signInstitute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.
FAU - Liu, Fu-Tong
AU  - Liu FT
AD  - double daggerDepartment of Dermatology, School of Medicine, University of California, Davis, 
      Sacramento, California 95817, United States.
AD  -  section signInstitute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.
FAU - Laurence, Ted A
AU  - Laurence TA
AD  -  parallelLawrence Livermore National Laboratory, Livermore, California 94550, United 
      States.
FAU - Liu, Gang-Yu
AU  - Liu GY
AD  - daggerDepartment of Chemistry, University of California, Davis, California 95616, 
      United States.
LA  - eng
GR  - R01 AR056343/AR/NIAMS NIH HHS/United States
GR  - R21 CA176850/CA/NCI NIH HHS/United States
GR  - 1R21CA176850-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150618
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (Haptens)
SB  - IM
MH  - Animals
MH  - Basophils/*ultrastructure
MH  - Cell Line, Tumor
MH  - Cell Membrane Structures/drug effects/*ultrastructure
MH  - Haptens/*chemistry/pharmacology
MH  - Nanostructures/*chemistry
MH  - Rats
PMC - PMC4758354
MID - NIHMS758956
OTO - NOTNLM
OT  - atomic force microscopy (AFM)
OT  - haptens
OT  - mast cells
OT  - membrane nanotubes
OT  - particle lithography
OT  - rat basophilic leukemia (RBL) cells
OT  - scanning electron microscopy (SEM)
COIS- Conflict of Interest: The authors declare no competing financial interest.
EDAT- 2015/06/10 06:00
MHDA- 2016/06/28 06:00
PMCR- 2016/02/18
CRDT- 2015/06/10 06:00
PHST- 2015/06/10 06:00 [entrez]
PHST- 2015/06/10 06:00 [pubmed]
PHST- 2016/06/28 06:00 [medline]
PHST- 2016/02/18 00:00 [pmc-release]
AID - 10.1021/acsnano.5b02270 [doi]
PST - ppublish
SO  - ACS Nano. 2015 Jul 28;9(7):6738-46. doi: 10.1021/acsnano.5b02270. Epub 2015 Jun 
      18.