PMID- 26061570
OWN - NLM
STAT- MEDLINE
DCOM- 20160713
LR  - 20220309
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Linking)
VI  - 18
IP  - 10
DP  - 2015 Oct
TI  - Antioxidant Opuntia ficus-indica Extract Activates AHR-NRF2 Signaling and 
      Upregulates Filaggrin and Loricrin Expression in Human Keratinocytes.
PG  - 1143-9
LID - 10.1089/jmf.2014.3396 [doi]
AB  - Opuntia ficus-indica (OFI) is a cactus species widely used as an 
      anti-inflammatory, antilipidemic, and hypoglycemic agent. It has been shown that 
      OFI extract (OFIE) inhibits oxidative stress in animal models of diabetes and 
      hepatic disease; however, its antioxidant mechanism remains largely unknown. In 
      this study, we demonstrated that OFIE exhibited potent antioxidant activity 
      through the activation of nuclear factor erythroid 2-related factor 2 (NRF2) and 
      the downstream antioxidant enzyme NAD(P)H: quinone oxidoreductase 1 (NQO1), which 
      inhibited the generation of reactive oxygen species in keratinocytes challenged 
      with tumor necrosis factor alpha or benzo[alpha]pyrene. The antioxidant capacity of OFIE 
      was canceled in NRF2 knockdown keratinocytes. OFIE exerted this NRF2-NQO1 
      upregulation through activation of the aryl hydrocarbon receptor (AHR). Moreover, 
      the ligation of AHR by OFIE upregulated the expression of epidermal barrier 
      proteins: filaggrin and loricrin. OFIE also prevented TH2 cytokine-mediated 
      downregulation of filaggrin and loricrin expression in an AHR-dependent manner 
      because it was canceled in AHR knockdown keratinocytes. Antioxidant OFIE is a 
      potent activator of AHR-NRF2-NQO1 signaling and may be beneficial in treating 
      barrier-disrupted skin disorders.
FAU - Nakahara, Takeshi
AU  - Nakahara T
AD  - 1 Division of Skin Surface Sensing, Kyushu University , Fukuoka, Japan .
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
FAU - Mitoma, Chikage
AU  - Mitoma C
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
AD  - 3 Research and Clinical Center for Yusho and Dioxin, Kyushu University , Fukuoka, 
      Japan .
FAU - Hashimoto-Hachiya, Akiko
AU  - Hashimoto-Hachiya A
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
FAU - Takahara, Masakazu
AU  - Takahara M
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
FAU - Tsuji, Gaku
AU  - Tsuji G
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
FAU - Uchi, Hiroshi
AU  - Uchi H
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
AD  - 3 Research and Clinical Center for Yusho and Dioxin, Kyushu University , Fukuoka, 
      Japan .
FAU - Yan, Xianghong
AU  - Yan X
AD  - 4 P&G Innovation Godo Kaisha , Kobe, Japan .
FAU - Hachisuka, Junichi
AU  - Hachisuka J
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
FAU - Chiba, Takahito
AU  - Chiba T
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
FAU - Esaki, Hitokazu
AU  - Esaki H
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
FAU - Kido-Nakahara, Makiko
AU  - Kido-Nakahara M
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
FAU - Furue, Masutaka
AU  - Furue M
AD  - 1 Division of Skin Surface Sensing, Kyushu University , Fukuoka, Japan .
AD  - 2 Department of Dermatology, Kyushu University , Fukuoka, Japan .
AD  - 3 Research and Clinical Center for Yusho and Dioxin, Kyushu University , Fukuoka, 
      Japan .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150518
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Antioxidants)
RN  - 0 (FLG protein, human)
RN  - 0 (Filaggrin Proteins)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Plant Extracts)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (loricrin)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (NQO1 protein, human)
SB  - IM
MH  - Antioxidants/pharmacology
MH  - Cells, Cultured
MH  - Filaggrin Proteins
MH  - Gene Expression/drug effects
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Intermediate Filament Proteins/*analysis
MH  - Keratinocytes/chemistry/*drug effects/metabolism
MH  - Membrane Proteins/*analysis
MH  - NAD(P)H Dehydrogenase (Quinone)/metabolism
MH  - NF-E2-Related Factor 2/deficiency/genetics/*physiology
MH  - Opuntia/*chemistry
MH  - Plant Extracts/pharmacology
MH  - Receptors, Aryl Hydrocarbon/drug effects/*metabolism
MH  - Signal Transduction/drug effects
MH  - Skin Diseases/drug therapy
OTO - NOTNLM
OT  - NQO1
OT  - NRF2
OT  - Opuntia ficus-indica extract
OT  - antioxidant
OT  - aryl hydrocarbon receptor
OT  - filaggrin
EDAT- 2015/06/11 06:00
MHDA- 2016/07/14 06:00
CRDT- 2015/06/11 06:00
PHST- 2015/06/11 06:00 [entrez]
PHST- 2015/06/11 06:00 [pubmed]
PHST- 2016/07/14 06:00 [medline]
AID - 10.1089/jmf.2014.3396 [doi]
PST - ppublish
SO  - J Med Food. 2015 Oct;18(10):1143-9. doi: 10.1089/jmf.2014.3396. Epub 2015 May 18.