PMID- 26062411
OWN - NLM
STAT- MEDLINE
DCOM- 20151112
LR  - 20150611
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 31
IP  - 6
DP  - 2015 Jun
TI  - [Pneumococcal HSP40 induces the immune response in mouse macrophages via p38MAPK 
      and JNK signaling pathways].
PG  - 730-5
AB  - OBJECTIVE: To investigate the mechanism of immune response in mouse macrophage 
      induced by Pneumococcal heat shock protein 40 (HSP40). METHODS: After recombinant 
      HSP40 (rHSP40) underwent expression detection and purification, 
      lipopolysaccharide (LPS) was removed from it. Then rHSP40 was used to stimulate 
      bone marrow derived macrophages (BMDMs) derived from C57BL/6 wild-type mice. The 
      mRNA levels of tumor necrosis factor alpha (TNF-alpha), interleukin 6 (IL-6), IL-1beta, 
      IL-23p19, IL-12p40, IL-12p35 and IL-10 in BMDMs were determined by reverse 
      transcription PCR; the expressions of TNF-alpha, IL-6 and IL-12p40 were measured by 
      ELISA. After stimulated by rHSP40, the levels of TNF-alpha and IL-6 expressed by 
      wide-type, TLR2-/- and TLR4-/- BMDMs were detected by ELISA. The effects of the 
      pretreatment of mitogen-activated protein kinases (MAPK) inhibitors on the 
      secretion of TNF-alpha and IL-6 induced by rHSP40 were also evaluated by ELISA in 
      BMDMs. The phosphorylation levels of p38MAPK and c-Jun N-terminal kinase (JNK) 
      were determined by Western blotting. RESULTS: The rHSP40 protein reached a purity 
      of more than 90%. It significantly enhanced the phosphorylation levels of p38MAPK 
      and JNK as well as the expressions of TNF-alpha and IL-6. The p38MAPK and JNK 
      inhibitors significantly suppressed the expressions of TNF-alpha and IL-6. The 
      expressions of TNF-alpha and IL-6 in TLR4-/- BMDMs significantly decreased compared 
      with wide-type BMDMs. CONCLUSION: HSP40-induced immune response of mouse 
      macrophages is regulated by p38MAPK and JNK signaling pathways, and this 
      induction process depends on TLR4.
FAU - Wu, Yingying
AU  - Wu Y
AD  - Ministry of Education Key Laboratory of Medical Diagnostics, Department of 
      Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Gao, Song
AU  - Gao S
AD  - Ministry of Education Key Laboratory of Medical Diagnostics, Department of 
      Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Ma, Feng
AU  - Ma F
AD  - Ministry of Education Key Laboratory of Medical Diagnostics, Department of 
      Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Cui, Jingjing
AU  - Cui J
AD  - Ministry of Education Key Laboratory of Medical Diagnostics, Department of 
      Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Yao, Hua
AU  - Yao H
AD  - Ministry of Education Key Laboratory of Medical Diagnostics, Department of 
      Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Sun, Xiaoyu
AU  - Sun X
AD  - Ministry of Education Key Laboratory of Medical Diagnostics, Department of 
      Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Wang, Jichao
AU  - Wang J
AD  - Ministry of Education Key Laboratory of Medical Diagnostics, Department of 
      Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
FAU - Xu, Wenchun
AU  - Xu W
AD  - Ministry of Education Key Laboratory of Medical Diagnostics, Department of 
      Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cytokines)
RN  - 0 (HSP40 Heat-Shock Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/immunology
MH  - Bone Marrow Cells/cytology
MH  - Cytokines/genetics
MH  - Gene Expression Regulation/immunology
MH  - HSP40 Heat-Shock Proteins/chemistry/*immunology
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - Lipopolysaccharides/isolation & purification
MH  - MAP Kinase Signaling System/*immunology
MH  - Macrophages/*cytology/*immunology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phosphorylation/immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Streptococcus pneumoniae/*immunology
MH  - Toll-Like Receptor 2/metabolism
MH  - Toll-Like Receptor 4/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2015/06/13 06:00
MHDA- 2015/11/13 06:00
CRDT- 2015/06/12 06:00
PHST- 2015/06/12 06:00 [entrez]
PHST- 2015/06/13 06:00 [pubmed]
PHST- 2015/11/13 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Jun;31(6):730-5.