PMID- 26062549
OWN - NLM
STAT- MEDLINE
DCOM- 20160314
LR  - 20181113
IS  - 1475-925X (Electronic)
IS  - 1475-925X (Linking)
VI  - 14
DP  - 2015 Jun 11
TI  - Dynamic expressions of monocyte chemo attractant protein-1 and CC chamomile 
      receptor 2 after balloon injury and their effects in intimal proliferation.
PG  - 55
LID - 10.1186/s12938-015-0030-8 [doi]
LID - 55
AB  - OBJECTIVE: The dynamic expressions of monocyte chemo attractant protein-1 (MCP-1) 
      and CC chamomile receptor 2 (CCR2) after balloon injury and their effects in 
      intimal proliferation were discussed. In this study, the expression of MCP-1 and 
      its receptor during the intimal proliferation in rat artery after balloon injury 
      were studied. METHODS: Using the model of balloon injury of rats' arteries, the 
      changes of intimal proliferation were observed with optical microscopy and the 
      expressions of MCP-1 and CCR2 at different times were examined with the methods 
      of RT-PCR and immunohistochemistry. The expressions of MCP-1 and CCR2 in the 
      arterial tissues were detected using reverse transcription polymerase chain 
      reaction (RT-PCR) and analyzed by semi-quantitative method. RESULTS: The 
      expressions of MCP-1 and CCR2 mRNA began to gradually increase after balloon 
      injury. The MCP-1 reached to the peak on the first day, but decreased gradually 
      later on. Expressions of CCR2 mRNA began to increase on the first day and reached 
      to the peak on the 7th day, but then started to decrease gradually until 28th day 
      when we can still detect it. The expressions of MCP-1 proteins began to increase 
      gradually after balloon injury and were obviously detected in the VSMC on the 4th 
      and 7th day, until 14th day when we can still detect it clearly in the 
      proliferating intima. CONCLUSION: The dynamic expressions of MCP-1, MCP-1 
      proteins and CCR2 mRNA after balloon injury were shown to play an important role 
      in intimal proliferation.
FAU - Huang, Zhigang
AU  - Huang Z
AD  - Emergency Department, Peking University Shenzhen Hospital, Shenzhen, 518036, 
      China. dochzg@163.com.
FAU - Li, Yuebing
AU  - Li Y
AD  - The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 
      310005, China. liyuebing1853@163.com.
FAU - Niu, Lili
AU  - Niu L
AD  - Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical 
      and Health Engineering, Shenzhen Institutes of Advanced Technology, Shenzhen, 
      518055, China. ll.niu@siat.ac.cn.
FAU - Xiao, Yang
AU  - Xiao Y
AD  - Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical 
      and Health Engineering, Shenzhen Institutes of Advanced Technology, Shenzhen, 
      518055, China. Yang.xiao@siat.ac.cn.
FAU - Pu, Xiaodong
AU  - Pu X
AD  - The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 
      310005, China. puxdmd@163.com.
FAU - Zheng, Hairong
AU  - Zheng H
AD  - Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical 
      and Health Engineering, Shenzhen Institutes of Advanced Technology, Shenzhen, 
      518055, China. hr.zheng@siat.ac.cn.
FAU - Qian, Ming
AU  - Qian M
AD  - Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical 
      and Health Engineering, Shenzhen Institutes of Advanced Technology, Shenzhen, 
      518055, China. ming.qian@siat.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150611
PL  - England
TA  - Biomed Eng Online
JT  - Biomedical engineering online
JID - 101147518
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Ccr2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
SB  - IM
MH  - Angioplasty, Balloon, Coronary/adverse effects
MH  - Animals
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - *Gene Expression Regulation
MH  - Hyperplasia
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Receptors, CCR2/*biosynthesis/genetics
MH  - Tunica Intima/*injuries/metabolism/pathology
MH  - Tunica Media/injuries/metabolism/pathology
PMC - PMC4469411
EDAT- 2015/06/13 06:00
MHDA- 2016/03/15 06:00
PMCR- 2015/06/11
CRDT- 2015/06/12 06:00
PHST- 2015/03/26 00:00 [received]
PHST- 2015/04/02 00:00 [accepted]
PHST- 2015/06/12 06:00 [entrez]
PHST- 2015/06/13 06:00 [pubmed]
PHST- 2016/03/15 06:00 [medline]
PHST- 2015/06/11 00:00 [pmc-release]
AID - 10.1186/s12938-015-0030-8 [pii]
AID - 30 [pii]
AID - 10.1186/s12938-015-0030-8 [doi]
PST - epublish
SO  - Biomed Eng Online. 2015 Jun 11;14:55. doi: 10.1186/s12938-015-0030-8.