PMID- 26062931 OWN - NLM STAT- MEDLINE DCOM- 20160321 LR - 20240117 IS - 1532-429X (Electronic) IS - 1097-6647 (Print) IS - 1097-6647 (Linking) VI - 17 IP - 1 DP - 2015 Jun 11 TI - Histological Validation of measurement of diffuse interstitial myocardial fibrosis by myocardial extravascular volume fraction from Modified Look-Locker imaging (MOLLI) T1 mapping at 3 T. PG - 48 LID - 10.1186/s12968-015-0150-0 [doi] LID - 48 AB - BACKGROUND: Gadolinium (Gd) Extracellular volume fraction (ECV) by Cardiovascular Magnetic Resonance (CMR) has been proposed as a non-invasive method for assessment of diffuse myocardial fibrosis. Yet only few studies used 3 T CMR to measure ECV, and the accuracy of ECV measurements at 3 T has not been established. Therefore the aims of the present study were to validate measurement of ECV by MOLLI T1 mapping by 3 T CMR against fibrosis measured by histopathology. We also evaluated the recently proposed hypothesis that native-T1 mapping without contrast injection would be sufficient to detect fibrosis. METHODS: 31 patients (age = 58 +/- 17 years, 77% men) with either severe aortic stenosis (n = 12) severe aortic regurgitation (n = 9) or severe mitral regurgitation (n = 10), all free of coronary artery disease, underwent 3 T-CMR with late gadolinium enhancement (LGE) and pre- and post-contrast MOLLI T1 mapping and ECV computation, prior to valve surgery. LV biopsies were performed at the time of surgery, a median 13 [1-30] days later, and stained with picrosirius red. Pre-, and post-contrast T1 values, ECV, and amount of LGE were compared against magnitude of fibrosis by histopathology by Pearson correlation coefficients. RESULTS: The average amount of interstitial fibrosis by picrosirius red staining in biopsy samples was 6.1 +/- 4.3%. ECV computed from pre-post contrast MOLLI T1 time changes was 28.9 +/- 5.5%, and correlated (r = 0.78, p < 0.001) strongly with the magnitude of histological fibrosis. By opposition, neither amount of LGE (r = 0.17, p = 0.36) nor native pre-contrast myocardial T1 time (r = -0.18, p = 0.32) correlated with fibrosis by histopathology. CONCLUSIONS: ECV determined by 3 T CMR T1 MOLLI images closely correlates with histologically determined diffuse interstitial fibrosis, providing a non-invasive estimation for quantification of interstitial fibrosis in patients with valve diseases. By opposition, neither non-contrast T1 times nor the amount of LGE were indicative of the magnitude of diffuse interstitial fibrosis measured by histopathology. FAU - de Meester de Ravenstein, Christophe AU - de Meester de Ravenstein C AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. christophe.demeester@uclouvain.be. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. christophe.demeester@uclouvain.be. FAU - Bouzin, Caroline AU - Bouzin C AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. caroline.bouzin@uclouvain.be. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. caroline.bouzin@uclouvain.be. FAU - Lazam, Siham AU - Lazam S AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. siham.lazam@uclouvain.be. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. siham.lazam@uclouvain.be. FAU - Boulif, Jamila AU - Boulif J AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. jamila.boulif@uclouvain.be. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. jamila.boulif@uclouvain.be. FAU - Amzulescu, Mihaela AU - Amzulescu M AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. amzulescum@yahoo.com. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. amzulescum@yahoo.com. FAU - Melchior, Julie AU - Melchior J AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. julie.melchior@gmail.com. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. julie.melchior@gmail.com. FAU - Pasquet, Agnes AU - Pasquet A AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. agnes.pasquet@uclouvain.be. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. agnes.pasquet@uclouvain.be. FAU - Vancraeynest, David AU - Vancraeynest D AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. david.vancraeynest@uclouvain.be. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. david.vancraeynest@uclouvain.be. FAU - Pouleur, Anne-Catherine AU - Pouleur AC AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. anne-catherine.pouleur@uclouvain.be. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. anne-catherine.pouleur@uclouvain.be. FAU - Vanoverschelde, Jean-Louis J AU - Vanoverschelde JL AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. jean-louis.vanoverschelde@uclouvain.be. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. jean-louis.vanoverschelde@uclouvain.be. FAU - Gerber, Bernhard L AU - Gerber BL AD - Division of Cardiology, Department of Cardiovascular Diseases, Cliniques Universitaires St. Luc UCL, Av Hippocrate 10 / 2806, B-1200, Woluwe St. Lambert, Belgium. Bernhard.gerber@uclouvain.be. AD - Pole de Recherche Cardiovasculaire (CARD), Institut de Recherche Experimentale et Clinique (IREC), Universite Catholique de Louvain, Brussels, Belgium. Bernhard.gerber@uclouvain.be. LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Validation Study DEP - 20150611 PL - England TA - J Cardiovasc Magn Reson JT - Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance JID - 9815616 RN - 0 (Contrast Media) RN - 0 (Organometallic Compounds) RN - 1BJ477IO2L (gadobutrol) SB - IM MH - Adult MH - Aged MH - Aortic Valve Insufficiency/*pathology/physiopathology MH - Aortic Valve Stenosis/*pathology/physiopathology MH - Biopsy MH - Contrast Media MH - Fibrosis MH - Humans MH - Image Interpretation, Computer-Assisted/*methods MH - Magnetic Resonance Imaging/*methods MH - Male MH - Middle Aged MH - Mitral Valve Insufficiency/*pathology/physiopathology MH - Myocardium/*pathology MH - Organometallic Compounds MH - Predictive Value of Tests MH - Reproducibility of Results MH - Severity of Illness Index PMC - PMC4464705 EDAT- 2015/06/13 06:00 MHDA- 2016/03/22 06:00 PMCR- 2015/06/11 CRDT- 2015/06/12 06:00 PHST- 2014/11/06 00:00 [received] PHST- 2015/05/18 00:00 [accepted] PHST- 2015/06/12 06:00 [entrez] PHST- 2015/06/13 06:00 [pubmed] PHST- 2016/03/22 06:00 [medline] PHST- 2015/06/11 00:00 [pmc-release] AID - S1097-6647(23)00873-6 [pii] AID - 150 [pii] AID - 10.1186/s12968-015-0150-0 [doi] PST - epublish SO - J Cardiovasc Magn Reson. 2015 Jun 11;17(1):48. doi: 10.1186/s12968-015-0150-0.