PMID- 26063936 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20150611 LR - 20201001 IS - 0046-8991 (Print) IS - 0973-7715 (Electronic) IS - 0046-8991 (Linking) VI - 55 IP - 3 DP - 2015 Sep TI - Diversity of the Intestinal Bacteria of Cattle Fed on Diets with Different Doses of Gelatinized Starch-Urea. PG - 269-77 LID - 10.1007/s12088-015-0526-8 [doi] AB - Gelatinized starch-urea (Starea, SU) is an effective and economical source of urea for ruminants. Here we assessed the influence of dietary supplementation with gelatinized starch-urea on the diversity of intestinal bacteria in finishing cattle. Fifty steers were randomly allotted to five treatments with diets supplemented with different doses of Starea [0 % (SU0), 8 % (SU8), 16 % (SU16), 24 % (SU24), and 32 % (SU32) of urea-N in total nitrogen]. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes was used to examine the effect of dietary supplementation of Starea on intestinal bacterial flora. Shannon-Weaver and Simpson diversity indices consistently showed the lowest bacterial diversity in the SU0 treatment. Increasing doses of Starea increased the diversity up to SU24 after which, diversity decreased. Cluster analysis of 16S rRNA gene DGGE profiles indicates that the intestinal bacterial communities associated with cattle that were not supplemented with Starea in feed differed in composition and structure from those supplemented with Starea. The amount of Starea supplemented in cattle diets influenced the abundance of several key species affiliated with Lachnospiraceae, Ruminococcaceae, Peptostreptococcaceae, Comamonadaceae and Moraxellaceae. These results suggest that Starea influences the composition and structure of intestinal bacteria which may play a role in promoting ruminant health and production performance. FAU - Cui, Zhenliang AU - Cui Z AD - State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China. FAU - Meng, Qingxiang AU - Meng Q AD - State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China. FAU - Ma, Wei AU - Ma W AD - State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China. FAU - Zhang, Xinzhuang AU - Zhang X AD - State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China. FAU - Zhou, Zhenming AU - Zhou Z AD - State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China. FAU - Ren, Liping AU - Ren L AD - State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China. LA - eng PT - Journal Article DEP - 20150410 PL - India TA - Indian J Microbiol JT - Indian journal of microbiology JID - 0374703 PMC - PMC4456495 OTO - NOTNLM OT - Cattle OT - Denaturing gradient gel electrophoresis OT - Intestinal bacteria OT - Starea OT - Urea EDAT- 2015/06/13 06:00 MHDA- 2015/06/13 06:01 PMCR- 2016/09/01 CRDT- 2015/06/12 06:00 PHST- 2014/12/30 00:00 [received] PHST- 2015/04/03 00:00 [accepted] PHST- 2015/06/12 06:00 [entrez] PHST- 2015/06/13 06:00 [pubmed] PHST- 2015/06/13 06:01 [medline] PHST- 2016/09/01 00:00 [pmc-release] AID - 526 [pii] AID - 10.1007/s12088-015-0526-8 [doi] PST - ppublish SO - Indian J Microbiol. 2015 Sep;55(3):269-77. doi: 10.1007/s12088-015-0526-8. Epub 2015 Apr 10.