PMID- 26063970
OWN - NLM
STAT- MEDLINE
DCOM- 20160212
LR  - 20181113
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2015
DP  - 2015
TI  - Effects of Intravenous Injection of Porphyromonas gingivalis on Rabbit 
      Inflammatory Immune Response and Atherosclerosis.
PG  - 364391
LID - 10.1155/2015/364391 [doi]
LID - 364391
AB  - The effects of intravenous injection of Porphyromonas gingivalis (Pg) on rabbit 
      inflammatory immune response and atherosclerosis were evaluated by establishing a 
      microamount Pg bacteremia model combined with high-fat diet. Twenty-four New 
      Zealand rabbits were randomly divided into Groups A-D (n = 6). After 14 weeks, 
      levels of inflammatory factors (C-reactive protein (CRP), tumor necrosis factor-alpha 
      (TNF-alpha), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1)) in 
      peripheral blood were detected by ELISA. The aorta was subjected to HE staining. 
      Local aortic expressions of toll-like receptor-2 (TLR-2), TLR-4, TNF-alpha, CRP, 
      IL-6, matrix metallopeptidase-9, and MCP-1 were detected by real-time PCR, and 
      those of nuclear factor-kappaB (NF-kappaB) p65, phospho-p38 mitogen-activated protein 
      kinase (MAPK), and phospho-c-Jun N-terminal kinase (JNK) proteins were detected 
      by Western blot. Intravenous injection of Pg to the bloodstream alone induced 
      atherosclerotic changes and significantly increased systemic and local aortic 
      expressions of inflammatory factors, NF-kappaB p65, phospho-p38-MAPK, and JNK, 
      especially in Group D. Injection of microamount Pg induced inflammatory immune 
      response and accelerated atherosclerosis, in which the NF-kappaB p65, p38-MAPK, and 
      JNK signaling pathways played important roles. Intravenous injection of Pg is not 
      the same as Pg from human periodontitis entering the blood stream. Therefore, our 
      results cannot be extrapolated to human periodontitis.
FAU - Lin, Gengbing
AU  - Lin G
AD  - School and Hospital of Stomatology, Fujian Medical University, and Stomatological 
      Key Laboratory of Fujian College and University, Fuzhou 350000, China.
FAU - Chen, Shuai
AU  - Chen S
AD  - School and Hospital of Stomatology, Fujian Medical University, and Stomatological 
      Key Laboratory of Fujian College and University, Fuzhou 350000, China.
FAU - Lei, Lang
AU  - Lei L
AD  - Nanjing Stomatological Hospital, Medical School, Nanjing University, Nanjing 
      210000, China.
FAU - You, Xiaoqing
AU  - You X
AD  - School and Hospital of Stomatology, Fujian Medical University, and Stomatological 
      Key Laboratory of Fujian College and University, Fuzhou 350000, China.
FAU - Huang, Min
AU  - Huang M
AD  - School and Hospital of Stomatology, Fujian Medical University, and Stomatological 
      Key Laboratory of Fujian College and University, Fuzhou 350000, China.
FAU - Luo, Lan
AU  - Luo L
AD  - School and Hospital of Stomatology, Fujian Medical University, and Stomatological 
      Key Laboratory of Fujian College and University, Fuzhou 350000, China.
FAU - Li, Yanfen
AU  - Li Y
AD  - School and Hospital of Stomatology, Fujian Medical University, and Stomatological 
      Key Laboratory of Fujian College and University, Fuzhou 350000, China.
FAU - Zhao, Xin
AU  - Zhao X
AD  - School and Hospital of Stomatology, Fujian Medical University, and Stomatological 
      Key Laboratory of Fujian College and University, Fuzhou 350000, China.
FAU - Yan, Fuhua
AU  - Yan F
AD  - School and Hospital of Stomatology, Fujian Medical University, and Stomatological 
      Key Laboratory of Fujian College and University, Fuzhou 350000, China ; Nanjing 
      Stomatological Hospital, Medical School, Nanjing University, Nanjing 210000, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150503
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Atherosclerosis/*immunology/metabolism
MH  - Bacteremia/*immunology/metabolism
MH  - C-Reactive Protein/metabolism
MH  - Diet, High-Fat
MH  - Injections, Intravenous
MH  - Interleukin-6/metabolism
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Male
MH  - NF-kappa B/metabolism
MH  - Porphyromonas gingivalis/*immunology
MH  - Rabbits
MH  - Toll-Like Receptor 2/metabolism
MH  - Toll-Like Receptor 4/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
PMC - PMC4433690
EDAT- 2015/06/13 06:00
MHDA- 2016/02/13 06:00
PMCR- 2015/05/03
CRDT- 2015/06/12 06:00
PHST- 2015/03/09 00:00 [received]
PHST- 2015/04/15 00:00 [revised]
PHST- 2015/04/15 00:00 [accepted]
PHST- 2015/06/12 06:00 [entrez]
PHST- 2015/06/13 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
PHST- 2015/05/03 00:00 [pmc-release]
AID - 10.1155/2015/364391 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2015;2015:364391. doi: 10.1155/2015/364391. Epub 2015 May 3.