PMID- 26064204
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150611
LR  - 20201001
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 8
IP  - 3
DP  - 2015
TI  - Attenuation of EGFL7 inhibits human laryngocarcinoma cells growth and invasion.
PG  - 3141-55
AB  - AIM: To investigate its effect on the proliferation and invasion of laryngeal 
      carcinoma and understand the potential underlying mechanisms to provide new 
      targets for the diagnosis and treatment of recurrent laryngeal cancer metastasis. 
      METHODS: We constructed a lentiviral vector expressing EGFL7 specific shRNA, and 
      introduced it in EGFL7 functions were attenuated by a lentiviral vector harboring 
      shRNA targeting at EGFL7 in laryngeal carcinoma cell line Hep-2. Prolifereation 
      and invasion assays were carried out in vitro. And in vivo tumor burden assay was 
      done in nude mice. RESULTS: The expression of EGFL7 was knocked-down by 80% in 
      hep-2 cells transfected by the lentiviral EGFL7 shRNA vector and EGFL7 gene 
      expression was detected by realtime PCR and Western blotting analysis 
      respectively. The flow cytometric analysis showed that arrested the cell cycle in 
      G1 phase, In tumor burden assay, to parental And vector control cells, the 
      survival rates Of nude mice in EGFL7 shRNA group dropped down from the first day 
      after implantation as indicated by MTT assay (P < 0.05). The formation and growth 
      rate of xenograft tumor in mice transfected with siRNA against Bmi-1 slowed down 
      significantly. CONCLUSION: Attenuation of EGFL7 function significantly suppresses 
      tumor growth and induces apoptosis, both in vitro and in vivo. EGFL7 may be play 
      a key role in invasion and metastasis of Laryngeal squamous cell carcinoma 
      (LSCC), thus would to be a new target for gene therapy in LSCC.
FAU - Wang, Xiao-Xia
AU  - Wang XX
AD  - Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of 
      Xi'an Jiaotong University Xi'an 710061, Shaanxi, P. R. China ; Department of 
      Otolaryngology, 451 Hospital of People's Liberation Army Xi'an 710054, Shaanxi, 
      P. R. China.
FAU - Yao, Xiao-Bao
AU  - Yao XB
AD  - Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of 
      Xi'an Jiaotong University Xi'an 710061, Shaanxi, P. R. China.
FAU - Qiang, Zhang-Shao
AU  - Qiang ZS
AD  - Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of 
      Xi'an Jiaotong University Xi'an 710061, Shaanxi, P. R. China.
FAU - Zhu, Hong-Liang
AU  - Zhu HL
AD  - Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of 
      Xi'an Jiaotong University Xi'an 710061, Shaanxi, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20150315
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4443038
OTO - NOTNLM
OT  - Apoptosis
OT  - EGFL7
OT  - invasion and metastasis
OT  - laryngeal cancer
OT  - short interfering RNA
EDAT- 2015/06/13 06:00
MHDA- 2015/06/13 06:01
PMCR- 2015/03/15
CRDT- 2015/06/12 06:00
PHST- 2014/12/18 00:00 [received]
PHST- 2015/02/25 00:00 [accepted]
PHST- 2015/06/12 06:00 [entrez]
PHST- 2015/06/13 06:00 [pubmed]
PHST- 2015/06/13 06:01 [medline]
PHST- 2015/03/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2015 Mar 15;8(3):3141-55. eCollection 2015.