PMID- 26064209
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150611
LR  - 20201001
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 8
IP  - 3
DP  - 2015
TI  - Research of osteoblastic induced rat bone marrow mesenchymal stem cells cultured 
      on beta-TCP/PLLA porous scaffold.
PG  - 3202-9
AB  - BACKGROUND: Ceramic and polymer composite scaffolds are widely used in tissue 
      engineering for bone tissue regeneration. Composite of beta-tricalcium phosphate 
      (beta-TCP) and poly L-lactic acid (PLLA), due to its biocompatibility and 
      biodegradability, is widely used in bioengineering. However, optimal ratio, 
      porosity and pore size of this kind of scaffolds were not very clear yet. 
      MATERIALS AND METHODS: We cultured osteoblastic induced rMSCs on beta-TCP/PLLA 
      scaffolds to investigate the optimum construction, which owned better properties 
      for supporting cells growth, proliferation and differentiation. A total of 24 
      mice were divided into three groups: rMSCs + beta-TCP/PLLA, osteoblastic rMSCs + 
      beta-TCP/PLLA and beta-TCP/PLLA without cells. 8 rude mice were implanted with rMSCs + 
      beta-TCP/PLLA in the left thighs and beta-TCP/PLLA without cells in the right thighs. 8 
      rude mice were implanted with osteoblastic rMSCs + beta-TCP/PLLA in the left thighs 
      and the same treatments in the right thighs as the above. After 8 and 12 weeks, 
      the mice were sacrificed and implants with the surrounding tissues were harvested 
      together. Paraffin sections were got and HE stain and Masson-Goldner stain were 
      employed to observe the ectopic bone formation. RESULTS: The scaffolds of 
      beta-TCP/PLLA = 2:1 significantly increased osteocalcin production of the cells. In 
      addition, scaffolds with NaCl = 70 wt%, pore size 200~450 mum showed better 
      compatibility to these seeding cells. A significantly larger area of bone 
      formation in the osteoblastic rMSCs and beta-TCP/PLLA composite than that in 
      rMSCs/scaffold and in the scaffold without cells in vivo. CONCLUSION: compounds 
      of osteoblastic induced rMSCs and the scaffold with beta-TCP/PLLA = 2:1, NaCl = 70 
      wt%, pore size = 200-450 mum had good properties as a kind of bone substitute.
FAU - Yang, Yi
AU  - Yang Y
AD  - Orthopedic Center, The First Affiliated Hospital of Xinjiang Medical University 
      Chengdu 610041, China.
FAU - Wu, Jiang
AU  - Wu J
AD  - School of West China Basic Medicine and Forensic Medicine, Sichuan University, 
      Laboratory of Cardiovascular Disease, West China Hospital of Sichuan University 
      Chengdu 610041, China.
FAU - Jin, Gele
AU  - Jin G
AD  - Orthopedic Center, The First Affiliated Hospital of Xinjiang Medical University 
      Chengdu 610041, China.
FAU - Li, Liang
AU  - Li L
AD  - School of West China Basic Medicine and Forensic Medicine, Sichuan University, 
      Laboratory of Cardiovascular Disease, West China Hospital of Sichuan University 
      Chengdu 610041, China.
FAU - Li, Zhongwei
AU  - Li Z
AD  - Orthopedic Center, The First Affiliated Hospital of Xinjiang Medical University 
      Chengdu 610041, China.
FAU - Li, Cao
AU  - Li C
AD  - Orthopedic Center, The First Affiliated Hospital of Xinjiang Medical University 
      Chengdu 610041, China.
LA  - eng
PT  - Journal Article
DEP - 20150315
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4443043
OTO - NOTNLM
OT  - Mesenchymal stem cells
OT  - bone tissue engineering
OT  - osteoblastic induced
OT  - poly-L-lactic acid (PLLA)
OT  - beta-tricalcium phosphate (beta-TCP)
EDAT- 2015/06/13 06:00
MHDA- 2015/06/13 06:01
PMCR- 2015/03/15
CRDT- 2015/06/12 06:00
PHST- 2014/09/25 00:00 [received]
PHST- 2015/03/05 00:00 [accepted]
PHST- 2015/06/12 06:00 [entrez]
PHST- 2015/06/13 06:00 [pubmed]
PHST- 2015/06/13 06:01 [medline]
PHST- 2015/03/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2015 Mar 15;8(3):3202-9. eCollection 2015.