PMID- 26065617
OWN - NLM
STAT- MEDLINE
DCOM- 20160506
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 179
IP  - 3-4
DP  - 2015 Sep 30
TI  - Effects of porcine epidemic diarrhea virus on porcine monocyte-derived dendritic 
      cells and intestinal dendritic cells.
PG  - 131-41
LID - S0378-1135(15)00212-6 [pii]
LID - 10.1016/j.vetmic.2015.05.016 [doi]
AB  - Infection with porcine epidemic diarrhea virus (PEDV) causes damage to intestinal 
      epithelial cells and results in acute diarrhea and dehydration with high 
      mortality rates in swine. Dendritic cells (DCs) are highly effective 
      antigen-presenting cells widely distributed beneath the intestinal epithelium, 
      thus making them an early target for virus contact. DCs uptake and present viral 
      antigens to T cells, which then initiate a distinct immune response. In this 
      study, we investigated how attenuated PEDV (CV777) affects the function of 
      porcine monocyte-derived dendritic cells (Mo-DCs). Our results show that the 
      expression of Mo-DC surface markers such as SWC3a(+)CD1a(+), SWC3a(+)CD80/86(+) 
      and SWC3a(+)SLA-II-DR(+) is increased after infection with CV777 for 24 h. Mo-DCs 
      infected with CV777 produce higher levels of IL-12 and INF-gamma compared to 
      mock-infected Mo-DCs but the expression profile for IL-10 does not change. 
      Interactions between Mo-DCs and CV777 significantly influence the stimulation of 
      the T cell response in vitro. Consistent with these results, after 48 h of CV777 
      infection, there is enhancement in the ability of porcine intestinal DCs to 
      sample the antigen and activate T-cell proliferation in vivo. The enhancement of 
      sampling and presentation is most pronounced for immature Mo-DCs. These results 
      suggest that CV777 stimulates the ability of Mo-DCs to sample and present 
      antigen. We conclude that CV777 may be a useful vaccine to trigger adaptive 
      immunity.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Gao, Qi
AU  - Gao Q
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, Weigang 1, Jiangsu, PR China.
FAU - Zhao, Shanshan
AU  - Zhao S
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, Weigang 1, Jiangsu, PR China.
FAU - Qin, Tao
AU  - Qin T
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, Weigang 1, Jiangsu, PR China.
FAU - Yin, Yinyan
AU  - Yin Y
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, Weigang 1, Jiangsu, PR China.
FAU - Yang, Qian
AU  - Yang Q
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing 
      Agricultural University, Weigang 1, Jiangsu, PR China. Electronic address: 
      zxbyq@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150527
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antigens, Viral)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 9008-11-1 (Interferons)
SB  - IM
EIN - Vet Microbiol. 2020 Jul;246:108701. PMID: 32605761
MH  - Animals
MH  - Antigens, Viral/*immunology
MH  - Coronavirus Infections/immunology/*veterinary/virology
MH  - Dendritic Cells/immunology/virology
MH  - Female
MH  - Interferons/immunology
MH  - Interleukin-10/immunology
MH  - Interleukin-12/immunology
MH  - Intestines/immunology
MH  - Lymphocyte Activation
MH  - Monocytes/immunology/virology
MH  - Porcine epidemic diarrhea virus/*immunology
MH  - Swine
MH  - Swine Diseases/*immunology/virology
MH  - T-Lymphocytes/immunology
OTO - NOTNLM
OT  - Immunity
OT  - Intestinal DCs
OT  - Monocyte-derived dendritic cells (Mo-DCs)
OT  - Porcine epidemic diarrhea virus
OT  - T cell proliferation
EDAT- 2015/06/13 06:00
MHDA- 2016/05/07 06:00
CRDT- 2015/06/13 06:00
PHST- 2014/11/04 00:00 [received]
PHST- 2015/05/08 00:00 [revised]
PHST- 2015/05/21 00:00 [accepted]
PHST- 2015/06/13 06:00 [entrez]
PHST- 2015/06/13 06:00 [pubmed]
PHST- 2016/05/07 06:00 [medline]
AID - S0378-1135(15)00212-6 [pii]
AID - 10.1016/j.vetmic.2015.05.016 [doi]
PST - ppublish
SO  - Vet Microbiol. 2015 Sep 30;179(3-4):131-41. doi: 10.1016/j.vetmic.2015.05.016. 
      Epub 2015 May 27.