PMID- 26065672
OWN - NLM
STAT- MEDLINE
DCOM- 20160329
LR  - 20181202
IS  - 1568-5624 (Electronic)
IS  - 0920-5063 (Linking)
VI  - 26
IP  - 12
DP  - 2015
TI  - Physicomechanical properties of sintered scaffolds formed from porous and 
      protein-loaded poly(DL-lactic-co-glycolic acid) microspheres for potential use in 
      bone tissue engineering.
PG  - 796-811
LID - 10.1080/09205063.2015.1058696 [doi]
AB  - An injectable poly(DL-lactic-co-glycolic acid) (PLGA) system comprising both 
      porous and protein-loaded microspheres capable of forming porous scaffolds at 
      body temperature was developed for tissue regeneration purposes. Porous and 
      non-porous (lysozyme loaded) PLGA microspheres were formulated to represent 'low 
      molecular weight' 22-34 kDa, 'intermediate molecular weight' (IMW) 53 kDa and 
      'high molecular weight' 84-109 kDa PLGA microspheres. The respective average size 
      of the microspheres was directly related to the polymer molecular weight. An 
      initial burst release of lysozyme was observed from both microspheres and 
      scaffolds on day 1. In the case of the lysozyme-loaded microspheres, this burst 
      release was inversely related to the polymer molecular weight. Similarly, 
      scaffolds loaded with 1 mg lysozyme/g of scaffold exhibited an inverse release 
      relationship with polymer molecular weight. The burst release was highest amongst 
      IMW scaffolds loaded with 2 and 3 mg/g. Sustained lysozyme release was observed 
      after day 1 over 50 days (microspheres) and 30 days (scaffolds). The compressive 
      strengths of the scaffolds were found to be inversely proportional to PLGA 
      molecular weight at each lysozyme loading. Surface analysis indicated that some 
      of the loaded lysozyme was distributed on the surfaces of the microspheres and 
      thus responsible for the burst release observed. Overall the data demonstrates 
      the potential of the scaffolds for use in tissue regeneration.
FAU - Boukari, Yamina
AU  - Boukari Y
AD  - a School of Pharmacy , The University of Nottingham Malaysia Campus , 43500 
      Semenyih , Selangor , Malaysia.
FAU - Scurr, David J
AU  - Scurr DJ
FAU - Qutachi, Omar
AU  - Qutachi O
FAU - Morris, Andrew P
AU  - Morris AP
FAU - Doughty, Stephen W
AU  - Doughty SW
FAU - Rahman, Cheryl V
AU  - Rahman CV
FAU - Billa, Nashiru
AU  - Billa N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150702
PL  - England
TA  - J Biomater Sci Polym Ed
JT  - Journal of biomaterials science. Polymer edition
JID - 9007393
RN  - 0 (Drug Carriers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Animals
MH  - Compressive Strength
MH  - Drug Carriers/chemistry
MH  - Drug Liberation
MH  - Femur/*cytology
MH  - Lactic Acid/*chemistry
MH  - Materials Testing
MH  - *Mechanical Phenomena
MH  - *Microspheres
MH  - Molecular Weight
MH  - Muramidase/*chemistry
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Porosity
MH  - Rats
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - BMP-2
OT  - PLGA
OT  - injectable
OT  - lysozyme
OT  - microsphere
OT  - porous
OT  - scaffold
OT  - tissue engineering
EDAT- 2015/06/13 06:00
MHDA- 2016/03/30 06:00
CRDT- 2015/06/13 06:00
PHST- 2015/06/13 06:00 [entrez]
PHST- 2015/06/13 06:00 [pubmed]
PHST- 2016/03/30 06:00 [medline]
AID - 10.1080/09205063.2015.1058696 [doi]
PST - ppublish
SO  - J Biomater Sci Polym Ed. 2015;26(12):796-811. doi: 10.1080/09205063.2015.1058696. 
      Epub 2015 Jul 2.