PMID- 26068081
OWN - NLM
STAT- MEDLINE
DCOM- 20160620
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Jun 12
TI  - Generation of a variety of stable Influenza A reporter viruses by genetic 
      engineering of the NS gene segment.
PG  - 11346
LID - 10.1038/srep11346 [doi]
LID - 11346
AB  - Influenza A viruses (IAV) pose a constant threat to the human population and 
      therefore a better understanding of their fundamental biology and identification 
      of novel therapeutics is of upmost importance. Various reporter-encoding IAV were 
      generated to achieve these goals, however, one recurring difficulty was the 
      genetic instability especially of larger reporter genes. We employed the viral NS 
      segment coding for the non-structural protein 1 (NS1) and nuclear export protein 
      (NEP) for stable expression of diverse reporter proteins. This was achieved by 
      converting the NS segment into a single open reading frame (ORF) coding for NS1, 
      the respective reporter and NEP. To allow expression of individual proteins, the 
      reporter genes were flanked by two porcine Teschovirus-1 2A peptide (PTV-1 
      2A)-coding sequences. The resulting viruses encoding luciferases, fluorescent 
      proteins or a Cre recombinase are characterized by a high genetic stability in 
      vitro and in mice and can be readily employed for antiviral compound screenings, 
      visualization of infected cells or cells that survived acute infection.
FAU - Reuther, Peter
AU  - Reuther P
AD  - 1] Institute for Virology, University of Freiburg, 79104 Freiburg, Germany [2] 
      Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.
FAU - Gopfert, Kristina
AU  - Gopfert K
AD  - Institute for Virology, University of Freiburg, 79104 Freiburg, Germany.
FAU - Dudek, Alexandra H
AU  - Dudek AH
AD  - 1] Institute for Virology, University of Freiburg, 79104 Freiburg, Germany [2] 
      Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany [3].
FAU - Heiner, Monika
AU  - Heiner M
FAU - Herold, Susanne
AU  - Herold S
FAU - Schwemmle, Martin
AU  - Schwemmle M
AD  - Institute for Virology, University of Freiburg, 79104 Freiburg, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150612
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (INS1 protein, influenza virus)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Dogs
MH  - *Genes, Reporter
MH  - HEK293 Cells
MH  - Humans
MH  - *Influenza A virus/genetics/metabolism
MH  - *Luciferases/biosynthesis/genetics
MH  - Madin Darby Canine Kidney Cells
MH  - Mice
MH  - *Viral Nonstructural Proteins/genetics/metabolism
PMC - PMC4464305
EDAT- 2015/06/13 06:00
MHDA- 2016/06/21 06:00
PMCR- 2015/06/12
CRDT- 2015/06/13 06:00
PHST- 2015/01/21 00:00 [received]
PHST- 2015/05/21 00:00 [accepted]
PHST- 2015/06/13 06:00 [entrez]
PHST- 2015/06/13 06:00 [pubmed]
PHST- 2016/06/21 06:00 [medline]
PHST- 2015/06/12 00:00 [pmc-release]
AID - srep11346 [pii]
AID - 10.1038/srep11346 [doi]
PST - epublish
SO  - Sci Rep. 2015 Jun 12;5:11346. doi: 10.1038/srep11346.