PMID- 26070209
OWN - NLM
STAT- MEDLINE
DCOM- 20160406
LR  - 20240326
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 6
DP  - 2015
TI  - RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on 
      Chemoreceptors.
PG  - e0128951
LID - 10.1371/journal.pone.0128951 [doi]
LID - e0128951
AB  - The chemosensory capacity of the somatosensory system relies on the appropriate 
      expression of chemoreceptors, which detect chemical stimuli and transduce sensory 
      information into cellular signals. Knowledge of the complete repertoire of the 
      chemoreceptors expressed in human sensory ganglia is lacking. This study employed 
      the next-generation sequencing technique (RNA-Seq) to conduct the first 
      expression analysis of human trigeminal ganglia (TG) and dorsal root ganglia 
      (DRG). We analyzed the data with a focus on G-protein coupled receptors (GPCRs) 
      and ion channels, which are (potentially) involved in chemosensation by 
      somatosensory neurons in the human TG and DRG. For years, transient receptor 
      potential (TRP) channels have been considered the main group of receptors for 
      chemosensation in the trigeminal system. Interestingly, we could show that 
      sensory ganglia also express a panel of different olfactory receptors (ORs) with 
      putative chemosensory function. To characterize OR expression in more detail, we 
      performed microarray, semi-quantitative RT-PCR experiments, and 
      immunohistochemical staining. Additionally, we analyzed the expression data to 
      identify further known or putative classes of chemoreceptors in the human TG and 
      DRG. Our results give an overview of the major classes of chemoreceptors 
      expressed in the human TG and DRG and provide the basis for a broader 
      understanding of the reception of chemical cues.
FAU - Flegel, Caroline
AU  - Flegel C
AD  - Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
FAU - Schobel, Nicole
AU  - Schobel N
AD  - Department of Animal Physiology, Ruhr-University Bochum, Bochum, Germany.
FAU - Altmuller, Janine
AU  - Altmuller J
AD  - Cologne Center for Genomics, University of Koln, Koln, Germany.
FAU - Becker, Christian
AU  - Becker C
AD  - Cologne Center for Genomics, University of Koln, Koln, Germany.
FAU - Tannapfel, Andrea
AU  - Tannapfel A
AD  - Institute of Pathology, Ruhr-University Bochum, Bochum, Germany.
FAU - Hatt, Hanns
AU  - Hatt H
AD  - Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
FAU - Gisselmann, Gunter
AU  - Gisselmann G
AD  - Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150612
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Receptors, Odorant)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Ganglia, Spinal/cytology/*metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - RNA/*biosynthesis/genetics
MH  - Receptors, Odorant/*biosynthesis/genetics
MH  - Sequence Analysis, RNA
MH  - Trigeminal Ganglion/cytology/*metabolism
PMC - PMC4466559
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/06/13 06:00
MHDA- 2016/04/07 06:00
PMCR- 2015/06/12
CRDT- 2015/06/13 06:00
PHST- 2015/01/26 00:00 [received]
PHST- 2015/05/01 00:00 [accepted]
PHST- 2015/06/13 06:00 [entrez]
PHST- 2015/06/13 06:00 [pubmed]
PHST- 2016/04/07 06:00 [medline]
PHST- 2015/06/12 00:00 [pmc-release]
AID - PONE-D-15-03204 [pii]
AID - 10.1371/journal.pone.0128951 [doi]
PST - epublish
SO  - PLoS One. 2015 Jun 12;10(6):e0128951. doi: 10.1371/journal.pone.0128951. 
      eCollection 2015.