PMID- 26070945
OWN - NLM
STAT- MEDLINE
DCOM- 20160310
LR  - 20181113
IS  - 1756-3305 (Electronic)
IS  - 1756-3305 (Linking)
VI  - 8
DP  - 2015 Jun 14
TI  - L-Arginine supplementation in mice enhances NO production in spleen cells and 
      inhibits Plasmodium yoelii transmission in mosquitoes.
PG  - 326
LID - 10.1186/s13071-015-0940-0 [doi]
LID - 326
AB  - BACKGROUND: The life cycle of Plasmodium is complex, requiring invasion of two 
      different hosts, humans and mosquitoes. In humans, initiation of an effective Th1 
      response during early infection is critical for the control of parasite 
      multiplication. In mosquitoes, inhibition of the development of sexual-stage 
      parasites interrupts the parasite transmission. In this study, we aim to 
      investigate whether dietary supplementation of L-arginine (L-Arg) in mice affects 
      Plasmodium yoelii 17XL (Py17XL) transmission in mosquitoes. METHODS: BALB/c mice 
      were orally administered with 1.5 mg/g L-Arg daily for 7 days and infected with 
      Py17XL. The mRNA levels of inducible nitric oxide synthase (iNOS) and arginase 1 
      in spleen cells were determined by real-time RT-PCR. The amount of nitric oxide 
      (NO) released by spleen cells in vitro was determined by the Griess method. The 
      effect of L-Arg supplementation on subsequent development of P. yoelii 
      gametocytes was evaluated by an in vitro ookinete culture assay and mosquito 
      feeding assay. RESULTS: Pretreatment of mice with L-Arg significantly increased 
      the transcript level of iNOS in spleen cells and the amount of NO synthesized. 
      Dietary L-Arg supplementation also significantly reduced the number of zygotes 
      and ookinetes formed during in vitro culture and the number of oocysts formed on 
      mosquito midguts after blood feeding. CONCLUSIONS: L-Arg enhances host immunity 
      against blood-stage parasites as well as suppressing subsequent parasite 
      development in mosquitoes. L-Arg as an inexpensive and safe supplement may be 
      used as a novel adjunct treatment against malarial infection.
FAU - Zheng, Li
AU  - Zheng L
AD  - Department of Immunology, College of Basic Medical Sciences, China Medical 
      University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning, 110122, 
      P. R. China. zhengli@mail.cmu.edu.cn.
FAU - Pan, Yanyan
AU  - Pan Y
AD  - Department of Immunology, College of Basic Medical Sciences, China Medical 
      University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning, 110122, 
      P. R. China. 66848464@qq.com.
FAU - Feng, Yonghui
AU  - Feng Y
AD  - Department of Immunology, College of Basic Medical Sciences, China Medical 
      University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning, 110122, 
      P. R. China. 156815643@qq.com.
FAU - Cui, Liwang
AU  - Cui L
AD  - Department of Immunology, College of Basic Medical Sciences, China Medical 
      University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning, 110122, 
      P. R. China. luc2@psu.edu.
AD  - Department of Entomology, Pennsylvania State University, University Park, PA, 
      USA. luc2@psu.edu.
FAU - Cao, Yaming
AU  - Cao Y
AD  - Department of Immunology, College of Basic Medical Sciences, China Medical 
      University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning, 110122, 
      P. R. China. ymcao@mail.cmu.edu.cn.
LA  - eng
GR  - R01 AI099611/AI/NIAID NIH HHS/United States
GR  - U19 AI089672/AI/NIAID NIH HHS/United States
GR  - R01AI099611/AI/NIAID NIH HHS/United States
GR  - U19AI089672/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150614
PL  - England
TA  - Parasit Vectors
JT  - Parasites & vectors
JID - 101462774
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Animals
MH  - Anopheles/parasitology/*physiology
MH  - Arginine/*metabolism
MH  - Female
MH  - Humans
MH  - Insect Vectors/parasitology/*physiology
MH  - Malaria/*metabolism/parasitology/transmission
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nitric Oxide/*metabolism
MH  - Plasmodium yoelii/growth & development/*physiology
MH  - Spleen/*metabolism
PMC - PMC4468801
EDAT- 2015/06/14 06:00
MHDA- 2016/03/11 06:00
PMCR- 2015/06/14
CRDT- 2015/06/14 06:00
PHST- 2015/04/08 00:00 [received]
PHST- 2015/06/05 00:00 [accepted]
PHST- 2015/06/14 06:00 [entrez]
PHST- 2015/06/14 06:00 [pubmed]
PHST- 2016/03/11 06:00 [medline]
PHST- 2015/06/14 00:00 [pmc-release]
AID - 10.1186/s13071-015-0940-0 [pii]
AID - 940 [pii]
AID - 10.1186/s13071-015-0940-0 [doi]
PST - epublish
SO  - Parasit Vectors. 2015 Jun 14;8:326. doi: 10.1186/s13071-015-0940-0.