PMID- 26073049
OWN - NLM
STAT- MEDLINE
DCOM- 20151217
LR  - 20171116
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 63
IP  - 26
DP  - 2015 Jul 8
TI  - Aflatoxin B1 Suppressed T-Cell Response to Anti-pig-CD3 Monoclonal Antibody 
      Stimulation in Primary Porcine Splenocytes: A Role for the Extracellular 
      Regulated Protein Kinase (ERK1/2) MAPK Signaling Pathway.
PG  - 6094-101
LID - 10.1021/acs.jafc.5b00433 [doi]
AB  - The aim of the present study is to investigate whether aflatoxin B1 
      (AFB1)-induced immunotoxicity is associated with oxidative stress and the 
      expression of extracellular regulated protein kinases (ERK) 1/2. The primary 
      splenocytes isolated from healthy pigs were activated and proliferated by 
      anti-pig-CD3 monoclonal antibodies (mAb) in the present experiment, which is an 
      antigen-specific stimulant. Results indicated that cell proliferation and 
      interleukin-2 (IL-2) production were significantly suppressed by AFB1 from 4 to 8 
      mug/mL in a dose-dependent manner compared to the control group. Furthermore, AFB1 
      significantly increased malondialdehyde (MDA) levels, decreased reduced 
      glutathione (GSH) and total superoxide dismutase levels, and up-regulated 
      p-ERK1/2 expression in the activated splenocytes. N-Acetyl-l-cysteine blocked 
      anti-CD3-induced T-cell suppression by AFB1 through increasing intracellular 
      concentrations of GSH levels, decreasing MDA levels, and down-regulated p-ERK1/2 
      expression, respectively. Inhibition of the ERK1/2 expression by ERK-specific 
      iRNA attenuated the decrease of T-cell proliferation and IL-2 production induced 
      by AFB1. It was concluded that AFB1 inhibits anti-CD3-induced lymphocyte 
      proliferation and IL-2 production by the oxidative stress mediated ERK1/2 MAPK 
      signaling pathway.
FAU - Hao, Shu
AU  - Hao S
AD  - Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, 
      Nanjing Agricultural University, Nanjing 210095, China.
FAU - Pan, Shengchi
AU  - Pan S
AD  - Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, 
      Nanjing Agricultural University, Nanjing 210095, China.
FAU - Hu, Junfa
AU  - Hu J
AD  - Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, 
      Nanjing Agricultural University, Nanjing 210095, China.
FAU - Qian, Gang
AU  - Qian G
AD  - Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, 
      Nanjing Agricultural University, Nanjing 210095, China.
FAU - Gan, Fang
AU  - Gan F
AD  - Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, 
      Nanjing Agricultural University, Nanjing 210095, China.
FAU - Huang, Kehe
AU  - Huang K
AD  - Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, 
      Nanjing Agricultural University, Nanjing 210095, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150624
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD3 Complex)
RN  - 9N2N2Y55MH (Aflatoxin B1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Aflatoxin B1/*toxicity
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - CD3 Complex/*immunology
MH  - MAP Kinase Signaling System/*drug effects
MH  - Mitogen-Activated Protein Kinase 1/genetics/immunology
MH  - Mitogen-Activated Protein Kinase 3/genetics/immunology
MH  - Spleen/*cytology/drug effects/immunology
MH  - Swine
MH  - T-Lymphocytes/drug effects/*immunology
OTO - NOTNLM
OT  - ERK1/2
OT  - RNA interference
OT  - aflatoxin B1
OT  - anti-pig-CD3 mAb
OT  - primary porcine splenocytes
EDAT- 2015/06/16 06:00
MHDA- 2015/12/19 06:00
CRDT- 2015/06/16 06:00
PHST- 2015/06/16 06:00 [entrez]
PHST- 2015/06/16 06:00 [pubmed]
PHST- 2015/12/19 06:00 [medline]
AID - 10.1021/acs.jafc.5b00433 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2015 Jul 8;63(26):6094-101. doi: 10.1021/acs.jafc.5b00433. 
      Epub 2015 Jun 24.