PMID- 26074310
OWN - NLM
STAT- MEDLINE
DCOM- 20160301
LR  - 20181202
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 120
DP  - 2015 Oct
TI  - Toxicogenetic effects of low concentrations of the pesticides imidacloprid and 
      sulfentrazone individually and in combination in in vitro tests with HepG2 cells 
      and Salmonella typhimurium.
PG  - 174-83
LID - S0147-6513(15)00263-8 [pii]
LID - 10.1016/j.ecoenv.2015.05.040 [doi]
AB  - The insecticide imidacloprid and the herbicide sulfentrazone are two different 
      classes of pesticides that are used for pest control in sugarcane agriculture. To 
      evaluate the genotoxic potential of low concentrations of these two pesticides 
      alone and in mixture, the comet assay and the micronucleus (MN) test employing 
      fluorescence in situ hybridization (FISH) with a centromeric probe were applied 
      in human hepatoma cell lines (HepG2), in a 24-h assay. Mutagenicity was assessed 
      by Salmonella/microsome assay with TA98 and TA100 strains in the absence and 
      presence of an exogenous metabolizing system (S9). The results showed significant 
      inductions of MN in HepG2 cells by both pesticides, for all the tested 
      concentrations. As evidenced in the comet assay, only the imidacloprid presented 
      significant responses. When the two pesticides were associated, a significant 
      induction of damage was observed in the HepG2 cells by the comet assay, but not 
      by the MN test. Moreover, the MN induced by the mixtures of the pesticides 
      appeared at lower levels than those induced by sulfentrazone and imidacloprid 
      when tested alone. According to the FISH results, the damage induced by 
      imidacloprid in the HepG2 cells resulted from a clastogenic action of this 
      insecticide (76.6% of the MN did not present a centromeric signal). For the 
      herbicide sulfentrazone and for the mixture of the pesticides, a similar 
      frequency of MN with and without the presence of the centromeric signal 
      (herbicide: 52.45% of the MN without centromeric signal and 47.54% of the MN with 
      centromeric signal; mixture: 48.71% of the MN without centromeric signal and 
      51.42% of the MN with centromeric signal) was verified. Based on these results, 
      it was concluded that each one of the pesticides evaluated interacts with the DNA 
      of HepG2 cells and causes irreparable alterations in the cells. However, the 
      combination of the pesticides showed an antagonistic effect on the cells and the 
      damage induced was milder and not persistent in HepG2 cells. The results obtained 
      by the Ames test did not point out significant results.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Bianchi, Jaqueline
AU  - Bianchi J
AD  - Department of Biology, Institute of Biosciences, Sao Paulo State University 
      (UNESP), Av. 24A, 1515, Bela Vista, Rio Claro, Sao Paulo CEP 13506-900, Brazil.
FAU - Cabral-de-Mello, Diogo Cavalcanti
AU  - Cabral-de-Mello DC
AD  - Department of Biology, Institute of Biosciences, Sao Paulo State University 
      (UNESP), Av. 24A, 1515, Bela Vista, Rio Claro, Sao Paulo CEP 13506-900, Brazil.
FAU - Marin-Morales, Maria Aparecida
AU  - Marin-Morales MA
AD  - Department of Biology, Institute of Biosciences, Sao Paulo State University 
      (UNESP), Av. 24A, 1515, Bela Vista, Rio Claro, Sao Paulo CEP 13506-900, Brazil. 
      Electronic address: mamm@rc.unesp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150612
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Imidazoles)
RN  - 0 (Neonicotinoids)
RN  - 0 (Nitro Compounds)
RN  - 0 (Pesticides)
RN  - 0 (Sulfonamides)
RN  - 0 (Triazoles)
RN  - 3BN7M937V8 (imidacloprid)
RN  - 7TY7WT1599 
      (N-(2,4-dichloro-5-(4-(difluoromethyl)-4,5-dihydro-3-methyl-5-oxo-1H-1,2,4-triazol-1-yl)phenyl)methanesulfonamide)
SB  - IM
MH  - Comet Assay
MH  - DNA Damage/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Hep G2 Cells
MH  - Humans
MH  - Imidazoles/*toxicity
MH  - In Situ Hybridization, Fluorescence
MH  - Micronucleus Tests
MH  - Mutagenicity Tests
MH  - Neonicotinoids
MH  - Nitro Compounds/*toxicity
MH  - Pesticides/*toxicity
MH  - Salmonella typhimurium/*drug effects
MH  - Sulfonamides/*toxicity
MH  - Triazoles/*toxicity
OTO - NOTNLM
OT  - Ames test
OT  - Comet assay
OT  - FISH technique
OT  - Herbicide
OT  - Insecticide
OT  - Micronucleus
EDAT- 2015/06/16 06:00
MHDA- 2016/03/02 06:00
CRDT- 2015/06/16 06:00
PHST- 2015/02/03 00:00 [received]
PHST- 2015/05/22 00:00 [revised]
PHST- 2015/05/26 00:00 [accepted]
PHST- 2015/06/16 06:00 [entrez]
PHST- 2015/06/16 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
AID - S0147-6513(15)00263-8 [pii]
AID - 10.1016/j.ecoenv.2015.05.040 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2015 Oct;120:174-83. doi: 10.1016/j.ecoenv.2015.05.040. 
      Epub 2015 Jun 12.