PMID- 26076449
OWN - NLM
STAT- MEDLINE
DCOM- 20160304
LR  - 20181202
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 6
DP  - 2015
TI  - Recombinant Encephalomyocarditis Viruses Elicit Neutralizing Antibodies against 
      PRRSV and CSFV in Mice.
PG  - e0129729
LID - 10.1371/journal.pone.0129729 [doi]
LID - e0129729
AB  - Encephalomyocarditis virus (EMCV) is capable of infecting a wide range of species 
      and the infection can cause myocarditis and reproductive failure in pigs as well 
      as febrile illness in human beings. In this study, we introduced the entire ORF5 
      of the porcine reproductive and respiratory syndrome virus (PRRSV) or the 
      neutralization epitope regions in the E2 gene of the classical swine fever virus 
      (CSFV), into the genome of a stably attenuated EMCV strain, T1100I. The resultant 
      viable recombinant viruses, CvBJC3m/I-DeltaGP5 and CvBJC3m/I-E2, respectively 
      expressed partial PRRSV envelope protein GP5 or CSFV neutralization epitope A1A2 
      along with EMCV proteins. These heterologous proteins fused to the N-terminal of 
      the nonstructural leader protein could be recognized by anti-GP5 or anti-E2 
      antibody. We also tested the immunogenicity of these fusion proteins by 
      immunizing BALB/c mice with the recombinant viruses. The immunized animals 
      elicited neutralizing antibodies against PRRSV and CSFV. Our results suggest that 
      EMCV can be engineered as an expression vector and serve as a tool in the 
      development of novel live vaccines in various animal species.
FAU - Zhu, Shu
AU  - Zhu S
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - Guo, Xin
AU  - Guo X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - Keyes, Lisa R
AU  - Keyes LR
AD  - Department of Molecular Genetics and Microbiology, College of Medicine, 
      University of Florida, Gainesville, Florida, United States of America.
FAU - Yang, Hanchun
AU  - Yang H
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - Ge, Xinna
AU  - Ge X
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150615
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/*immunology
MH  - Antibodies, Viral/*immunology
MH  - Cell Line
MH  - Classical Swine Fever Virus/*immunology
MH  - Cross Reactions/*immunology
MH  - Encephalomyocarditis virus/genetics/*immunology
MH  - Gene Expression
MH  - Humans
MH  - Immunization
MH  - Mice
MH  - Mutation
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Recombination, Genetic
MH  - Viral Proteins/genetics/immunology
MH  - Virus Replication
PMC - PMC4468123
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/06/16 06:00
MHDA- 2016/03/05 06:00
PMCR- 2015/06/15
CRDT- 2015/06/16 06:00
PHST- 2015/01/19 00:00 [received]
PHST- 2015/05/12 00:00 [accepted]
PHST- 2015/06/16 06:00 [entrez]
PHST- 2015/06/16 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PHST- 2015/06/15 00:00 [pmc-release]
AID - PONE-D-15-02525 [pii]
AID - 10.1371/journal.pone.0129729 [doi]
PST - epublish
SO  - PLoS One. 2015 Jun 15;10(6):e0129729. doi: 10.1371/journal.pone.0129729. 
      eCollection 2015.