PMID- 26079056
OWN - NLM
STAT- MEDLINE
DCOM- 20151124
LR  - 20220408
IS  - 1872-7786 (Electronic)
IS  - 0009-2797 (Linking)
VI  - 239
DP  - 2015 Sep 5
TI  - Chlorpyrifos is estrogenic and alters embryonic hatching, cell proliferation and 
      apoptosis in zebrafish.
PG  - 26-33
LID - S0009-2797(15)00238-0 [pii]
LID - 10.1016/j.cbi.2015.06.010 [doi]
AB  - The potential interference of endocrine disrupting chemicals (EDCs) on aquatic 
      animals and humans has drawn wide attention in recent years. Reports have shown 
      that some organophosphorus pesticides were a kind of EDCs, but their effects on 
      fish species are still under research. In present study, flow cytometry data of 
      HEC-1B cell line showed that chlorpyrifos (CPF) could increase cell proliferation 
      index like 17beta-estradiol (E2), but the effect of CPF was weaker than of E2 in the 
      same concentration. Moreover, CPF altered the expression pattern of 
      estrogen-responsive gene VTG and ERalpha in zebrafish embryos. When exposed to CPF at 
      various concentrations (0, 0.10, 0.25, 0.50, 0.75 and 1.00mg/L) for 48h during 
      the embryo stage, compared with controls, the hatching rate of treated groups 
      significantly increased at the same time and the hatching rate of embryos was 
      proportional to CPF concentration. The mRNA expression levels of c-myc, cyclin 
      D1, Bax and Bcl-2, which are closely related to cell proliferation and cell 
      apoptosis, were disturbed by CPF in zebrafish embryos after exposure treated for 
      48h. In addition, acridine orange (AO) staining of zebrafish embryos showed that 
      cell apoptosis was appeared in the 0.75, 1.00mg/L CPF treated groups. Taken 
      together, the results obtained in the present study indicated that chlorpyrifos 
      is estrogenic and alters embryonic hatching, cell proliferation and apoptosis in 
      zebrafish.
CI  - Copyright (c) 2015. Published by Elsevier Ireland Ltd.
FAU - Yu, Kaimin
AU  - Yu K
AD  - Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Li, Guochao
AU  - Li G
AD  - Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Feng, Weimin
AU  - Feng W
AD  - Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Liu, Lili
AU  - Liu L
AD  - Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Zhang, Jiayu
AU  - Zhang J
AD  - Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Wu, Wei
AU  - Wu W
AD  - Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Xu, Lei
AU  - Xu L
AD  - Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Yan, Yanchun
AU  - Yan Y
AD  - Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China. 
      Electronic address: yanyanchun@caas.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150612
PL  - Ireland
TA  - Chem Biol Interact
JT  - Chemico-biological interactions
JID - 0227276
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Zebrafish Proteins)
RN  - JCS58I644W (Chlorpyrifos)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects/genetics
MH  - Cell Line, Tumor/drug effects
MH  - Cell Proliferation/drug effects/genetics
MH  - Chlorpyrifos/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Embryo, Nonmammalian/cytology/drug effects
MH  - Endocrine Disruptors/*toxicity
MH  - Female
MH  - Flow Cytometry/methods
MH  - Gene Expression Regulation/drug effects
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Humans
MH  - Male
MH  - Toxicity Tests/*methods
MH  - Zebrafish/*embryology
MH  - Zebrafish Proteins/genetics
OTO - NOTNLM
OT  - Apoptosis
OT  - Cell proliferation
OT  - Chlorpyrifos
OT  - Embryonic hatching
OT  - Zebrafish
EDAT- 2015/06/17 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/06/17 06:00
PHST- 2014/09/01 00:00 [received]
PHST- 2015/06/03 00:00 [revised]
PHST- 2015/06/05 00:00 [accepted]
PHST- 2015/06/17 06:00 [entrez]
PHST- 2015/06/17 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0009-2797(15)00238-0 [pii]
AID - 10.1016/j.cbi.2015.06.010 [doi]
PST - ppublish
SO  - Chem Biol Interact. 2015 Sep 5;239:26-33. doi: 10.1016/j.cbi.2015.06.010. Epub 
      2015 Jun 12.