PMID- 26079778
OWN - NLM
STAT- MEDLINE
DCOM- 20151124
LR  - 20230411
IS  - 1945-7197 (Electronic)
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 100
IP  - 8
DP  - 2015 Aug
TI  - Thyroglobulin (Tg) Testing Revisited: Tg Assays, TgAb Assays, and Correlation of 
      Results With Clinical Outcomes.
PG  - E1074-83
LID - 10.1210/jc.2015-1967 [doi]
AB  - CONTEXT: Measurement of thyroglobulin (Tg) by mass spectrometry (Tg-MS) is 
      emerging as a tool for accurate Tg quantification in patients with anti-Tg 
      autoantibodies (TgAbs). OBJECTIVE: The objective of the study was to perform 
      analytical and clinical evaluations of two Tg-MS assays in comparison with 
      immunometric Tg assays (Tg-IAs) and Tg RIAs (Tg-RIAs) in a cohort of thyroid 
      cancer patients. METHODS: A total of 589 samples from 495 patients, 243 TgAb-/252 
      TgAb+, were tested by Beckman, Roche, Siemens-Immulite, and Thermo-Brahms Tg and 
      TgAb assays, two Tg-RIAs, and two Tg-MS assays. RESULTS: The frequency of TgAb+ 
      was 58%, 41%, 27%, and 39% for Roche, Beckman, Siemens-Immulite, and 
      Thermo-Brahms, respectively. In TgAb- samples, clinical sensitivities and 
      specificities of 100% and 74%-100%, respectively, were observed across all 
      assays. In TgAb+ samples, all Tg-IAs demonstrated assay-dependent Tg 
      underestimation, ranging from 41% to 86%. In TgAb+ samples, the use of a common 
      cutoff (0.5 ng/mL) for the Tg-MS, three Tg-IAs, and the USC-RIA improved the 
      sensitivity for the Tg-MSs and Tg-RIAs when compared with the Tg-IAs. In up to 
      20% of TgAb+ cases, Tg-IAs failed to detect Tg that was detectable by Tg-MS. In 
      Tg-RIAs false-high biases were observed in TgAb+ samples containing low Tg 
      concentrations. CONCLUSIONS: Tg-IAs remain the method of choice for Tg 
      quantitation in TgAb- patients. In TgAb+ patients with undetectable Tg by 
      immunometric assay, the Tg-MS will detect Tg in up to 20% additional cases. The 
      Tg-RIA will detect Tg in approximately 35% cases, but a significant proportion of 
      these will be clinical false-positive results. The undetectable Tg-MS seen in 
      approximately 40% of TgAb+ cases in patients with disease need further 
      evaluation.
FAU - Netzel, Brian C
AU  - Netzel BC
AD  - Department of Laboratory Medicine and Pathology (B.C.N., S.K.G.G., A.A.-S.), 
      Division of Endocrinology, Metabolism, and Nutrition (S.K.G.G., B.G.C.L., M.R.C., 
      A.F.T.), Mayo Clinic, Rochester, Minnesota 55905; Queen Elizabeth Hospital 
      Birmingham (P.M.C.), Birmingham B15 2TH, United Kingdom; Department of Laboratory 
      Medicine (A.N.H.), University of Washington, Seattle, Washington 98108; and 
      University of Southern California (C.A.S.), Los Angeles, California 90089.
FAU - Grebe, Stefan K G
AU  - Grebe SK
AD  - Department of Laboratory Medicine and Pathology (B.C.N., S.K.G.G., A.A.-S.), 
      Division of Endocrinology, Metabolism, and Nutrition (S.K.G.G., B.G.C.L., M.R.C., 
      A.F.T.), Mayo Clinic, Rochester, Minnesota 55905; Queen Elizabeth Hospital 
      Birmingham (P.M.C.), Birmingham B15 2TH, United Kingdom; Department of Laboratory 
      Medicine (A.N.H.), University of Washington, Seattle, Washington 98108; and 
      University of Southern California (C.A.S.), Los Angeles, California 90089.
FAU - Carranza Leon, B Gisella
AU  - Carranza Leon BG
AD  - Department of Laboratory Medicine and Pathology (B.C.N., S.K.G.G., A.A.-S.), 
      Division of Endocrinology, Metabolism, and Nutrition (S.K.G.G., B.G.C.L., M.R.C., 
      A.F.T.), Mayo Clinic, Rochester, Minnesota 55905; Queen Elizabeth Hospital 
      Birmingham (P.M.C.), Birmingham B15 2TH, United Kingdom; Department of Laboratory 
      Medicine (A.N.H.), University of Washington, Seattle, Washington 98108; and 
      University of Southern California (C.A.S.), Los Angeles, California 90089.
FAU - Castro, M Regina
AU  - Castro MR
AD  - Department of Laboratory Medicine and Pathology (B.C.N., S.K.G.G., A.A.-S.), 
      Division of Endocrinology, Metabolism, and Nutrition (S.K.G.G., B.G.C.L., M.R.C., 
      A.F.T.), Mayo Clinic, Rochester, Minnesota 55905; Queen Elizabeth Hospital 
      Birmingham (P.M.C.), Birmingham B15 2TH, United Kingdom; Department of Laboratory 
      Medicine (A.N.H.), University of Washington, Seattle, Washington 98108; and 
      University of Southern California (C.A.S.), Los Angeles, California 90089.
FAU - Clark, Penelope M
AU  - Clark PM
AD  - Department of Laboratory Medicine and Pathology (B.C.N., S.K.G.G., A.A.-S.), 
      Division of Endocrinology, Metabolism, and Nutrition (S.K.G.G., B.G.C.L., M.R.C., 
      A.F.T.), Mayo Clinic, Rochester, Minnesota 55905; Queen Elizabeth Hospital 
      Birmingham (P.M.C.), Birmingham B15 2TH, United Kingdom; Department of Laboratory 
      Medicine (A.N.H.), University of Washington, Seattle, Washington 98108; and 
      University of Southern California (C.A.S.), Los Angeles, California 90089.
FAU - Hoofnagle, Andrew N
AU  - Hoofnagle AN
AD  - Department of Laboratory Medicine and Pathology (B.C.N., S.K.G.G., A.A.-S.), 
      Division of Endocrinology, Metabolism, and Nutrition (S.K.G.G., B.G.C.L., M.R.C., 
      A.F.T.), Mayo Clinic, Rochester, Minnesota 55905; Queen Elizabeth Hospital 
      Birmingham (P.M.C.), Birmingham B15 2TH, United Kingdom; Department of Laboratory 
      Medicine (A.N.H.), University of Washington, Seattle, Washington 98108; and 
      University of Southern California (C.A.S.), Los Angeles, California 90089.
FAU - Spencer, Carole A
AU  - Spencer CA
AD  - Department of Laboratory Medicine and Pathology (B.C.N., S.K.G.G., A.A.-S.), 
      Division of Endocrinology, Metabolism, and Nutrition (S.K.G.G., B.G.C.L., M.R.C., 
      A.F.T.), Mayo Clinic, Rochester, Minnesota 55905; Queen Elizabeth Hospital 
      Birmingham (P.M.C.), Birmingham B15 2TH, United Kingdom; Department of Laboratory 
      Medicine (A.N.H.), University of Washington, Seattle, Washington 98108; and 
      University of Southern California (C.A.S.), Los Angeles, California 90089.
FAU - Turcu, Adina F
AU  - Turcu AF
AD  - Department of Laboratory Medicine and Pathology (B.C.N., S.K.G.G., A.A.-S.), 
      Division of Endocrinology, Metabolism, and Nutrition (S.K.G.G., B.G.C.L., M.R.C., 
      A.F.T.), Mayo Clinic, Rochester, Minnesota 55905; Queen Elizabeth Hospital 
      Birmingham (P.M.C.), Birmingham B15 2TH, United Kingdom; Department of Laboratory 
      Medicine (A.N.H.), University of Washington, Seattle, Washington 98108; and 
      University of Southern California (C.A.S.), Los Angeles, California 90089.
FAU - Algeciras-Schimnich, Alicia
AU  - Algeciras-Schimnich A
AD  - Department of Laboratory Medicine and Pathology (B.C.N., S.K.G.G., A.A.-S.), 
      Division of Endocrinology, Metabolism, and Nutrition (S.K.G.G., B.G.C.L., M.R.C., 
      A.F.T.), Mayo Clinic, Rochester, Minnesota 55905; Queen Elizabeth Hospital 
      Birmingham (P.M.C.), Birmingham B15 2TH, United Kingdom; Department of Laboratory 
      Medicine (A.N.H.), University of Washington, Seattle, Washington 98108; and 
      University of Southern California (C.A.S.), Los Angeles, California 90089.
LA  - eng
GR  - P30 DK035816/DK/NIDDK NIH HHS/United States
GR  - U24 CA160034/CA/NCI NIH HHS/United States
GR  - CA160034/CA/NCI NIH HHS/United States
GR  - DK035816/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150616
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Autoantibodies)
RN  - 0 (anti-thyroglobulin)
RN  - 9010-34-8 (Thyroglobulin)
SB  - IM
CIN - J Clin Endocrinol Metab. 2016 Mar;101(3):L38-9. PMID: 26938377
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Autoantibodies/*analysis/blood
MH  - Chromatography, Liquid
MH  - Cohort Studies
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Radioimmunoassay/methods
MH  - Tandem Mass Spectrometry
MH  - Thyroglobulin/*analysis/blood
MH  - *Thyroid Function Tests/methods/standards
MH  - Thyroid Neoplasms/blood/*diagnosis
MH  - Young Adult
PMC - PMC4524993
EDAT- 2015/06/17 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/06/16
CRDT- 2015/06/17 06:00
PHST- 2015/06/17 06:00 [entrez]
PHST- 2015/06/17 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/06/16 00:00 [pmc-release]
AID - 15-1967 [pii]
AID - 10.1210/jc.2015-1967 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2015 Aug;100(8):E1074-83. doi: 10.1210/jc.2015-1967. 
      Epub 2015 Jun 16.