PMID- 26081126
OWN - NLM
STAT- MEDLINE
DCOM- 20160627
LR  - 20181113
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 32
IP  - 7
DP  - 2015 Jul
TI  - Ploidy of spermatogenic cells of men with non-mosaic Klinefelter's syndrome as 
      measured by a computerized cell scanning system.
PG  - 1113-21
LID - 10.1007/s10815-015-0508-0 [doi]
AB  - PURPOSE: This study aims to characterize the origin of testicular post-meiotic 
      cells in non-mosaic Klinefelter's syndrome (KS). METHODS: The study included 
      testicular tissue specimens from 11 non-mosaic KS patients, with (6 positive) and 
      without (5 negative) spermatozoa presence. The obtained testicular cells were 
      affixed and stained for morphology followed by fluorescence in situ hybridization 
      (FISH) for centromeric probes X, Y, and 18. We used a computerized automated cell 
      scanning system that enables simultaneous viewing of morphology and FISH in the 
      same cell. RESULTS: A total of 12,387 cells from the positive cases, 11,991 cells 
      from the negative cases, and 1,711 cells from the controls were analyzed. The 
      majority of spermatogonia were 47, XXY in both the positive and negative KS cases 
      (88.9 +/- 4.76 % and 90.6 +/- 4.58 %) as were primary spermatocytes (76.8 +/- 8.14 % 
      and 79.6 +/- 7.30 %). The respective rates of secondary spermatocytes and 
      post-meiotic cells (round, elongating spermatids and sperm cells) were 1.1 +/- 1.39 
      % in the positive cases, 2.9 +/- 3.33 % in the negative cases, compared to 
      67.6 +/- 6.22 % in the controls (P < 0.02). Pairing of both 18 and XY homologous 
      chromosomes in 46,XY primary spermatocytes was 2.5 +/- 2.31 % and 3.4 +/- 2.39 %, 
      respectively, compared to 19.8 +/- 8.95 % in the control group (P < 0.02) and in 
      47,XXY primary spermatocytes in 2.4 +/- 3.8 % in the positive group and 3.2 +/- 2.26 
      % in the negative group. CONCLUSIONS: This study presents data to indicate that 
      the majority of primary spermatocytes in the testes of non-mosaic KS patients are 
      47,XXY and could possibly develop into post-meiotic cells.
FAU - Komsky-Elbaz, Alisa
AU  - Komsky-Elbaz A
AD  - Infertility and IVF Unit, Assaf Harofeh Medical Center, Zerifin, 703000, Israel.
FAU - Raziel, Arieh
AU  - Raziel A
FAU - Ben-Ami, Ido
AU  - Ben-Ami I
FAU - Bern, Orna
AU  - Bern O
FAU - Maslansky, Billa
AU  - Maslansky B
FAU - Gidoni, Yariv S
AU  - Gidoni YS
FAU - Ron-El, Raphael
AU  - Ron-El R
FAU - Strassburger, Deborah
AU  - Strassburger D
LA  - eng
PT  - Journal Article
DEP - 20150617
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Case-Control Studies
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotype
MH  - Klinefelter Syndrome/*genetics/*pathology
MH  - Male
MH  - *Ploidies
MH  - Spermatocytes/physiology
MH  - Spermatozoa/*pathology/physiology
MH  - Young Adult
PMC - PMC4531865
EDAT- 2015/06/18 06:00
MHDA- 2016/06/28 06:00
PMCR- 2016/07/01
CRDT- 2015/06/18 06:00
PHST- 2015/02/24 00:00 [received]
PHST- 2015/06/02 00:00 [accepted]
PHST- 2015/06/18 06:00 [entrez]
PHST- 2015/06/18 06:00 [pubmed]
PHST- 2016/06/28 06:00 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - 508 [pii]
AID - 10.1007/s10815-015-0508-0 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2015 Jul;32(7):1113-21. doi: 10.1007/s10815-015-0508-0. 
      Epub 2015 Jun 17.