PMID- 26081503
OWN - NLM
STAT- MEDLINE
DCOM- 20160219
LR  - 20231213
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 95
IP  - 16
DP  - 2015 Apr 28
TI  - [Effects of chronic intermittent hypoxia on regulation of miRNA-214 in myocardial 
      apoptosis in rats].
PG  - 1214-7
AB  - OBJECTIVE: To observe the effects of chronic intermittent hypoxia (CIH) on 
      myocardial cell apoptosis and explore the mechanism of microRNA-214 (miRNA-214) 
      expression for myocardial cell apoptosis and cardiac dysfunction. METHODS: A 
      total of 20 adult male Sprague-Dawley rats were randomized into two groups (n = 
      10 each): for CIH group, the rats were raised in a CIH chamber 8 h daily for 6 
      weeks; for normal control (NC) group, the animal were exposed to normoxic air 7 h 
      daily for 6 weeks. Hemodynamic values and myocardial function were measured via a 
      cannula inserted into right common carotid artery. Myocardial cell apoptosis was 
      determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) method. The expressions of miRNA-214 and hypoxia-inducible 
      factor-1alpha (HIF-1alpha) mRNA were observed by real-time polymerase chain reaction 
      (PCR). The expressions of such apoptosis-related proteins as fatty acid synthase 
      (Fas), caspase 3, caspase 8, B-cell lymphoma-2 (Bcl-2) and Bcl-2-Associated X 
      Protein (Bax) were detected by Western blot. RESULTS: Compared with NC group, 
      left ventricle end diastolic pressure (LVEDP) ((4.1 +/- 0.5) vs (2.7 +/- 0.4) mmHg, 1 
      mmHg = 0.133 kPa) increased, maximal rate of pressure decline (-dp/dtmax) ((3 005 
      +/- 86) vs (5 918 +/- 112) mmHg/s), maximal rate of pressure development (+dp/dtmax) 
      ((4 118 +/- 76) vs (7 547 +/- 271) mmHg/s) and left ventricular systolic pressure 
      (LVSP) ((90.7 +/- 2.5) vs (132.7 +/- 5.5) mmHg] decreased in CIH group (all P < 
      0.001). There were significantly more TUNEL positive cells in CIH group versus NC 
      group (P < 0.001). The expression of miRNA-214 was significantly lower in CIH 
      group than that in NC group (P < 0.001). In addition, HIF-1alpha mRNA level was 
      significantly higher in CIH group than that in NC group (P < 0.001). The 
      expressions of caspase 3, caspase 8, Fas and Bax increased significantly in CIH 
      group versus NC group (all P < 0.001), suggesting increased apoptosis of 
      myocardial cells in CIH group. Compared with NC group, the expression of Bcl-2 
      decreased significantly in CIH group (P < 0.001). CONCLUSIONS: CIH may decrease 
      the hemodynamic values and myocardial function in rats. The expression of 
      miRNA-214 in CIH group is significantly depressed through accelerated apoptosis 
      of myocardial cells.
FAU - Chen, Qin
AU  - Chen Q
AD  - Department of Pneumology, Affiliated People's Hospital, Fujian University of 
      Traditional Chinese Medicine, Fuzhou 350000, China.
FAU - Huang, Jiefeng
AU  - Huang J
FAU - Wang, Hao
AU  - Wang H
FAU - Zhao, Jianming
AU  - Zhao J
FAU - Chen, Gongping
AU  - Chen G
FAU - Qi, Jiachao
AU  - Qi J
FAU - Lin, Xin
AU  - Lin X
FAU - Chen, Lida
AU  - Chen L
FAU - Lin, Qichang
AU  - Lin Q
AD  - Department of Pneumology, First Affiliated Hospital, Fujian Medical University, 
      Laboratory of Pneumology, Fujian Medical University, Fujian Provincial 
      Sleep-Disordered Breathing Clinic Center, Fuzhou 350000, China; Email: 
      chang4e@126.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn214 microRNA, rat)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Apoptosis Regulatory Proteins
MH  - Caspase 3
MH  - Chronic Disease
MH  - *Hypoxia
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Male
MH  - MicroRNAs
MH  - *Myocardium
MH  - Myocytes, Cardiac
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - bcl-2-Associated X Protein
EDAT- 2015/06/18 06:00
MHDA- 2016/02/20 06:00
CRDT- 2015/06/18 06:00
PHST- 2015/06/18 06:00 [entrez]
PHST- 2015/06/18 06:00 [pubmed]
PHST- 2016/02/20 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2015 Apr 28;95(16):1214-7.